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Cross-Omics Analysis of Fenugreek Supplementation Reveals Beneficial Effects Are Caused by Gut Microbiome Changes Not Mammalian Host Physiology

Herbal remedies are increasing in popularity as treatments for metabolic conditions such as obesity and Type 2 Diabetes. One potential therapeutic option is fenugreek seeds (Trigonella foenum-graecum), which have been used for treating high cholesterol and Type 2 diabetes. A proposed mechanism for t...

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Autores principales: Jones, Katarina A., Richard, Allison J., Salbaum, J. Michael, Newman, Susan, Carmouche, Richard, Webb, Sara, Bruce-Keller, Annadora J., Stephens, Jacqueline M., Campagna, Shawn R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8998956/
https://www.ncbi.nlm.nih.gov/pubmed/35409014
http://dx.doi.org/10.3390/ijms23073654
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author Jones, Katarina A.
Richard, Allison J.
Salbaum, J. Michael
Newman, Susan
Carmouche, Richard
Webb, Sara
Bruce-Keller, Annadora J.
Stephens, Jacqueline M.
Campagna, Shawn R.
author_facet Jones, Katarina A.
Richard, Allison J.
Salbaum, J. Michael
Newman, Susan
Carmouche, Richard
Webb, Sara
Bruce-Keller, Annadora J.
Stephens, Jacqueline M.
Campagna, Shawn R.
author_sort Jones, Katarina A.
collection PubMed
description Herbal remedies are increasing in popularity as treatments for metabolic conditions such as obesity and Type 2 Diabetes. One potential therapeutic option is fenugreek seeds (Trigonella foenum-graecum), which have been used for treating high cholesterol and Type 2 diabetes. A proposed mechanism for these benefits is through alterations in the microbiome, which impact mammalian host metabolic function. This study used untargeted metabolomics to investigate the fenugreek-induced alterations in the intestinal, liver, and serum profiles of mice fed either a 60% high-fat or low-fat control diet each with or without fenugreek supplementation (2% w/w) for 14 weeks. Metagenomic analyses of intestinal contents found significant alterations in the relative composition of the gut microbiome resulting from fenugreek supplementation. Specifically, Verrucomicrobia, a phylum containing beneficial bacteria which are correlated with health benefits, increased in relative abundance with fenugreek. Metabolomics partial least squares discriminant analysis revealed substantial fenugreek-induced changes in the large intestines. However, it was observed that while the magnitude of changes was less, significant modifications were present in the liver tissues resulting from fenugreek supplementation. Further analyses revealed metabolic processes affected by fenugreek and showed broad ranging impacts in multiple pathways, including carnitine biosynthesis, cholesterol and bile acid metabolism, and arginine biosynthesis. These pathways may play important roles in the beneficial effects of fenugreek.
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spelling pubmed-89989562022-04-12 Cross-Omics Analysis of Fenugreek Supplementation Reveals Beneficial Effects Are Caused by Gut Microbiome Changes Not Mammalian Host Physiology Jones, Katarina A. Richard, Allison J. Salbaum, J. Michael Newman, Susan Carmouche, Richard Webb, Sara Bruce-Keller, Annadora J. Stephens, Jacqueline M. Campagna, Shawn R. Int J Mol Sci Article Herbal remedies are increasing in popularity as treatments for metabolic conditions such as obesity and Type 2 Diabetes. One potential therapeutic option is fenugreek seeds (Trigonella foenum-graecum), which have been used for treating high cholesterol and Type 2 diabetes. A proposed mechanism for these benefits is through alterations in the microbiome, which impact mammalian host metabolic function. This study used untargeted metabolomics to investigate the fenugreek-induced alterations in the intestinal, liver, and serum profiles of mice fed either a 60% high-fat or low-fat control diet each with or without fenugreek supplementation (2% w/w) for 14 weeks. Metagenomic analyses of intestinal contents found significant alterations in the relative composition of the gut microbiome resulting from fenugreek supplementation. Specifically, Verrucomicrobia, a phylum containing beneficial bacteria which are correlated with health benefits, increased in relative abundance with fenugreek. Metabolomics partial least squares discriminant analysis revealed substantial fenugreek-induced changes in the large intestines. However, it was observed that while the magnitude of changes was less, significant modifications were present in the liver tissues resulting from fenugreek supplementation. Further analyses revealed metabolic processes affected by fenugreek and showed broad ranging impacts in multiple pathways, including carnitine biosynthesis, cholesterol and bile acid metabolism, and arginine biosynthesis. These pathways may play important roles in the beneficial effects of fenugreek. MDPI 2022-03-26 /pmc/articles/PMC8998956/ /pubmed/35409014 http://dx.doi.org/10.3390/ijms23073654 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jones, Katarina A.
Richard, Allison J.
Salbaum, J. Michael
Newman, Susan
Carmouche, Richard
Webb, Sara
Bruce-Keller, Annadora J.
Stephens, Jacqueline M.
Campagna, Shawn R.
Cross-Omics Analysis of Fenugreek Supplementation Reveals Beneficial Effects Are Caused by Gut Microbiome Changes Not Mammalian Host Physiology
title Cross-Omics Analysis of Fenugreek Supplementation Reveals Beneficial Effects Are Caused by Gut Microbiome Changes Not Mammalian Host Physiology
title_full Cross-Omics Analysis of Fenugreek Supplementation Reveals Beneficial Effects Are Caused by Gut Microbiome Changes Not Mammalian Host Physiology
title_fullStr Cross-Omics Analysis of Fenugreek Supplementation Reveals Beneficial Effects Are Caused by Gut Microbiome Changes Not Mammalian Host Physiology
title_full_unstemmed Cross-Omics Analysis of Fenugreek Supplementation Reveals Beneficial Effects Are Caused by Gut Microbiome Changes Not Mammalian Host Physiology
title_short Cross-Omics Analysis of Fenugreek Supplementation Reveals Beneficial Effects Are Caused by Gut Microbiome Changes Not Mammalian Host Physiology
title_sort cross-omics analysis of fenugreek supplementation reveals beneficial effects are caused by gut microbiome changes not mammalian host physiology
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8998956/
https://www.ncbi.nlm.nih.gov/pubmed/35409014
http://dx.doi.org/10.3390/ijms23073654
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