Co(3)O(4) Nanoparticles Uniformly Dispersed in Rational Porous Carbon Nano-Boxes for Significantly Enhanced Electrocatalytic Detection of H(2)O(2) Released from Living Cells

A facile and ingenious method to chemical etching-coordinating a metal-organic framework (MOF) followed by an annealing treatment was proposed to prepare Co(3)O(4) nanoparticles uniformly dispersed in rational porous carbon nano-boxes (Co(3)O(4)@CNBs), which was further used to detect H(2)O(2) released from living cells. The Co(3)O(4)@CNBs H(2)O(2) sensor delivers much higher sensitivity than non-etching/coordinating Co(3)O(4), offering a limit of detection of 2.32 nM. The wide working range covers 10 nM-359 μM H(2)O(2), while possessing good selectivity and excellent reproducibility. Moreover, this biosensor was used to successfully real-time detect H(2)O(2) released from living cells, including both healthy and tumor cells. The excellent performance holds great promise for Co(3)O(4)@CNBs’s applications in electrochemical biomimetic sensing, particularly real-time monitor H(2)O(2) released from living cells.