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Biosynthesis of Rubellins in Ramularia collo-cygni—Genetic Basis and Pathway Proposition
The important disease Ramularia leaf spot of barley is caused by the fungus Ramularia collo-cygni. The disease causes yield and quality losses as a result of a decrease in photosynthesis efficiency due to the appearance of necrotic spots on the leaf surface. The development of these typical Ramulari...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8998751/ https://www.ncbi.nlm.nih.gov/pubmed/35408835 http://dx.doi.org/10.3390/ijms23073475 |
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author | Dussart, Francois Jakubczyk, Dorota |
author_facet | Dussart, Francois Jakubczyk, Dorota |
author_sort | Dussart, Francois |
collection | PubMed |
description | The important disease Ramularia leaf spot of barley is caused by the fungus Ramularia collo-cygni. The disease causes yield and quality losses as a result of a decrease in photosynthesis efficiency due to the appearance of necrotic spots on the leaf surface. The development of these typical Ramularia leaf spot symptoms is thought to be linked with the release of phytotoxic secondary metabolites called rubellins in the host. However, to date, neither the biosynthetic pathways leading to the production of these metabolites nor their exact role in disease development are known. Using a combined in silico genetic and biochemistry approach, we interrogated the genome of R. collo-cygni to identify a putative rubellin biosynthetic gene cluster. Here we report the identification of a gene cluster containing homologues of genes involved in the biosynthesis of related anthraquinone metabolites in closely related fungi. A putative pathway to rubellin biosynthesis involving the genes located on the candidate cluster is also proposed. |
format | Online Article Text |
id | pubmed-8998751 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-89987512022-04-12 Biosynthesis of Rubellins in Ramularia collo-cygni—Genetic Basis and Pathway Proposition Dussart, Francois Jakubczyk, Dorota Int J Mol Sci Communication The important disease Ramularia leaf spot of barley is caused by the fungus Ramularia collo-cygni. The disease causes yield and quality losses as a result of a decrease in photosynthesis efficiency due to the appearance of necrotic spots on the leaf surface. The development of these typical Ramularia leaf spot symptoms is thought to be linked with the release of phytotoxic secondary metabolites called rubellins in the host. However, to date, neither the biosynthetic pathways leading to the production of these metabolites nor their exact role in disease development are known. Using a combined in silico genetic and biochemistry approach, we interrogated the genome of R. collo-cygni to identify a putative rubellin biosynthetic gene cluster. Here we report the identification of a gene cluster containing homologues of genes involved in the biosynthesis of related anthraquinone metabolites in closely related fungi. A putative pathway to rubellin biosynthesis involving the genes located on the candidate cluster is also proposed. MDPI 2022-03-23 /pmc/articles/PMC8998751/ /pubmed/35408835 http://dx.doi.org/10.3390/ijms23073475 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Dussart, Francois Jakubczyk, Dorota Biosynthesis of Rubellins in Ramularia collo-cygni—Genetic Basis and Pathway Proposition |
title | Biosynthesis of Rubellins in Ramularia collo-cygni—Genetic Basis and Pathway Proposition |
title_full | Biosynthesis of Rubellins in Ramularia collo-cygni—Genetic Basis and Pathway Proposition |
title_fullStr | Biosynthesis of Rubellins in Ramularia collo-cygni—Genetic Basis and Pathway Proposition |
title_full_unstemmed | Biosynthesis of Rubellins in Ramularia collo-cygni—Genetic Basis and Pathway Proposition |
title_short | Biosynthesis of Rubellins in Ramularia collo-cygni—Genetic Basis and Pathway Proposition |
title_sort | biosynthesis of rubellins in ramularia collo-cygni—genetic basis and pathway proposition |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8998751/ https://www.ncbi.nlm.nih.gov/pubmed/35408835 http://dx.doi.org/10.3390/ijms23073475 |
work_keys_str_mv | AT dussartfrancois biosynthesisofrubellinsinramulariacollocygnigeneticbasisandpathwayproposition AT jakubczykdorota biosynthesisofrubellinsinramulariacollocygnigeneticbasisandpathwayproposition |