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Expression of Irisin/FNDC5 in Breast Cancer

Irisin is a myokine formed from fibronectin type III domain-containing protein 5 (FNDC5), which can be found in various cancer tissues. FNDC5 and irisin levels have been poorly studied in the tumor tissues of breast cancer (BC). The aim of this study was to determine the levels of irisin expression...

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Autores principales: Cebulski, Kamil, Nowińska, Katarzyna, Jablońska, Karolina, Romanowicz, Hanna, Smolarz, Beata, Dzięgiel, Piotr, Podhorska-Okołów, Marzenna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8998925/
https://www.ncbi.nlm.nih.gov/pubmed/35408891
http://dx.doi.org/10.3390/ijms23073530
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author Cebulski, Kamil
Nowińska, Katarzyna
Jablońska, Karolina
Romanowicz, Hanna
Smolarz, Beata
Dzięgiel, Piotr
Podhorska-Okołów, Marzenna
author_facet Cebulski, Kamil
Nowińska, Katarzyna
Jablońska, Karolina
Romanowicz, Hanna
Smolarz, Beata
Dzięgiel, Piotr
Podhorska-Okołów, Marzenna
author_sort Cebulski, Kamil
collection PubMed
description Irisin is a myokine formed from fibronectin type III domain-containing protein 5 (FNDC5), which can be found in various cancer tissues. FNDC5 and irisin levels have been poorly studied in the tumor tissues of breast cancer (BC). The aim of this study was to determine the levels of irisin expression in BC tissues and compare them to clinicopathological factors and Ki-67 and PGC-1α expression levels. Tissue microarrays (TMAs) with 541 BC tissues and 61 samples of non-malignant breast disease (NMBD; control) were used to perform immunohistochemical reactions. FNDC5 gene expression was measured in 40 BC tissue samples, 40 samples from the cancer margin, and 16 NMBD samples. RT-PCR was performed for the detection of FNDC5 gene expression. Higher irisin expression was found in BC patients compared to normal breast tissue. FNDC5/irisin expression was higher in patients without lymph node metastases. Longer overall survival was observed in patients with higher irisin expression levels. FNDC5/irisin expression was increased in BC tissues and its high level was a good prognostic factor for survival in BC patients.
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spelling pubmed-89989252022-04-12 Expression of Irisin/FNDC5 in Breast Cancer Cebulski, Kamil Nowińska, Katarzyna Jablońska, Karolina Romanowicz, Hanna Smolarz, Beata Dzięgiel, Piotr Podhorska-Okołów, Marzenna Int J Mol Sci Article Irisin is a myokine formed from fibronectin type III domain-containing protein 5 (FNDC5), which can be found in various cancer tissues. FNDC5 and irisin levels have been poorly studied in the tumor tissues of breast cancer (BC). The aim of this study was to determine the levels of irisin expression in BC tissues and compare them to clinicopathological factors and Ki-67 and PGC-1α expression levels. Tissue microarrays (TMAs) with 541 BC tissues and 61 samples of non-malignant breast disease (NMBD; control) were used to perform immunohistochemical reactions. FNDC5 gene expression was measured in 40 BC tissue samples, 40 samples from the cancer margin, and 16 NMBD samples. RT-PCR was performed for the detection of FNDC5 gene expression. Higher irisin expression was found in BC patients compared to normal breast tissue. FNDC5/irisin expression was higher in patients without lymph node metastases. Longer overall survival was observed in patients with higher irisin expression levels. FNDC5/irisin expression was increased in BC tissues and its high level was a good prognostic factor for survival in BC patients. MDPI 2022-03-24 /pmc/articles/PMC8998925/ /pubmed/35408891 http://dx.doi.org/10.3390/ijms23073530 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cebulski, Kamil
Nowińska, Katarzyna
Jablońska, Karolina
Romanowicz, Hanna
Smolarz, Beata
Dzięgiel, Piotr
Podhorska-Okołów, Marzenna
Expression of Irisin/FNDC5 in Breast Cancer
title Expression of Irisin/FNDC5 in Breast Cancer
title_full Expression of Irisin/FNDC5 in Breast Cancer
title_fullStr Expression of Irisin/FNDC5 in Breast Cancer
title_full_unstemmed Expression of Irisin/FNDC5 in Breast Cancer
title_short Expression of Irisin/FNDC5 in Breast Cancer
title_sort expression of irisin/fndc5 in breast cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8998925/
https://www.ncbi.nlm.nih.gov/pubmed/35408891
http://dx.doi.org/10.3390/ijms23073530
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