Cargando…

Impact of High-Pressure Homogenization on the Cell Integrity of Tetradesmus obliquus and Seed Germination

Microalgae have almost unlimited applications due to their versatility and robustness to grow in different environmental conditions, their biodiversity and variety of valuable bioactive compounds. Wastewater can be used as a low-cost and readily available medium for microalgae, while the latter remo...

Descripción completa

Detalles Bibliográficos
Autores principales: Ferreira, Alice, Figueiredo, Daniel, Ferreira, Francisca, Ribeiro, Belina, Reis, Alberto, da Silva, Teresa Lopes, Gouveia, Luisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9000265/
https://www.ncbi.nlm.nih.gov/pubmed/35408674
http://dx.doi.org/10.3390/molecules27072275
_version_ 1784685391421374464
author Ferreira, Alice
Figueiredo, Daniel
Ferreira, Francisca
Ribeiro, Belina
Reis, Alberto
da Silva, Teresa Lopes
Gouveia, Luisa
author_facet Ferreira, Alice
Figueiredo, Daniel
Ferreira, Francisca
Ribeiro, Belina
Reis, Alberto
da Silva, Teresa Lopes
Gouveia, Luisa
author_sort Ferreira, Alice
collection PubMed
description Microalgae have almost unlimited applications due to their versatility and robustness to grow in different environmental conditions, their biodiversity and variety of valuable bioactive compounds. Wastewater can be used as a low-cost and readily available medium for microalgae, while the latter removes the pollutants to produce clean water. Nevertheless, since the most valuable metabolites are mainly located inside the microalga cell, their release implies rupturing the cell wall. In this study, Tetradesmus obliquus grown in 5% piggery effluent was disrupted using high-pressure homogenization (HPH). Effects of HPH pressure (100, 300, and 600 bar) and cycles (1, 2 and 3) were tested on the membrane integrity and evaluated using flow cytometry and microscopy. In addition, wheat seed germination trials were carried out using the biomass at different conditions. Increased HPH pressure or number of cycles led to more cell disruption (75% at 600 bar and 3 cycles). However, the highest increase in wheat germination and growth (40–45%) was observed at the lowest pressure (100 bar), where only 46% of the microalga cells were permeabilised, but not disrupted. Non-treated T. obliquus cultures also revealed an enhancing effect on root and shoot length (up to 40%). The filtrate of the initial culture also promoted shoot development compared to water (21%), reinforcing the full use of all the process fractions. Thus, piggery wastewater can be used to produce microalgae biomass, and mild HPH conditions can promote cell permeabilization to release sufficient amounts of bioactive compounds with the ability to enhance plant germination and growth, converting an economic and environmental concern into environmentally sustainable applications.
format Online
Article
Text
id pubmed-9000265
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-90002652022-04-12 Impact of High-Pressure Homogenization on the Cell Integrity of Tetradesmus obliquus and Seed Germination Ferreira, Alice Figueiredo, Daniel Ferreira, Francisca Ribeiro, Belina Reis, Alberto da Silva, Teresa Lopes Gouveia, Luisa Molecules Article Microalgae have almost unlimited applications due to their versatility and robustness to grow in different environmental conditions, their biodiversity and variety of valuable bioactive compounds. Wastewater can be used as a low-cost and readily available medium for microalgae, while the latter removes the pollutants to produce clean water. Nevertheless, since the most valuable metabolites are mainly located inside the microalga cell, their release implies rupturing the cell wall. In this study, Tetradesmus obliquus grown in 5% piggery effluent was disrupted using high-pressure homogenization (HPH). Effects of HPH pressure (100, 300, and 600 bar) and cycles (1, 2 and 3) were tested on the membrane integrity and evaluated using flow cytometry and microscopy. In addition, wheat seed germination trials were carried out using the biomass at different conditions. Increased HPH pressure or number of cycles led to more cell disruption (75% at 600 bar and 3 cycles). However, the highest increase in wheat germination and growth (40–45%) was observed at the lowest pressure (100 bar), where only 46% of the microalga cells were permeabilised, but not disrupted. Non-treated T. obliquus cultures also revealed an enhancing effect on root and shoot length (up to 40%). The filtrate of the initial culture also promoted shoot development compared to water (21%), reinforcing the full use of all the process fractions. Thus, piggery wastewater can be used to produce microalgae biomass, and mild HPH conditions can promote cell permeabilization to release sufficient amounts of bioactive compounds with the ability to enhance plant germination and growth, converting an economic and environmental concern into environmentally sustainable applications. MDPI 2022-03-31 /pmc/articles/PMC9000265/ /pubmed/35408674 http://dx.doi.org/10.3390/molecules27072275 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ferreira, Alice
Figueiredo, Daniel
Ferreira, Francisca
Ribeiro, Belina
Reis, Alberto
da Silva, Teresa Lopes
Gouveia, Luisa
Impact of High-Pressure Homogenization on the Cell Integrity of Tetradesmus obliquus and Seed Germination
title Impact of High-Pressure Homogenization on the Cell Integrity of Tetradesmus obliquus and Seed Germination
title_full Impact of High-Pressure Homogenization on the Cell Integrity of Tetradesmus obliquus and Seed Germination
title_fullStr Impact of High-Pressure Homogenization on the Cell Integrity of Tetradesmus obliquus and Seed Germination
title_full_unstemmed Impact of High-Pressure Homogenization on the Cell Integrity of Tetradesmus obliquus and Seed Germination
title_short Impact of High-Pressure Homogenization on the Cell Integrity of Tetradesmus obliquus and Seed Germination
title_sort impact of high-pressure homogenization on the cell integrity of tetradesmus obliquus and seed germination
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9000265/
https://www.ncbi.nlm.nih.gov/pubmed/35408674
http://dx.doi.org/10.3390/molecules27072275
work_keys_str_mv AT ferreiraalice impactofhighpressurehomogenizationonthecellintegrityoftetradesmusobliquusandseedgermination
AT figueiredodaniel impactofhighpressurehomogenizationonthecellintegrityoftetradesmusobliquusandseedgermination
AT ferreirafrancisca impactofhighpressurehomogenizationonthecellintegrityoftetradesmusobliquusandseedgermination
AT ribeirobelina impactofhighpressurehomogenizationonthecellintegrityoftetradesmusobliquusandseedgermination
AT reisalberto impactofhighpressurehomogenizationonthecellintegrityoftetradesmusobliquusandseedgermination
AT dasilvateresalopes impactofhighpressurehomogenizationonthecellintegrityoftetradesmusobliquusandseedgermination
AT gouveialuisa impactofhighpressurehomogenizationonthecellintegrityoftetradesmusobliquusandseedgermination