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How Structured Metadata Acquisition Contributes to the Reproducibility of Nanosafety Studies: Evaluation by a Round-Robin Test
It has been widely recognized that nanosafety studies are limited in reproducibility, caused by missing or inadequate information and data gaps. Reliable and comprehensive studies should be performed supported by standards or guidelines, which need to be harmonized and usable for the multidisciplina...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9000531/ https://www.ncbi.nlm.nih.gov/pubmed/35407172 http://dx.doi.org/10.3390/nano12071053 |
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author | Elberskirch, Linda Sofranko, Adriana Liebing, Julia Riefler, Norbert Binder, Kunigunde Bonatto Minella, Christian Razum, Matthias Mädler, Lutz Unfried, Klaus Schins, Roel P. F. Kraegeloh, Annette van Thriel, Christoph |
author_facet | Elberskirch, Linda Sofranko, Adriana Liebing, Julia Riefler, Norbert Binder, Kunigunde Bonatto Minella, Christian Razum, Matthias Mädler, Lutz Unfried, Klaus Schins, Roel P. F. Kraegeloh, Annette van Thriel, Christoph |
author_sort | Elberskirch, Linda |
collection | PubMed |
description | It has been widely recognized that nanosafety studies are limited in reproducibility, caused by missing or inadequate information and data gaps. Reliable and comprehensive studies should be performed supported by standards or guidelines, which need to be harmonized and usable for the multidisciplinary field of nanosafety research. The previously described minimal information table (MIT), based on existing standards or guidelines, represents one approach towards harmonization. Here, we demonstrate the applicability and advantages of the MIT by a round-robin test. Its modular structure enables describing individual studies comprehensively by a combination of various relevant aspects. Three laboratories conducted a WST-1 cell viability assay using A549 cells to analyze the effects of the reference nanomaterials NM101 and NM110 according to predefined (S)OPs. The MIT contains relevant and defined descriptive information and quality criteria and thus supported the implementation of the round-robin test from planning, investigation to analysis and data interpretation. As a result, we could identify sources of variability and justify deviating results attributed to differences in specific procedures. Consequently, the use of the MIT contributes to the acquisition of reliable and comprehensive datasets and therefore improves the significance and reusability of nanosafety studies. |
format | Online Article Text |
id | pubmed-9000531 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-90005312022-04-12 How Structured Metadata Acquisition Contributes to the Reproducibility of Nanosafety Studies: Evaluation by a Round-Robin Test Elberskirch, Linda Sofranko, Adriana Liebing, Julia Riefler, Norbert Binder, Kunigunde Bonatto Minella, Christian Razum, Matthias Mädler, Lutz Unfried, Klaus Schins, Roel P. F. Kraegeloh, Annette van Thriel, Christoph Nanomaterials (Basel) Article It has been widely recognized that nanosafety studies are limited in reproducibility, caused by missing or inadequate information and data gaps. Reliable and comprehensive studies should be performed supported by standards or guidelines, which need to be harmonized and usable for the multidisciplinary field of nanosafety research. The previously described minimal information table (MIT), based on existing standards or guidelines, represents one approach towards harmonization. Here, we demonstrate the applicability and advantages of the MIT by a round-robin test. Its modular structure enables describing individual studies comprehensively by a combination of various relevant aspects. Three laboratories conducted a WST-1 cell viability assay using A549 cells to analyze the effects of the reference nanomaterials NM101 and NM110 according to predefined (S)OPs. The MIT contains relevant and defined descriptive information and quality criteria and thus supported the implementation of the round-robin test from planning, investigation to analysis and data interpretation. As a result, we could identify sources of variability and justify deviating results attributed to differences in specific procedures. Consequently, the use of the MIT contributes to the acquisition of reliable and comprehensive datasets and therefore improves the significance and reusability of nanosafety studies. MDPI 2022-03-24 /pmc/articles/PMC9000531/ /pubmed/35407172 http://dx.doi.org/10.3390/nano12071053 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Elberskirch, Linda Sofranko, Adriana Liebing, Julia Riefler, Norbert Binder, Kunigunde Bonatto Minella, Christian Razum, Matthias Mädler, Lutz Unfried, Klaus Schins, Roel P. F. Kraegeloh, Annette van Thriel, Christoph How Structured Metadata Acquisition Contributes to the Reproducibility of Nanosafety Studies: Evaluation by a Round-Robin Test |
title | How Structured Metadata Acquisition Contributes to the Reproducibility of Nanosafety Studies: Evaluation by a Round-Robin Test |
title_full | How Structured Metadata Acquisition Contributes to the Reproducibility of Nanosafety Studies: Evaluation by a Round-Robin Test |
title_fullStr | How Structured Metadata Acquisition Contributes to the Reproducibility of Nanosafety Studies: Evaluation by a Round-Robin Test |
title_full_unstemmed | How Structured Metadata Acquisition Contributes to the Reproducibility of Nanosafety Studies: Evaluation by a Round-Robin Test |
title_short | How Structured Metadata Acquisition Contributes to the Reproducibility of Nanosafety Studies: Evaluation by a Round-Robin Test |
title_sort | how structured metadata acquisition contributes to the reproducibility of nanosafety studies: evaluation by a round-robin test |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9000531/ https://www.ncbi.nlm.nih.gov/pubmed/35407172 http://dx.doi.org/10.3390/nano12071053 |
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