Cargando…
Aldehyde Dehydrogenases Expression in Corneal Epithelial Cells with Limbal Stem Cell Deficiency
Purpose: The purpose of the present study is to investigate the expression of aldehyde dehydrogenases (ALDHs) in rabbit corneas with limbal stem cell deficiency (LSCD) and corneas treated with cultured autologous oral mucosa epithelial cell sheet CAOMECS designed to reconstruct the ocular surface wi...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8999523/ https://www.ncbi.nlm.nih.gov/pubmed/35409392 http://dx.doi.org/10.3390/ijms23074032 |
_version_ | 1784685206438936576 |
---|---|
author | Bardag-Gorce, Fawzia Diaz, Alissa Niihara, Robert Stark, Jeremy Cortez, Daileen Lee, Alexander Hoft, Richard Niihara, Yutaka |
author_facet | Bardag-Gorce, Fawzia Diaz, Alissa Niihara, Robert Stark, Jeremy Cortez, Daileen Lee, Alexander Hoft, Richard Niihara, Yutaka |
author_sort | Bardag-Gorce, Fawzia |
collection | PubMed |
description | Purpose: The purpose of the present study is to investigate the expression of aldehyde dehydrogenases (ALDHs) in rabbit corneas with limbal stem cell deficiency (LSCD) and corneas treated with cultured autologous oral mucosa epithelial cell sheet CAOMECS designed to reconstruct the ocular surface with LSCD. Methods: New Zealand white rabbit autologous oral mucosal epithelial cells were isolated from a buccal biopsy and cultured to be grafted back onto corneas of rabbit model of LSCD. Immunofluorescent staining and Western blot analysis were used to compare the expression of ALDH1A1 and ALDH1A3 in healthy, LSCD-diseased, CAOMECS treated corneas. Human oral mucosal and corneal epithelial cells (OMECS and CECs) were cultured and treated with retinoic acid (RA) to further investigate the expression of ALDHs. Results: In healthy corneas, ALDH1A1 and ALDH1A3 were markedly expressed in basal cells of corneal epithelium. In LSCD diseased corneas, ALDH1A1 and ALDH1A3 were markedly expressed in the conjunctivalized apical epithelial cells, the goblet cells, and the stroma. CAOMECS grafted corneas showed a decreased expression of ALDHs as compared to LSCD diseased corneas. Western blot analysis confirmed the up regulation of ALDH1A1 and ALDH1A3 expression in LSCD-diseased corneal epithelial cells. CAOMECS expressed low levels of ALDH1A1 and ALDH1A3, as compared to diseased CECs (D-CEC). When ALDH1A3 was up regulated by retinoic acid treatment in OMECS, Pax-6 expression was down regulated, suggesting a decrease in regenerative capacity when ALDH enzymes are up regulated. Conclusions: These findings report for the first time the up regulation of ALDH1A1 and ALDH1A3 in rabbit corneas with LSCD and document that CAOMECS grafting used to reconstruct corneal epithelium may reduce the expression levels of ALDH enzymes. |
format | Online Article Text |
id | pubmed-8999523 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-89995232022-04-12 Aldehyde Dehydrogenases Expression in Corneal Epithelial Cells with Limbal Stem Cell Deficiency Bardag-Gorce, Fawzia Diaz, Alissa Niihara, Robert Stark, Jeremy Cortez, Daileen Lee, Alexander Hoft, Richard Niihara, Yutaka Int J Mol Sci Article Purpose: The purpose of the present study is to investigate the expression of aldehyde dehydrogenases (ALDHs) in rabbit corneas with limbal stem cell deficiency (LSCD) and corneas treated with cultured autologous oral mucosa epithelial cell sheet CAOMECS designed to reconstruct the ocular surface with LSCD. Methods: New Zealand white rabbit autologous oral mucosal epithelial cells were isolated from a buccal biopsy and cultured to be grafted back onto corneas of rabbit model of LSCD. Immunofluorescent staining and Western blot analysis were used to compare the expression of ALDH1A1 and ALDH1A3 in healthy, LSCD-diseased, CAOMECS treated corneas. Human oral mucosal and corneal epithelial cells (OMECS and CECs) were cultured and treated with retinoic acid (RA) to further investigate the expression of ALDHs. Results: In healthy corneas, ALDH1A1 and ALDH1A3 were markedly expressed in basal cells of corneal epithelium. In LSCD diseased corneas, ALDH1A1 and ALDH1A3 were markedly expressed in the conjunctivalized apical epithelial cells, the goblet cells, and the stroma. CAOMECS grafted corneas showed a decreased expression of ALDHs as compared to LSCD diseased corneas. Western blot analysis confirmed the up regulation of ALDH1A1 and ALDH1A3 expression in LSCD-diseased corneal epithelial cells. CAOMECS expressed low levels of ALDH1A1 and ALDH1A3, as compared to diseased CECs (D-CEC). When ALDH1A3 was up regulated by retinoic acid treatment in OMECS, Pax-6 expression was down regulated, suggesting a decrease in regenerative capacity when ALDH enzymes are up regulated. Conclusions: These findings report for the first time the up regulation of ALDH1A1 and ALDH1A3 in rabbit corneas with LSCD and document that CAOMECS grafting used to reconstruct corneal epithelium may reduce the expression levels of ALDH enzymes. MDPI 2022-04-05 /pmc/articles/PMC8999523/ /pubmed/35409392 http://dx.doi.org/10.3390/ijms23074032 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bardag-Gorce, Fawzia Diaz, Alissa Niihara, Robert Stark, Jeremy Cortez, Daileen Lee, Alexander Hoft, Richard Niihara, Yutaka Aldehyde Dehydrogenases Expression in Corneal Epithelial Cells with Limbal Stem Cell Deficiency |
title | Aldehyde Dehydrogenases Expression in Corneal Epithelial Cells with Limbal Stem Cell Deficiency |
title_full | Aldehyde Dehydrogenases Expression in Corneal Epithelial Cells with Limbal Stem Cell Deficiency |
title_fullStr | Aldehyde Dehydrogenases Expression in Corneal Epithelial Cells with Limbal Stem Cell Deficiency |
title_full_unstemmed | Aldehyde Dehydrogenases Expression in Corneal Epithelial Cells with Limbal Stem Cell Deficiency |
title_short | Aldehyde Dehydrogenases Expression in Corneal Epithelial Cells with Limbal Stem Cell Deficiency |
title_sort | aldehyde dehydrogenases expression in corneal epithelial cells with limbal stem cell deficiency |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8999523/ https://www.ncbi.nlm.nih.gov/pubmed/35409392 http://dx.doi.org/10.3390/ijms23074032 |
work_keys_str_mv | AT bardaggorcefawzia aldehydedehydrogenasesexpressionincornealepithelialcellswithlimbalstemcelldeficiency AT diazalissa aldehydedehydrogenasesexpressionincornealepithelialcellswithlimbalstemcelldeficiency AT niihararobert aldehydedehydrogenasesexpressionincornealepithelialcellswithlimbalstemcelldeficiency AT starkjeremy aldehydedehydrogenasesexpressionincornealepithelialcellswithlimbalstemcelldeficiency AT cortezdaileen aldehydedehydrogenasesexpressionincornealepithelialcellswithlimbalstemcelldeficiency AT leealexander aldehydedehydrogenasesexpressionincornealepithelialcellswithlimbalstemcelldeficiency AT hoftrichard aldehydedehydrogenasesexpressionincornealepithelialcellswithlimbalstemcelldeficiency AT niiharayutaka aldehydedehydrogenasesexpressionincornealepithelialcellswithlimbalstemcelldeficiency |