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An Efficient and Universal Protoplast Isolation Protocol Suitable for Transient Gene Expression Analysis and Single-Cell RNA Sequencing

The recent advent of single-cell RNA sequencing (scRNA-seq) has enabled access to the developmental landscape of a complex organ by monitoring the differentiation trajectory of every specialized cell type at the single-cell level. A main challenge in this endeavor is dissociating plant cells from th...

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Detalles Bibliográficos
Autores principales: Wang, Juanjuan, Wang, Yang, Lü, Tianfeng, Yang, Xia, Liu, Jing, Dong, Yang, Wang, Yinzheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8998730/
https://www.ncbi.nlm.nih.gov/pubmed/35408780
http://dx.doi.org/10.3390/ijms23073419