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The Role of Chitooligosaccharidolytic β-N-Acetylglucosamindase in the Molting and Wing Development of the Silkworm Bombyx mori

The insect glycoside hydrolase family 20 β-N-acetylhexosaminidases (HEXs) are key enzymes involved in chitin degradation. In this study, nine HEX genes in Bombyx mori were identified by genome-wide analysis. Bioinformatic analysis based on the transcriptome database indicated that each gene had a di...

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Autores principales: Zhang, Bili, Li, Chunlin, Luan, Yue, Lu, Yaru, Hu, Hai, Liu, Yanyu, Lu, Kunpeng, Zhang, Guizheng, Dai, Fangyin, Tong, Xiaoling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8998872/
https://www.ncbi.nlm.nih.gov/pubmed/35409210
http://dx.doi.org/10.3390/ijms23073850
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author Zhang, Bili
Li, Chunlin
Luan, Yue
Lu, Yaru
Hu, Hai
Liu, Yanyu
Lu, Kunpeng
Zhang, Guizheng
Dai, Fangyin
Tong, Xiaoling
author_facet Zhang, Bili
Li, Chunlin
Luan, Yue
Lu, Yaru
Hu, Hai
Liu, Yanyu
Lu, Kunpeng
Zhang, Guizheng
Dai, Fangyin
Tong, Xiaoling
author_sort Zhang, Bili
collection PubMed
description The insect glycoside hydrolase family 20 β-N-acetylhexosaminidases (HEXs) are key enzymes involved in chitin degradation. In this study, nine HEX genes in Bombyx mori were identified by genome-wide analysis. Bioinformatic analysis based on the transcriptome database indicated that each gene had a distinct expression pattern. qRT-PCR was performed to detect the expression pattern of the chitooligosaccharidolytic β-N-acetylglucosaminidase (BmChiNAG). BmChiNAG was highly expressed in chitin-rich tissues, such as the epidermis. In the wing disc and epidermis, BmChiNAG has the highest expression level during the wandering stage. CRISPR/Cas9-mediated BmChiNAG deletion was used to study the function. In the BmChiNAG-knockout line, 39.2% of female heterozygotes had small and curly wings. The ultrastructure of a cross-section showed that the lack of BmChiNAG affected the stratification of the wing membrane and the formation of the correct wing vein structure. The molting process of the homozygotes was severely hindered during the larva to pupa transition. Epidermal sections showed that the endocuticle of the pupa was not degraded in the mutant. These results indicate that BmChiNAG is involved in chitin catabolism and plays an important role in the molting and wing development of the silkworm, which highlights the potential of BmChiNAG as a pest control target.
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spelling pubmed-89988722022-04-12 The Role of Chitooligosaccharidolytic β-N-Acetylglucosamindase in the Molting and Wing Development of the Silkworm Bombyx mori Zhang, Bili Li, Chunlin Luan, Yue Lu, Yaru Hu, Hai Liu, Yanyu Lu, Kunpeng Zhang, Guizheng Dai, Fangyin Tong, Xiaoling Int J Mol Sci Article The insect glycoside hydrolase family 20 β-N-acetylhexosaminidases (HEXs) are key enzymes involved in chitin degradation. In this study, nine HEX genes in Bombyx mori were identified by genome-wide analysis. Bioinformatic analysis based on the transcriptome database indicated that each gene had a distinct expression pattern. qRT-PCR was performed to detect the expression pattern of the chitooligosaccharidolytic β-N-acetylglucosaminidase (BmChiNAG). BmChiNAG was highly expressed in chitin-rich tissues, such as the epidermis. In the wing disc and epidermis, BmChiNAG has the highest expression level during the wandering stage. CRISPR/Cas9-mediated BmChiNAG deletion was used to study the function. In the BmChiNAG-knockout line, 39.2% of female heterozygotes had small and curly wings. The ultrastructure of a cross-section showed that the lack of BmChiNAG affected the stratification of the wing membrane and the formation of the correct wing vein structure. The molting process of the homozygotes was severely hindered during the larva to pupa transition. Epidermal sections showed that the endocuticle of the pupa was not degraded in the mutant. These results indicate that BmChiNAG is involved in chitin catabolism and plays an important role in the molting and wing development of the silkworm, which highlights the potential of BmChiNAG as a pest control target. MDPI 2022-03-31 /pmc/articles/PMC8998872/ /pubmed/35409210 http://dx.doi.org/10.3390/ijms23073850 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Bili
Li, Chunlin
Luan, Yue
Lu, Yaru
Hu, Hai
Liu, Yanyu
Lu, Kunpeng
Zhang, Guizheng
Dai, Fangyin
Tong, Xiaoling
The Role of Chitooligosaccharidolytic β-N-Acetylglucosamindase in the Molting and Wing Development of the Silkworm Bombyx mori
title The Role of Chitooligosaccharidolytic β-N-Acetylglucosamindase in the Molting and Wing Development of the Silkworm Bombyx mori
title_full The Role of Chitooligosaccharidolytic β-N-Acetylglucosamindase in the Molting and Wing Development of the Silkworm Bombyx mori
title_fullStr The Role of Chitooligosaccharidolytic β-N-Acetylglucosamindase in the Molting and Wing Development of the Silkworm Bombyx mori
title_full_unstemmed The Role of Chitooligosaccharidolytic β-N-Acetylglucosamindase in the Molting and Wing Development of the Silkworm Bombyx mori
title_short The Role of Chitooligosaccharidolytic β-N-Acetylglucosamindase in the Molting and Wing Development of the Silkworm Bombyx mori
title_sort role of chitooligosaccharidolytic β-n-acetylglucosamindase in the molting and wing development of the silkworm bombyx mori
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8998872/
https://www.ncbi.nlm.nih.gov/pubmed/35409210
http://dx.doi.org/10.3390/ijms23073850
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