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Design of New Concept of Knitted Hernia Implant

A knitted implant, unilaterally modified with plasma-assisted chemical-vapor deposition (PACVD), and with a nano-layer of fluorine derivative supplementation, for reducing the risk of complications related to adhesions, and the formation of a thick postoperative scar was prepared. The biological eva...

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Autores principales: Żywicka, Bogusława, Struszczyk, Marcin Henryk, Paluch, Danuta, Kostanek, Krzysztof, Krucińska, Izabella, Kowalski, Krzysztof, Kopias, Kazimierz, Rybak, Zbigniew, Szymonowicz, Maria, Gutowska, Agnieszka, Kubiak, Paweł
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9000569/
https://www.ncbi.nlm.nih.gov/pubmed/35408005
http://dx.doi.org/10.3390/ma15072671
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author Żywicka, Bogusława
Struszczyk, Marcin Henryk
Paluch, Danuta
Kostanek, Krzysztof
Krucińska, Izabella
Kowalski, Krzysztof
Kopias, Kazimierz
Rybak, Zbigniew
Szymonowicz, Maria
Gutowska, Agnieszka
Kubiak, Paweł
author_facet Żywicka, Bogusława
Struszczyk, Marcin Henryk
Paluch, Danuta
Kostanek, Krzysztof
Krucińska, Izabella
Kowalski, Krzysztof
Kopias, Kazimierz
Rybak, Zbigniew
Szymonowicz, Maria
Gutowska, Agnieszka
Kubiak, Paweł
author_sort Żywicka, Bogusława
collection PubMed
description A knitted implant, unilaterally modified with plasma-assisted chemical-vapor deposition (PACVD), and with a nano-layer of fluorine derivative supplementation, for reducing the risk of complications related to adhesions, and the formation of a thick postoperative scar was prepared. The biological evaluation of designed or modified medical devices is the main aspect of preclinical research. If such studies use a medical device with prolonged contact with connective tissue (more than 30 days), biocompatibility studies require a safety assessment in terms of toxicity in vitro and in vivo, allergenicity, irritation, and cancerogenicity, reproductive and developmental toxicity. The ultimate aspect of biological evaluation is biofunctionality, and evaluation of the local tissue response after implantation, resulting in the determination of all aspects of local biocompatibility with the implemented synthetic material. The implantation of PACVD-modified materials in muscle allows us to estimate the local irritation effect on the connective tissue, determining the risk of scar formation, whereas implantation of the above-mentioned knitted fabric into the abdominal wall, assists with evaluating the risk of fistula formation—the main post-surgical complications. The research aimed to evaluate the local reaction of the soft tissues after the implantation of the knitted implants modified with PACVD of the fluoropolymer in the nanostuctural form. The local effect that occurred during the implantation of the designed implants was quantitatively and qualitatively evaluated when PACVD unmodified (reference), and modified medical devices were implanted in the abdominal cavity (intra-abdominal position) for 12 or into the muscles for 56 weeks. The comparative semi-quantitative histological assessment included the severity of inflammatory cells (multinucleated cells, lymphocytes, plasma cells, macrophages, giant cells) and the tissue response (necrosis, neovascularization, fibrosis, and fat infiltration) on a five-point scale. The knitted implants modified by PACVD did not indicate cumulative tissue response when they were implanted in the muscle and intra-abdominally with direct contact with the viscera. They reduced local tissue reaction (score −2.71 after 56 weeks of the implantation) and internal organ adhesion (irritation score −2.01 and adhesion susceptibility −0.3 after 12 weeks of the implantation) compared with the reference (unmodified by PACVD) knitted implant, which had an identical structure and was made of the same source.
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spelling pubmed-90005692022-04-12 Design of New Concept of Knitted Hernia Implant Żywicka, Bogusława Struszczyk, Marcin Henryk Paluch, Danuta Kostanek, Krzysztof Krucińska, Izabella Kowalski, Krzysztof Kopias, Kazimierz Rybak, Zbigniew Szymonowicz, Maria Gutowska, Agnieszka Kubiak, Paweł Materials (Basel) Article A knitted implant, unilaterally modified with plasma-assisted chemical-vapor deposition (PACVD), and with a nano-layer of fluorine derivative supplementation, for reducing the risk of complications related to adhesions, and the formation of a thick postoperative scar was prepared. The biological evaluation of designed or modified medical devices is the main aspect of preclinical research. If such studies use a medical device with prolonged contact with connective tissue (more than 30 days), biocompatibility studies require a safety assessment in terms of toxicity in vitro and in vivo, allergenicity, irritation, and cancerogenicity, reproductive and developmental toxicity. The ultimate aspect of biological evaluation is biofunctionality, and evaluation of the local tissue response after implantation, resulting in the determination of all aspects of local biocompatibility with the implemented synthetic material. The implantation of PACVD-modified materials in muscle allows us to estimate the local irritation effect on the connective tissue, determining the risk of scar formation, whereas implantation of the above-mentioned knitted fabric into the abdominal wall, assists with evaluating the risk of fistula formation—the main post-surgical complications. The research aimed to evaluate the local reaction of the soft tissues after the implantation of the knitted implants modified with PACVD of the fluoropolymer in the nanostuctural form. The local effect that occurred during the implantation of the designed implants was quantitatively and qualitatively evaluated when PACVD unmodified (reference), and modified medical devices were implanted in the abdominal cavity (intra-abdominal position) for 12 or into the muscles for 56 weeks. The comparative semi-quantitative histological assessment included the severity of inflammatory cells (multinucleated cells, lymphocytes, plasma cells, macrophages, giant cells) and the tissue response (necrosis, neovascularization, fibrosis, and fat infiltration) on a five-point scale. The knitted implants modified by PACVD did not indicate cumulative tissue response when they were implanted in the muscle and intra-abdominally with direct contact with the viscera. They reduced local tissue reaction (score −2.71 after 56 weeks of the implantation) and internal organ adhesion (irritation score −2.01 and adhesion susceptibility −0.3 after 12 weeks of the implantation) compared with the reference (unmodified by PACVD) knitted implant, which had an identical structure and was made of the same source. MDPI 2022-04-05 /pmc/articles/PMC9000569/ /pubmed/35408005 http://dx.doi.org/10.3390/ma15072671 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Żywicka, Bogusława
Struszczyk, Marcin Henryk
Paluch, Danuta
Kostanek, Krzysztof
Krucińska, Izabella
Kowalski, Krzysztof
Kopias, Kazimierz
Rybak, Zbigniew
Szymonowicz, Maria
Gutowska, Agnieszka
Kubiak, Paweł
Design of New Concept of Knitted Hernia Implant
title Design of New Concept of Knitted Hernia Implant
title_full Design of New Concept of Knitted Hernia Implant
title_fullStr Design of New Concept of Knitted Hernia Implant
title_full_unstemmed Design of New Concept of Knitted Hernia Implant
title_short Design of New Concept of Knitted Hernia Implant
title_sort design of new concept of knitted hernia implant
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9000569/
https://www.ncbi.nlm.nih.gov/pubmed/35408005
http://dx.doi.org/10.3390/ma15072671
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