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Proteomic Profiling of Saliva and Tears in Radiated Head and Neck Cancer Patients as Compared to Primary Sjögren’s Syndrome Patients

Patients with head and neck cancer (HNC) and patients with primary Sjögren’s syndrome (pSS) may exhibit similar symptoms of dry mouth and dry eyes, as a result of radiotherapy (RT) or a consequence of disease progression. To identify the proteins that may serve as promising disease biomarkers, we an...

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Autores principales: Hynne, Håvard, Aqrawi, Lara A., Jensen, Janicke Liaaen, Thiede, Bernd, Palm, Øyvind, Amdal, Cecilie Delphin, Westgaard, Kristine Løken, Herlofson, Bente Brokstad, Utheim, Tor P., Galtung, Hilde Kanli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8998953/
https://www.ncbi.nlm.nih.gov/pubmed/35409074
http://dx.doi.org/10.3390/ijms23073714
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author Hynne, Håvard
Aqrawi, Lara A.
Jensen, Janicke Liaaen
Thiede, Bernd
Palm, Øyvind
Amdal, Cecilie Delphin
Westgaard, Kristine Løken
Herlofson, Bente Brokstad
Utheim, Tor P.
Galtung, Hilde Kanli
author_facet Hynne, Håvard
Aqrawi, Lara A.
Jensen, Janicke Liaaen
Thiede, Bernd
Palm, Øyvind
Amdal, Cecilie Delphin
Westgaard, Kristine Løken
Herlofson, Bente Brokstad
Utheim, Tor P.
Galtung, Hilde Kanli
author_sort Hynne, Håvard
collection PubMed
description Patients with head and neck cancer (HNC) and patients with primary Sjögren’s syndrome (pSS) may exhibit similar symptoms of dry mouth and dry eyes, as a result of radiotherapy (RT) or a consequence of disease progression. To identify the proteins that may serve as promising disease biomarkers, we analysed saliva and tears from 29 radiated HNC patients and 21 healthy controls, and saliva from 14 pSS patients by mass spectrometry-based proteomics. The study revealed several upregulated, and in some instances overlapping, proteins in the two patient groups. Histone H1.4 and neutrophil collagenase were upregulated in whole saliva of both patient groups, while caspase-14, histone H4, and protein S100-A9 were upregulated in HNC saliva only. In HCN tear fluid, the most highly upregulated protein was mucin-like protein 1. These overexpressed proteins in saliva and tears play central roles in inflammation, host cell injury, activation of reactive oxygen species, and tissue repair. In conclusion, the similarities and differences in overexpressed proteins detected in saliva from HNC and pSS patients may contribute to the overall understanding of the different pathophysiological mechanisms inducing dry mouth. Thus, the recurring proteins identified could possibly serve as future promising biomarkers
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spelling pubmed-89989532022-04-12 Proteomic Profiling of Saliva and Tears in Radiated Head and Neck Cancer Patients as Compared to Primary Sjögren’s Syndrome Patients Hynne, Håvard Aqrawi, Lara A. Jensen, Janicke Liaaen Thiede, Bernd Palm, Øyvind Amdal, Cecilie Delphin Westgaard, Kristine Løken Herlofson, Bente Brokstad Utheim, Tor P. Galtung, Hilde Kanli Int J Mol Sci Article Patients with head and neck cancer (HNC) and patients with primary Sjögren’s syndrome (pSS) may exhibit similar symptoms of dry mouth and dry eyes, as a result of radiotherapy (RT) or a consequence of disease progression. To identify the proteins that may serve as promising disease biomarkers, we analysed saliva and tears from 29 radiated HNC patients and 21 healthy controls, and saliva from 14 pSS patients by mass spectrometry-based proteomics. The study revealed several upregulated, and in some instances overlapping, proteins in the two patient groups. Histone H1.4 and neutrophil collagenase were upregulated in whole saliva of both patient groups, while caspase-14, histone H4, and protein S100-A9 were upregulated in HNC saliva only. In HCN tear fluid, the most highly upregulated protein was mucin-like protein 1. These overexpressed proteins in saliva and tears play central roles in inflammation, host cell injury, activation of reactive oxygen species, and tissue repair. In conclusion, the similarities and differences in overexpressed proteins detected in saliva from HNC and pSS patients may contribute to the overall understanding of the different pathophysiological mechanisms inducing dry mouth. Thus, the recurring proteins identified could possibly serve as future promising biomarkers MDPI 2022-03-28 /pmc/articles/PMC8998953/ /pubmed/35409074 http://dx.doi.org/10.3390/ijms23073714 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hynne, Håvard
Aqrawi, Lara A.
Jensen, Janicke Liaaen
Thiede, Bernd
Palm, Øyvind
Amdal, Cecilie Delphin
Westgaard, Kristine Løken
Herlofson, Bente Brokstad
Utheim, Tor P.
Galtung, Hilde Kanli
Proteomic Profiling of Saliva and Tears in Radiated Head and Neck Cancer Patients as Compared to Primary Sjögren’s Syndrome Patients
title Proteomic Profiling of Saliva and Tears in Radiated Head and Neck Cancer Patients as Compared to Primary Sjögren’s Syndrome Patients
title_full Proteomic Profiling of Saliva and Tears in Radiated Head and Neck Cancer Patients as Compared to Primary Sjögren’s Syndrome Patients
title_fullStr Proteomic Profiling of Saliva and Tears in Radiated Head and Neck Cancer Patients as Compared to Primary Sjögren’s Syndrome Patients
title_full_unstemmed Proteomic Profiling of Saliva and Tears in Radiated Head and Neck Cancer Patients as Compared to Primary Sjögren’s Syndrome Patients
title_short Proteomic Profiling of Saliva and Tears in Radiated Head and Neck Cancer Patients as Compared to Primary Sjögren’s Syndrome Patients
title_sort proteomic profiling of saliva and tears in radiated head and neck cancer patients as compared to primary sjögren’s syndrome patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8998953/
https://www.ncbi.nlm.nih.gov/pubmed/35409074
http://dx.doi.org/10.3390/ijms23073714
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