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Biosynthesis of Rubellins in Ramularia collo-cygni—Genetic Basis and Pathway Proposition

The important disease Ramularia leaf spot of barley is caused by the fungus Ramularia collo-cygni. The disease causes yield and quality losses as a result of a decrease in photosynthesis efficiency due to the appearance of necrotic spots on the leaf surface. The development of these typical Ramulari...

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Autores principales: Dussart, Francois, Jakubczyk, Dorota
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8998751/
https://www.ncbi.nlm.nih.gov/pubmed/35408835
http://dx.doi.org/10.3390/ijms23073475
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author Dussart, Francois
Jakubczyk, Dorota
author_facet Dussart, Francois
Jakubczyk, Dorota
author_sort Dussart, Francois
collection PubMed
description The important disease Ramularia leaf spot of barley is caused by the fungus Ramularia collo-cygni. The disease causes yield and quality losses as a result of a decrease in photosynthesis efficiency due to the appearance of necrotic spots on the leaf surface. The development of these typical Ramularia leaf spot symptoms is thought to be linked with the release of phytotoxic secondary metabolites called rubellins in the host. However, to date, neither the biosynthetic pathways leading to the production of these metabolites nor their exact role in disease development are known. Using a combined in silico genetic and biochemistry approach, we interrogated the genome of R. collo-cygni to identify a putative rubellin biosynthetic gene cluster. Here we report the identification of a gene cluster containing homologues of genes involved in the biosynthesis of related anthraquinone metabolites in closely related fungi. A putative pathway to rubellin biosynthesis involving the genes located on the candidate cluster is also proposed.
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spelling pubmed-89987512022-04-12 Biosynthesis of Rubellins in Ramularia collo-cygni—Genetic Basis and Pathway Proposition Dussart, Francois Jakubczyk, Dorota Int J Mol Sci Communication The important disease Ramularia leaf spot of barley is caused by the fungus Ramularia collo-cygni. The disease causes yield and quality losses as a result of a decrease in photosynthesis efficiency due to the appearance of necrotic spots on the leaf surface. The development of these typical Ramularia leaf spot symptoms is thought to be linked with the release of phytotoxic secondary metabolites called rubellins in the host. However, to date, neither the biosynthetic pathways leading to the production of these metabolites nor their exact role in disease development are known. Using a combined in silico genetic and biochemistry approach, we interrogated the genome of R. collo-cygni to identify a putative rubellin biosynthetic gene cluster. Here we report the identification of a gene cluster containing homologues of genes involved in the biosynthesis of related anthraquinone metabolites in closely related fungi. A putative pathway to rubellin biosynthesis involving the genes located on the candidate cluster is also proposed. MDPI 2022-03-23 /pmc/articles/PMC8998751/ /pubmed/35408835 http://dx.doi.org/10.3390/ijms23073475 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Dussart, Francois
Jakubczyk, Dorota
Biosynthesis of Rubellins in Ramularia collo-cygni—Genetic Basis and Pathway Proposition
title Biosynthesis of Rubellins in Ramularia collo-cygni—Genetic Basis and Pathway Proposition
title_full Biosynthesis of Rubellins in Ramularia collo-cygni—Genetic Basis and Pathway Proposition
title_fullStr Biosynthesis of Rubellins in Ramularia collo-cygni—Genetic Basis and Pathway Proposition
title_full_unstemmed Biosynthesis of Rubellins in Ramularia collo-cygni—Genetic Basis and Pathway Proposition
title_short Biosynthesis of Rubellins in Ramularia collo-cygni—Genetic Basis and Pathway Proposition
title_sort biosynthesis of rubellins in ramularia collo-cygni—genetic basis and pathway proposition
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8998751/
https://www.ncbi.nlm.nih.gov/pubmed/35408835
http://dx.doi.org/10.3390/ijms23073475
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