Cargando…

In Pancreatic Adenocarcinoma Alpha-Synuclein Increases and Marks Peri-Neural Infiltration

α-Synuclein (α-syn) is a protein involved in neuronal degeneration. However, the family of synucleins has recently been demonstrated to be involved in the mechanisms of oncogenesis by selectively accelerating cellular processes leading to cancer. Pancreatic ductal adenocarcinoma (PDAC) is one of the...

Descripción completa

Detalles Bibliográficos
Autores principales: Bianchini, Matteo, Giambelluca, Maria, Scavuzzo, Maria Concetta, Di Franco, Gregorio, Guadagni, Simone, Palmeri, Matteo, Furbetta, Niccolò, Gianardi, Desirée, Costa, Aurelio, Gentiluomo, Manuel, Gaeta, Raffaele, Pollina, Luca Emanuele, Falcone, Alfredo, Vivaldi, Caterina, Di Candio, Giulio, Biagioni, Francesca, Busceti, Carla Letizia, Soldani, Paola, Puglisi-Allegra, Stefano, Morelli, Luca, Fornai, Francesco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8999122/
https://www.ncbi.nlm.nih.gov/pubmed/35409135
http://dx.doi.org/10.3390/ijms23073775
_version_ 1784685115929001984
author Bianchini, Matteo
Giambelluca, Maria
Scavuzzo, Maria Concetta
Di Franco, Gregorio
Guadagni, Simone
Palmeri, Matteo
Furbetta, Niccolò
Gianardi, Desirée
Costa, Aurelio
Gentiluomo, Manuel
Gaeta, Raffaele
Pollina, Luca Emanuele
Falcone, Alfredo
Vivaldi, Caterina
Di Candio, Giulio
Biagioni, Francesca
Busceti, Carla Letizia
Soldani, Paola
Puglisi-Allegra, Stefano
Morelli, Luca
Fornai, Francesco
author_facet Bianchini, Matteo
Giambelluca, Maria
Scavuzzo, Maria Concetta
Di Franco, Gregorio
Guadagni, Simone
Palmeri, Matteo
Furbetta, Niccolò
Gianardi, Desirée
Costa, Aurelio
Gentiluomo, Manuel
Gaeta, Raffaele
Pollina, Luca Emanuele
Falcone, Alfredo
Vivaldi, Caterina
Di Candio, Giulio
Biagioni, Francesca
Busceti, Carla Letizia
Soldani, Paola
Puglisi-Allegra, Stefano
Morelli, Luca
Fornai, Francesco
author_sort Bianchini, Matteo
collection PubMed
description α-Synuclein (α-syn) is a protein involved in neuronal degeneration. However, the family of synucleins has recently been demonstrated to be involved in the mechanisms of oncogenesis by selectively accelerating cellular processes leading to cancer. Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal human cancers, with a specifically high neurotropism. The molecular bases of this biological behavior are currently poorly understood. Here, α-synuclein was analyzed concerning the protein expression in PDAC and the potential association with PDAC neurotropism. Tumor (PDAC) and extra-tumor (extra-PDAC) samples from 20 patients affected by PDAC following pancreatic resections were collected at the General Surgery Unit, University of Pisa. All patients were affected by moderately or poorly differentiated PDAC. The amount of α-syn was compared between tumor and extra-tumor specimen (sampled from non-affected neighboring pancreatic areas) by using in situ immuno-staining with peroxidase anti-α-syn immunohistochemistry, α-syn detection by using Western blotting, and electron microscopy by using α-syn-conjugated immuno-gold particles. All the methods consistently indicate that each PDAC sample possesses a higher amount of α-syn compared with extra-PDAC tissue. Moreover, the expression of α-syn was much higher in those PDAC samples from tumors with perineural infiltration compared with tumors without perineural infiltration.
format Online
Article
Text
id pubmed-8999122
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-89991222022-04-12 In Pancreatic Adenocarcinoma Alpha-Synuclein Increases and Marks Peri-Neural Infiltration Bianchini, Matteo Giambelluca, Maria Scavuzzo, Maria Concetta Di Franco, Gregorio Guadagni, Simone Palmeri, Matteo Furbetta, Niccolò Gianardi, Desirée Costa, Aurelio Gentiluomo, Manuel Gaeta, Raffaele Pollina, Luca Emanuele Falcone, Alfredo Vivaldi, Caterina Di Candio, Giulio Biagioni, Francesca Busceti, Carla Letizia Soldani, Paola Puglisi-Allegra, Stefano Morelli, Luca Fornai, Francesco Int J Mol Sci Article α-Synuclein (α-syn) is a protein involved in neuronal degeneration. However, the family of synucleins has recently been demonstrated to be involved in the mechanisms of oncogenesis by selectively accelerating cellular processes leading to cancer. Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal human cancers, with a specifically high neurotropism. The molecular bases of this biological behavior are currently poorly understood. Here, α-synuclein was analyzed concerning the protein expression in PDAC and the potential association with PDAC neurotropism. Tumor (PDAC) and extra-tumor (extra-PDAC) samples from 20 patients affected by PDAC following pancreatic resections were collected at the General Surgery Unit, University of Pisa. All patients were affected by moderately or poorly differentiated PDAC. The amount of α-syn was compared between tumor and extra-tumor specimen (sampled from non-affected neighboring pancreatic areas) by using in situ immuno-staining with peroxidase anti-α-syn immunohistochemistry, α-syn detection by using Western blotting, and electron microscopy by using α-syn-conjugated immuno-gold particles. All the methods consistently indicate that each PDAC sample possesses a higher amount of α-syn compared with extra-PDAC tissue. Moreover, the expression of α-syn was much higher in those PDAC samples from tumors with perineural infiltration compared with tumors without perineural infiltration. MDPI 2022-03-29 /pmc/articles/PMC8999122/ /pubmed/35409135 http://dx.doi.org/10.3390/ijms23073775 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bianchini, Matteo
Giambelluca, Maria
Scavuzzo, Maria Concetta
Di Franco, Gregorio
Guadagni, Simone
Palmeri, Matteo
Furbetta, Niccolò
Gianardi, Desirée
Costa, Aurelio
Gentiluomo, Manuel
Gaeta, Raffaele
Pollina, Luca Emanuele
Falcone, Alfredo
Vivaldi, Caterina
Di Candio, Giulio
Biagioni, Francesca
Busceti, Carla Letizia
Soldani, Paola
Puglisi-Allegra, Stefano
Morelli, Luca
Fornai, Francesco
In Pancreatic Adenocarcinoma Alpha-Synuclein Increases and Marks Peri-Neural Infiltration
title In Pancreatic Adenocarcinoma Alpha-Synuclein Increases and Marks Peri-Neural Infiltration
title_full In Pancreatic Adenocarcinoma Alpha-Synuclein Increases and Marks Peri-Neural Infiltration
title_fullStr In Pancreatic Adenocarcinoma Alpha-Synuclein Increases and Marks Peri-Neural Infiltration
title_full_unstemmed In Pancreatic Adenocarcinoma Alpha-Synuclein Increases and Marks Peri-Neural Infiltration
title_short In Pancreatic Adenocarcinoma Alpha-Synuclein Increases and Marks Peri-Neural Infiltration
title_sort in pancreatic adenocarcinoma alpha-synuclein increases and marks peri-neural infiltration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8999122/
https://www.ncbi.nlm.nih.gov/pubmed/35409135
http://dx.doi.org/10.3390/ijms23073775
work_keys_str_mv AT bianchinimatteo inpancreaticadenocarcinomaalphasynucleinincreasesandmarksperineuralinfiltration
AT giambellucamaria inpancreaticadenocarcinomaalphasynucleinincreasesandmarksperineuralinfiltration
AT scavuzzomariaconcetta inpancreaticadenocarcinomaalphasynucleinincreasesandmarksperineuralinfiltration
AT difrancogregorio inpancreaticadenocarcinomaalphasynucleinincreasesandmarksperineuralinfiltration
AT guadagnisimone inpancreaticadenocarcinomaalphasynucleinincreasesandmarksperineuralinfiltration
AT palmerimatteo inpancreaticadenocarcinomaalphasynucleinincreasesandmarksperineuralinfiltration
AT furbettaniccolo inpancreaticadenocarcinomaalphasynucleinincreasesandmarksperineuralinfiltration
AT gianardidesiree inpancreaticadenocarcinomaalphasynucleinincreasesandmarksperineuralinfiltration
AT costaaurelio inpancreaticadenocarcinomaalphasynucleinincreasesandmarksperineuralinfiltration
AT gentiluomomanuel inpancreaticadenocarcinomaalphasynucleinincreasesandmarksperineuralinfiltration
AT gaetaraffaele inpancreaticadenocarcinomaalphasynucleinincreasesandmarksperineuralinfiltration
AT pollinalucaemanuele inpancreaticadenocarcinomaalphasynucleinincreasesandmarksperineuralinfiltration
AT falconealfredo inpancreaticadenocarcinomaalphasynucleinincreasesandmarksperineuralinfiltration
AT vivaldicaterina inpancreaticadenocarcinomaalphasynucleinincreasesandmarksperineuralinfiltration
AT dicandiogiulio inpancreaticadenocarcinomaalphasynucleinincreasesandmarksperineuralinfiltration
AT biagionifrancesca inpancreaticadenocarcinomaalphasynucleinincreasesandmarksperineuralinfiltration
AT busceticarlaletizia inpancreaticadenocarcinomaalphasynucleinincreasesandmarksperineuralinfiltration
AT soldanipaola inpancreaticadenocarcinomaalphasynucleinincreasesandmarksperineuralinfiltration
AT puglisiallegrastefano inpancreaticadenocarcinomaalphasynucleinincreasesandmarksperineuralinfiltration
AT morelliluca inpancreaticadenocarcinomaalphasynucleinincreasesandmarksperineuralinfiltration
AT fornaifrancesco inpancreaticadenocarcinomaalphasynucleinincreasesandmarksperineuralinfiltration