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Exposure of Buffalo Milkers to Pathogenic Bacteria and Characterization of Isolated Methicillin-Resistant Staphylococcus spp.

The research was focused on the surveillance of the exposure of buffalo milkers in contact with both animals and potentially contaminated equipment, pointing attention on the diffusion of antibiotic-resistant Staphylococcus spp. The monitoring was performed for 12 months, allowing the collection of...

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Autores principales: Carraturo, Federica, Alterisio, Maria Chiara, Guccione, Jacopo, Cerullo, Valeria, Salamone, Michela, Morelli, Michela, Libralato, Giovanni, Russo, Ernesto, d’Angelo, Raffaele, Ciaramella, Paolo, Di Loria, Antonio, Guida, Marco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8999034/
https://www.ncbi.nlm.nih.gov/pubmed/35410035
http://dx.doi.org/10.3390/ijerph19074353
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author Carraturo, Federica
Alterisio, Maria Chiara
Guccione, Jacopo
Cerullo, Valeria
Salamone, Michela
Morelli, Michela
Libralato, Giovanni
Russo, Ernesto
d’Angelo, Raffaele
Ciaramella, Paolo
Di Loria, Antonio
Guida, Marco
author_facet Carraturo, Federica
Alterisio, Maria Chiara
Guccione, Jacopo
Cerullo, Valeria
Salamone, Michela
Morelli, Michela
Libralato, Giovanni
Russo, Ernesto
d’Angelo, Raffaele
Ciaramella, Paolo
Di Loria, Antonio
Guida, Marco
author_sort Carraturo, Federica
collection PubMed
description The research was focused on the surveillance of the exposure of buffalo milkers in contact with both animals and potentially contaminated equipment, pointing attention on the diffusion of antibiotic-resistant Staphylococcus spp. The monitoring was performed for 12 months, allowing the collection of 600 raw milk and buffalo udder surface samples, 192 milking lanes, 400 milking clusters, 160 personal protective equipment (PPEs) and electronic devices surface samples in contact with the workers of four milking parlors located in Southern Italy. The analysis of the milk samples evidenced the highest exposure to the bacteria considered (and mainly to S. aureus) from late winter–spring seasons onward. The possible risk arising from buffalo udder, milking clusters, and lines were instead considered rather stable along the entire period of sampling. The PPEs turned out to be a source of contamination for milkers mainly during the spring and summer periods. The analysis for oxacillin/methicillin resistance revealed in all the farms enrolled an overall amount of 37.5% of Staphylococci strains (belonging to S. aureus, S. haemolyticus, S. pseudintermedius, S. chromogenes species) resistant both to methicillin and oxacillin. The investigation demonstrated that the potential transfer of pathogenic bacteria to humans would have a better chance to occur at milk resumption time (since late winter–spring onward) when the number of animals to be milked is greater and the activity in the milking parlor is more challenging. At the same time, the findings seem to point out that the potential risk may be worsened by a significant presence of oxacillin/methicillin-resistant Staphylococci, potentially resulting from irrational use of antibiotics.
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spelling pubmed-89990342022-04-12 Exposure of Buffalo Milkers to Pathogenic Bacteria and Characterization of Isolated Methicillin-Resistant Staphylococcus spp. Carraturo, Federica Alterisio, Maria Chiara Guccione, Jacopo Cerullo, Valeria Salamone, Michela Morelli, Michela Libralato, Giovanni Russo, Ernesto d’Angelo, Raffaele Ciaramella, Paolo Di Loria, Antonio Guida, Marco Int J Environ Res Public Health Article The research was focused on the surveillance of the exposure of buffalo milkers in contact with both animals and potentially contaminated equipment, pointing attention on the diffusion of antibiotic-resistant Staphylococcus spp. The monitoring was performed for 12 months, allowing the collection of 600 raw milk and buffalo udder surface samples, 192 milking lanes, 400 milking clusters, 160 personal protective equipment (PPEs) and electronic devices surface samples in contact with the workers of four milking parlors located in Southern Italy. The analysis of the milk samples evidenced the highest exposure to the bacteria considered (and mainly to S. aureus) from late winter–spring seasons onward. The possible risk arising from buffalo udder, milking clusters, and lines were instead considered rather stable along the entire period of sampling. The PPEs turned out to be a source of contamination for milkers mainly during the spring and summer periods. The analysis for oxacillin/methicillin resistance revealed in all the farms enrolled an overall amount of 37.5% of Staphylococci strains (belonging to S. aureus, S. haemolyticus, S. pseudintermedius, S. chromogenes species) resistant both to methicillin and oxacillin. The investigation demonstrated that the potential transfer of pathogenic bacteria to humans would have a better chance to occur at milk resumption time (since late winter–spring onward) when the number of animals to be milked is greater and the activity in the milking parlor is more challenging. At the same time, the findings seem to point out that the potential risk may be worsened by a significant presence of oxacillin/methicillin-resistant Staphylococci, potentially resulting from irrational use of antibiotics. MDPI 2022-04-05 /pmc/articles/PMC8999034/ /pubmed/35410035 http://dx.doi.org/10.3390/ijerph19074353 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Carraturo, Federica
Alterisio, Maria Chiara
Guccione, Jacopo
Cerullo, Valeria
Salamone, Michela
Morelli, Michela
Libralato, Giovanni
Russo, Ernesto
d’Angelo, Raffaele
Ciaramella, Paolo
Di Loria, Antonio
Guida, Marco
Exposure of Buffalo Milkers to Pathogenic Bacteria and Characterization of Isolated Methicillin-Resistant Staphylococcus spp.
title Exposure of Buffalo Milkers to Pathogenic Bacteria and Characterization of Isolated Methicillin-Resistant Staphylococcus spp.
title_full Exposure of Buffalo Milkers to Pathogenic Bacteria and Characterization of Isolated Methicillin-Resistant Staphylococcus spp.
title_fullStr Exposure of Buffalo Milkers to Pathogenic Bacteria and Characterization of Isolated Methicillin-Resistant Staphylococcus spp.
title_full_unstemmed Exposure of Buffalo Milkers to Pathogenic Bacteria and Characterization of Isolated Methicillin-Resistant Staphylococcus spp.
title_short Exposure of Buffalo Milkers to Pathogenic Bacteria and Characterization of Isolated Methicillin-Resistant Staphylococcus spp.
title_sort exposure of buffalo milkers to pathogenic bacteria and characterization of isolated methicillin-resistant staphylococcus spp.
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8999034/
https://www.ncbi.nlm.nih.gov/pubmed/35410035
http://dx.doi.org/10.3390/ijerph19074353
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