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LPS Response Is Impaired by Urban Fine Particulate Matter

Fine particulate matter (PM(2.5)) is a complex mixture of components with diverse chemical and physical characteristics associated with increased respiratory and cardiovascular diseases mortality. Our study aimed to investigate the effects of exposure to concentrated PM(2.5) on LPS-induced lung inju...

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Autores principales: de Souza Xavier Costa, Natália, Ribeiro Júnior, Gabriel, dos Santos Alemany, Adair Aparecida, Belotti, Luciano, Frota Cavalcante, Marcela, Ribeiro, Susan, Matera Veras, Mariana, Kallás, Esper Georges, Saldiva, Paulo Hilário Nascimento, Dolhnikoff, Marisa, Ferraz da Silva, Luiz Fernando
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8998903/
https://www.ncbi.nlm.nih.gov/pubmed/35409273
http://dx.doi.org/10.3390/ijms23073913
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author de Souza Xavier Costa, Natália
Ribeiro Júnior, Gabriel
dos Santos Alemany, Adair Aparecida
Belotti, Luciano
Frota Cavalcante, Marcela
Ribeiro, Susan
Matera Veras, Mariana
Kallás, Esper Georges
Saldiva, Paulo Hilário Nascimento
Dolhnikoff, Marisa
Ferraz da Silva, Luiz Fernando
author_facet de Souza Xavier Costa, Natália
Ribeiro Júnior, Gabriel
dos Santos Alemany, Adair Aparecida
Belotti, Luciano
Frota Cavalcante, Marcela
Ribeiro, Susan
Matera Veras, Mariana
Kallás, Esper Georges
Saldiva, Paulo Hilário Nascimento
Dolhnikoff, Marisa
Ferraz da Silva, Luiz Fernando
author_sort de Souza Xavier Costa, Natália
collection PubMed
description Fine particulate matter (PM(2.5)) is a complex mixture of components with diverse chemical and physical characteristics associated with increased respiratory and cardiovascular diseases mortality. Our study aimed to investigate the effects of exposure to concentrated PM(2.5) on LPS-induced lung injury onset. BALB/c male mice were exposed to either filtered air or ambient fine PM(2.5) in an ambient particle concentrator for 5 weeks. Then, an acute lung injury was induced with nebulized LPS. The animals were euthanized 24 h after the nebulization to either LPS or saline. Inflammatory cells and cytokines (IL-1β, IL-4, IL-5, IL-6, IL-10, IL-17, TNF) were assessed in the blood, bronchoalveolar lavage fluid (BALF), and lung tissue. In addition, lung morphology was assessed by stereological methods. Our results showed that the PM+LPS group showed histological evidence of injury, leukocytosis with increased neutrophils and macrophages, and a mixed inflammatory response profile, with increased KC, IL-6, IL-1β, IL-4, and IL-17. Our analysis shows that there is an interaction between the LPS nebulization and PM(2.5) exposure, differently modulating the inflammatory response, with a distinct response pattern as compared to LPS or PM(2.5) exposure alone. Further studies are required to explain the mechanism of immune modulation caused by PM(2.5) exposure.
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spelling pubmed-89989032022-04-12 LPS Response Is Impaired by Urban Fine Particulate Matter de Souza Xavier Costa, Natália Ribeiro Júnior, Gabriel dos Santos Alemany, Adair Aparecida Belotti, Luciano Frota Cavalcante, Marcela Ribeiro, Susan Matera Veras, Mariana Kallás, Esper Georges Saldiva, Paulo Hilário Nascimento Dolhnikoff, Marisa Ferraz da Silva, Luiz Fernando Int J Mol Sci Article Fine particulate matter (PM(2.5)) is a complex mixture of components with diverse chemical and physical characteristics associated with increased respiratory and cardiovascular diseases mortality. Our study aimed to investigate the effects of exposure to concentrated PM(2.5) on LPS-induced lung injury onset. BALB/c male mice were exposed to either filtered air or ambient fine PM(2.5) in an ambient particle concentrator for 5 weeks. Then, an acute lung injury was induced with nebulized LPS. The animals were euthanized 24 h after the nebulization to either LPS or saline. Inflammatory cells and cytokines (IL-1β, IL-4, IL-5, IL-6, IL-10, IL-17, TNF) were assessed in the blood, bronchoalveolar lavage fluid (BALF), and lung tissue. In addition, lung morphology was assessed by stereological methods. Our results showed that the PM+LPS group showed histological evidence of injury, leukocytosis with increased neutrophils and macrophages, and a mixed inflammatory response profile, with increased KC, IL-6, IL-1β, IL-4, and IL-17. Our analysis shows that there is an interaction between the LPS nebulization and PM(2.5) exposure, differently modulating the inflammatory response, with a distinct response pattern as compared to LPS or PM(2.5) exposure alone. Further studies are required to explain the mechanism of immune modulation caused by PM(2.5) exposure. MDPI 2022-04-01 /pmc/articles/PMC8998903/ /pubmed/35409273 http://dx.doi.org/10.3390/ijms23073913 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
de Souza Xavier Costa, Natália
Ribeiro Júnior, Gabriel
dos Santos Alemany, Adair Aparecida
Belotti, Luciano
Frota Cavalcante, Marcela
Ribeiro, Susan
Matera Veras, Mariana
Kallás, Esper Georges
Saldiva, Paulo Hilário Nascimento
Dolhnikoff, Marisa
Ferraz da Silva, Luiz Fernando
LPS Response Is Impaired by Urban Fine Particulate Matter
title LPS Response Is Impaired by Urban Fine Particulate Matter
title_full LPS Response Is Impaired by Urban Fine Particulate Matter
title_fullStr LPS Response Is Impaired by Urban Fine Particulate Matter
title_full_unstemmed LPS Response Is Impaired by Urban Fine Particulate Matter
title_short LPS Response Is Impaired by Urban Fine Particulate Matter
title_sort lps response is impaired by urban fine particulate matter
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8998903/
https://www.ncbi.nlm.nih.gov/pubmed/35409273
http://dx.doi.org/10.3390/ijms23073913
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