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Expression of Irisin/FNDC5 in Breast Cancer
Irisin is a myokine formed from fibronectin type III domain-containing protein 5 (FNDC5), which can be found in various cancer tissues. FNDC5 and irisin levels have been poorly studied in the tumor tissues of breast cancer (BC). The aim of this study was to determine the levels of irisin expression...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8998925/ https://www.ncbi.nlm.nih.gov/pubmed/35408891 http://dx.doi.org/10.3390/ijms23073530 |
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author | Cebulski, Kamil Nowińska, Katarzyna Jablońska, Karolina Romanowicz, Hanna Smolarz, Beata Dzięgiel, Piotr Podhorska-Okołów, Marzenna |
author_facet | Cebulski, Kamil Nowińska, Katarzyna Jablońska, Karolina Romanowicz, Hanna Smolarz, Beata Dzięgiel, Piotr Podhorska-Okołów, Marzenna |
author_sort | Cebulski, Kamil |
collection | PubMed |
description | Irisin is a myokine formed from fibronectin type III domain-containing protein 5 (FNDC5), which can be found in various cancer tissues. FNDC5 and irisin levels have been poorly studied in the tumor tissues of breast cancer (BC). The aim of this study was to determine the levels of irisin expression in BC tissues and compare them to clinicopathological factors and Ki-67 and PGC-1α expression levels. Tissue microarrays (TMAs) with 541 BC tissues and 61 samples of non-malignant breast disease (NMBD; control) were used to perform immunohistochemical reactions. FNDC5 gene expression was measured in 40 BC tissue samples, 40 samples from the cancer margin, and 16 NMBD samples. RT-PCR was performed for the detection of FNDC5 gene expression. Higher irisin expression was found in BC patients compared to normal breast tissue. FNDC5/irisin expression was higher in patients without lymph node metastases. Longer overall survival was observed in patients with higher irisin expression levels. FNDC5/irisin expression was increased in BC tissues and its high level was a good prognostic factor for survival in BC patients. |
format | Online Article Text |
id | pubmed-8998925 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-89989252022-04-12 Expression of Irisin/FNDC5 in Breast Cancer Cebulski, Kamil Nowińska, Katarzyna Jablońska, Karolina Romanowicz, Hanna Smolarz, Beata Dzięgiel, Piotr Podhorska-Okołów, Marzenna Int J Mol Sci Article Irisin is a myokine formed from fibronectin type III domain-containing protein 5 (FNDC5), which can be found in various cancer tissues. FNDC5 and irisin levels have been poorly studied in the tumor tissues of breast cancer (BC). The aim of this study was to determine the levels of irisin expression in BC tissues and compare them to clinicopathological factors and Ki-67 and PGC-1α expression levels. Tissue microarrays (TMAs) with 541 BC tissues and 61 samples of non-malignant breast disease (NMBD; control) were used to perform immunohistochemical reactions. FNDC5 gene expression was measured in 40 BC tissue samples, 40 samples from the cancer margin, and 16 NMBD samples. RT-PCR was performed for the detection of FNDC5 gene expression. Higher irisin expression was found in BC patients compared to normal breast tissue. FNDC5/irisin expression was higher in patients without lymph node metastases. Longer overall survival was observed in patients with higher irisin expression levels. FNDC5/irisin expression was increased in BC tissues and its high level was a good prognostic factor for survival in BC patients. MDPI 2022-03-24 /pmc/articles/PMC8998925/ /pubmed/35408891 http://dx.doi.org/10.3390/ijms23073530 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cebulski, Kamil Nowińska, Katarzyna Jablońska, Karolina Romanowicz, Hanna Smolarz, Beata Dzięgiel, Piotr Podhorska-Okołów, Marzenna Expression of Irisin/FNDC5 in Breast Cancer |
title | Expression of Irisin/FNDC5 in Breast Cancer |
title_full | Expression of Irisin/FNDC5 in Breast Cancer |
title_fullStr | Expression of Irisin/FNDC5 in Breast Cancer |
title_full_unstemmed | Expression of Irisin/FNDC5 in Breast Cancer |
title_short | Expression of Irisin/FNDC5 in Breast Cancer |
title_sort | expression of irisin/fndc5 in breast cancer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8998925/ https://www.ncbi.nlm.nih.gov/pubmed/35408891 http://dx.doi.org/10.3390/ijms23073530 |
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