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Decorin Inhibits Dermal Mesenchymal Cell Migration and Induces Scar Formation

BACKGROUND: Variations in skin healing capacities are observed during different murine embryonic developmental stages. Through embryonic day 16 (E16), embryos are able to regenerate dermal architecture following flank skin wounding; however, after E17, wounds heal incompletely, inducing scar formati...

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Autores principales: Takaya, Kento, Aramaki-Hattori, Noriko, Sakai, Shigeki, Okabe, Keisuke, Asou, Toru, Kishi, Kazuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Lippincott Williams & Wilkins 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9000046/
https://www.ncbi.nlm.nih.gov/pubmed/35425688
http://dx.doi.org/10.1097/GOX.0000000000004245
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author Takaya, Kento
Aramaki-Hattori, Noriko
Sakai, Shigeki
Okabe, Keisuke
Asou, Toru
Kishi, Kazuo
author_facet Takaya, Kento
Aramaki-Hattori, Noriko
Sakai, Shigeki
Okabe, Keisuke
Asou, Toru
Kishi, Kazuo
author_sort Takaya, Kento
collection PubMed
description BACKGROUND: Variations in skin healing capacities are observed during different murine embryonic developmental stages. Through embryonic day 16 (E16), embryos are able to regenerate dermal architecture following flank skin wounding; however, after E17, wounds heal incompletely, inducing scar formation. The regenerative ability of the E16 fetal dermis depends on the migration of dermal mesenchymal cells. Decorin is a small molecule known to affect tissue tensile strength, cell phenotype, and tissue repair, including skin wound healing. In the current study, we evaluated the expression and roles of decorin in wound healing. METHODS: Surgical injury was induced at E16 and E17 in ICR mouse embryos. Decorin expression was evaluated in tissue samples from these embryos using immunohistochemistry and reverse transcription quantitative polymerase chain reaction. Cell migration assays were used to evaluate wound healing capability of separated dermal and fascial tissues. RESULTS: Our results showed that decorin exhibited distinct expression patterns during wound healing at E16 versus E17. Additionally, decorin expression altered cell migration in vitro. Dermal and fascial mesenchymal cells were found to exhibit distinct migration patterns concomitant with altered decorin expression. Specifically, decorin inhibited migration and favored scar formation. CONCLUSION: Decorin expression may contribute to scar formation in the late stage of mouse embryos by inhibiting the migration of dermal mesenchymal cells.
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spelling pubmed-90000462022-04-13 Decorin Inhibits Dermal Mesenchymal Cell Migration and Induces Scar Formation Takaya, Kento Aramaki-Hattori, Noriko Sakai, Shigeki Okabe, Keisuke Asou, Toru Kishi, Kazuo Plast Reconstr Surg Glob Open Research BACKGROUND: Variations in skin healing capacities are observed during different murine embryonic developmental stages. Through embryonic day 16 (E16), embryos are able to regenerate dermal architecture following flank skin wounding; however, after E17, wounds heal incompletely, inducing scar formation. The regenerative ability of the E16 fetal dermis depends on the migration of dermal mesenchymal cells. Decorin is a small molecule known to affect tissue tensile strength, cell phenotype, and tissue repair, including skin wound healing. In the current study, we evaluated the expression and roles of decorin in wound healing. METHODS: Surgical injury was induced at E16 and E17 in ICR mouse embryos. Decorin expression was evaluated in tissue samples from these embryos using immunohistochemistry and reverse transcription quantitative polymerase chain reaction. Cell migration assays were used to evaluate wound healing capability of separated dermal and fascial tissues. RESULTS: Our results showed that decorin exhibited distinct expression patterns during wound healing at E16 versus E17. Additionally, decorin expression altered cell migration in vitro. Dermal and fascial mesenchymal cells were found to exhibit distinct migration patterns concomitant with altered decorin expression. Specifically, decorin inhibited migration and favored scar formation. CONCLUSION: Decorin expression may contribute to scar formation in the late stage of mouse embryos by inhibiting the migration of dermal mesenchymal cells. Lippincott Williams & Wilkins 2022-04-11 /pmc/articles/PMC9000046/ /pubmed/35425688 http://dx.doi.org/10.1097/GOX.0000000000004245 Text en Copyright © 2022 The Authors. Published by Wolters Kluwer Health, Inc. on behalf of The American Society of Plastic Surgeons. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) , where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal.
spellingShingle Research
Takaya, Kento
Aramaki-Hattori, Noriko
Sakai, Shigeki
Okabe, Keisuke
Asou, Toru
Kishi, Kazuo
Decorin Inhibits Dermal Mesenchymal Cell Migration and Induces Scar Formation
title Decorin Inhibits Dermal Mesenchymal Cell Migration and Induces Scar Formation
title_full Decorin Inhibits Dermal Mesenchymal Cell Migration and Induces Scar Formation
title_fullStr Decorin Inhibits Dermal Mesenchymal Cell Migration and Induces Scar Formation
title_full_unstemmed Decorin Inhibits Dermal Mesenchymal Cell Migration and Induces Scar Formation
title_short Decorin Inhibits Dermal Mesenchymal Cell Migration and Induces Scar Formation
title_sort decorin inhibits dermal mesenchymal cell migration and induces scar formation
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9000046/
https://www.ncbi.nlm.nih.gov/pubmed/35425688
http://dx.doi.org/10.1097/GOX.0000000000004245
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