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5-Bromo-3,4-dihydroxybenzaldehyde Promotes Hair Growth through Activation of Wnt/β-Catenin and Autophagy Pathways and Inhibition of TGF-β Pathways in Dermal Papilla Cells

Various studies addressing the increasing problem of hair loss, using natural products with few side effects, have been conducted. 5-bromo-3,4-dihydroxybenzaldehyde (BDB) exhibited anti-inflammatory effects in mouse models of atopic dermatitis and inhibited UVB-induced oxidative stress in keratinocy...

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Autores principales: Kang, Jung-Il, Choi, Youn Kyung, Han, Sang-Chul, Nam, Hyunwoo, Lee, Gilwoo, Kang, Ji-Hoon, Koh, Young Sang, Hyun, Jin Won, Yoo, Eun-Sook, Kang, Hee-Kyoung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9000556/
https://www.ncbi.nlm.nih.gov/pubmed/35408575
http://dx.doi.org/10.3390/molecules27072176
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author Kang, Jung-Il
Choi, Youn Kyung
Han, Sang-Chul
Nam, Hyunwoo
Lee, Gilwoo
Kang, Ji-Hoon
Koh, Young Sang
Hyun, Jin Won
Yoo, Eun-Sook
Kang, Hee-Kyoung
author_facet Kang, Jung-Il
Choi, Youn Kyung
Han, Sang-Chul
Nam, Hyunwoo
Lee, Gilwoo
Kang, Ji-Hoon
Koh, Young Sang
Hyun, Jin Won
Yoo, Eun-Sook
Kang, Hee-Kyoung
author_sort Kang, Jung-Il
collection PubMed
description Various studies addressing the increasing problem of hair loss, using natural products with few side effects, have been conducted. 5-bromo-3,4-dihydroxybenzaldehyde (BDB) exhibited anti-inflammatory effects in mouse models of atopic dermatitis and inhibited UVB-induced oxidative stress in keratinocytes. Here, we investigated its stimulating effect and the underlying mechanism of action on hair growth using rat vibrissa follicles and dermal papilla cells (DPCs), required for the regulation of hair cycle and length. BDB increased the length of hair fibers in rat vibrissa follicles and the proliferation of DPCs, along with causing changes in the levels of cell cycle-related proteins. We investigated whether BDB could trigger anagen-activating signaling pathways, such as the Wnt/β-catenin pathway and autophagy in DPCs. BDB induces activation of the Wnt/β-catenin pathway through the phosphorylation of GSG3β and β-catenin. BDB increased the levels of autophagic vacuoles and autophagy regulatory proteins Atg7, Atg5, Atg16L, and LC3B. We also investigated whether BDB inhibits the TGF-β pathway, which promotes transition to the catagen phase. BDB inhibited the phosphorylation of Smad2 induced by TGF-β1. Thus, BDB can promote hair growth by modulating anagen signaling by activating Wnt/β-catenin and autophagy pathways and inhibiting the TGF-β pathway in DPCs.
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spelling pubmed-90005562022-04-12 5-Bromo-3,4-dihydroxybenzaldehyde Promotes Hair Growth through Activation of Wnt/β-Catenin and Autophagy Pathways and Inhibition of TGF-β Pathways in Dermal Papilla Cells Kang, Jung-Il Choi, Youn Kyung Han, Sang-Chul Nam, Hyunwoo Lee, Gilwoo Kang, Ji-Hoon Koh, Young Sang Hyun, Jin Won Yoo, Eun-Sook Kang, Hee-Kyoung Molecules Article Various studies addressing the increasing problem of hair loss, using natural products with few side effects, have been conducted. 5-bromo-3,4-dihydroxybenzaldehyde (BDB) exhibited anti-inflammatory effects in mouse models of atopic dermatitis and inhibited UVB-induced oxidative stress in keratinocytes. Here, we investigated its stimulating effect and the underlying mechanism of action on hair growth using rat vibrissa follicles and dermal papilla cells (DPCs), required for the regulation of hair cycle and length. BDB increased the length of hair fibers in rat vibrissa follicles and the proliferation of DPCs, along with causing changes in the levels of cell cycle-related proteins. We investigated whether BDB could trigger anagen-activating signaling pathways, such as the Wnt/β-catenin pathway and autophagy in DPCs. BDB induces activation of the Wnt/β-catenin pathway through the phosphorylation of GSG3β and β-catenin. BDB increased the levels of autophagic vacuoles and autophagy regulatory proteins Atg7, Atg5, Atg16L, and LC3B. We also investigated whether BDB inhibits the TGF-β pathway, which promotes transition to the catagen phase. BDB inhibited the phosphorylation of Smad2 induced by TGF-β1. Thus, BDB can promote hair growth by modulating anagen signaling by activating Wnt/β-catenin and autophagy pathways and inhibiting the TGF-β pathway in DPCs. MDPI 2022-03-28 /pmc/articles/PMC9000556/ /pubmed/35408575 http://dx.doi.org/10.3390/molecules27072176 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kang, Jung-Il
Choi, Youn Kyung
Han, Sang-Chul
Nam, Hyunwoo
Lee, Gilwoo
Kang, Ji-Hoon
Koh, Young Sang
Hyun, Jin Won
Yoo, Eun-Sook
Kang, Hee-Kyoung
5-Bromo-3,4-dihydroxybenzaldehyde Promotes Hair Growth through Activation of Wnt/β-Catenin and Autophagy Pathways and Inhibition of TGF-β Pathways in Dermal Papilla Cells
title 5-Bromo-3,4-dihydroxybenzaldehyde Promotes Hair Growth through Activation of Wnt/β-Catenin and Autophagy Pathways and Inhibition of TGF-β Pathways in Dermal Papilla Cells
title_full 5-Bromo-3,4-dihydroxybenzaldehyde Promotes Hair Growth through Activation of Wnt/β-Catenin and Autophagy Pathways and Inhibition of TGF-β Pathways in Dermal Papilla Cells
title_fullStr 5-Bromo-3,4-dihydroxybenzaldehyde Promotes Hair Growth through Activation of Wnt/β-Catenin and Autophagy Pathways and Inhibition of TGF-β Pathways in Dermal Papilla Cells
title_full_unstemmed 5-Bromo-3,4-dihydroxybenzaldehyde Promotes Hair Growth through Activation of Wnt/β-Catenin and Autophagy Pathways and Inhibition of TGF-β Pathways in Dermal Papilla Cells
title_short 5-Bromo-3,4-dihydroxybenzaldehyde Promotes Hair Growth through Activation of Wnt/β-Catenin and Autophagy Pathways and Inhibition of TGF-β Pathways in Dermal Papilla Cells
title_sort 5-bromo-3,4-dihydroxybenzaldehyde promotes hair growth through activation of wnt/β-catenin and autophagy pathways and inhibition of tgf-β pathways in dermal papilla cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9000556/
https://www.ncbi.nlm.nih.gov/pubmed/35408575
http://dx.doi.org/10.3390/molecules27072176
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