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Effects of the healing activity of rosemary-of-Chapada (Lippia gracilis Schauer) on cutaneous lesions in rats
PURPOSE: To evaluate the effects of rosemary leaf essential oil-based ointments on the healing of rat skin lesions. METHODS: Sixty adult male rats, with dorsal excisional skin wounds made surgically under anesthesia, were divided into three groups (n = 20): Sham group (untreated wounds); control gro...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9000978/ https://www.ncbi.nlm.nih.gov/pubmed/35416859 http://dx.doi.org/10.1590/acb370104 |
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author | Bulhões, Apolônia Agnes Vilar de Carvalho Estevão, Lígia Reis de Moura Florencio-Silva, Rinaldo Simoes, Ricardo Santos Leite, Ana Greice Borba Cunha, Dayana Maria Serafim da Silva Ramos, Clécio Souza Soares, Érica Bruna de Andrade D’Emery, Marcela Barbosa da Câmara, Cláudio Augusto Gomes Evêncio-Neto, Joaquim |
author_facet | Bulhões, Apolônia Agnes Vilar de Carvalho Estevão, Lígia Reis de Moura Florencio-Silva, Rinaldo Simoes, Ricardo Santos Leite, Ana Greice Borba Cunha, Dayana Maria Serafim da Silva Ramos, Clécio Souza Soares, Érica Bruna de Andrade D’Emery, Marcela Barbosa da Câmara, Cláudio Augusto Gomes Evêncio-Neto, Joaquim |
author_sort | Bulhões, Apolônia Agnes Vilar de Carvalho |
collection | PubMed |
description | PURPOSE: To evaluate the effects of rosemary leaf essential oil-based ointments on the healing of rat skin lesions. METHODS: Sixty adult male rats, with dorsal excisional skin wounds made surgically under anesthesia, were divided into three groups (n = 20): Sham group (untreated wounds); control group (CG, wounds treated with vehicle); and essential oil (EO) treated group (wounds treated with essential oil-based ointments), administered topically once daily. Skin wounds were evaluated at 4, 7, 14, and 21 days after EO or vehicle treatments. Lesions were analyzed macroscopically for the contraction degree. Formalin-fixed paraffin-embedded sections of skin wounds were used for histopathological evaluation. RESULTS: Macroscopic evaluation showed wounds edges with thin crust without firmness and yellowish color, along with an improvement in wound contraction in EO group when compared to the other groups. A reduced inflammatory reaction, along with newly formed small diameter capillaries and more organized and elongated collagen fibers, were more frequently observed in EO group than in the other groups. Moreover, blood vessel number and collagen fibers density were significantly higher in EO group. CONCLUSIONS: Skin lesion treatment with rosemary leaf essential oil-based ointments accelerates the initial stages of healing, reduces inflammation, and increases angiogenesis, collagen fibers density, and wound contraction in rats. |
format | Online Article Text |
id | pubmed-9000978 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
record_format | MEDLINE/PubMed |
spelling | pubmed-90009782022-04-22 Effects of the healing activity of rosemary-of-Chapada (Lippia gracilis Schauer) on cutaneous lesions in rats Bulhões, Apolônia Agnes Vilar de Carvalho Estevão, Lígia Reis de Moura Florencio-Silva, Rinaldo Simoes, Ricardo Santos Leite, Ana Greice Borba Cunha, Dayana Maria Serafim da Silva Ramos, Clécio Souza Soares, Érica Bruna de Andrade D’Emery, Marcela Barbosa da Câmara, Cláudio Augusto Gomes Evêncio-Neto, Joaquim Acta Cir Bras Original Article PURPOSE: To evaluate the effects of rosemary leaf essential oil-based ointments on the healing of rat skin lesions. METHODS: Sixty adult male rats, with dorsal excisional skin wounds made surgically under anesthesia, were divided into three groups (n = 20): Sham group (untreated wounds); control group (CG, wounds treated with vehicle); and essential oil (EO) treated group (wounds treated with essential oil-based ointments), administered topically once daily. Skin wounds were evaluated at 4, 7, 14, and 21 days after EO or vehicle treatments. Lesions were analyzed macroscopically for the contraction degree. Formalin-fixed paraffin-embedded sections of skin wounds were used for histopathological evaluation. RESULTS: Macroscopic evaluation showed wounds edges with thin crust without firmness and yellowish color, along with an improvement in wound contraction in EO group when compared to the other groups. A reduced inflammatory reaction, along with newly formed small diameter capillaries and more organized and elongated collagen fibers, were more frequently observed in EO group than in the other groups. Moreover, blood vessel number and collagen fibers density were significantly higher in EO group. CONCLUSIONS: Skin lesion treatment with rosemary leaf essential oil-based ointments accelerates the initial stages of healing, reduces inflammation, and increases angiogenesis, collagen fibers density, and wound contraction in rats. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 2022-04-08 /pmc/articles/PMC9000978/ /pubmed/35416859 http://dx.doi.org/10.1590/acb370104 Text en https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Bulhões, Apolônia Agnes Vilar de Carvalho Estevão, Lígia Reis de Moura Florencio-Silva, Rinaldo Simoes, Ricardo Santos Leite, Ana Greice Borba Cunha, Dayana Maria Serafim da Silva Ramos, Clécio Souza Soares, Érica Bruna de Andrade D’Emery, Marcela Barbosa da Câmara, Cláudio Augusto Gomes Evêncio-Neto, Joaquim Effects of the healing activity of rosemary-of-Chapada (Lippia gracilis Schauer) on cutaneous lesions in rats |
title | Effects of the healing activity of rosemary-of-Chapada (Lippia
gracilis Schauer) on cutaneous lesions in rats |
title_full | Effects of the healing activity of rosemary-of-Chapada (Lippia
gracilis Schauer) on cutaneous lesions in rats |
title_fullStr | Effects of the healing activity of rosemary-of-Chapada (Lippia
gracilis Schauer) on cutaneous lesions in rats |
title_full_unstemmed | Effects of the healing activity of rosemary-of-Chapada (Lippia
gracilis Schauer) on cutaneous lesions in rats |
title_short | Effects of the healing activity of rosemary-of-Chapada (Lippia
gracilis Schauer) on cutaneous lesions in rats |
title_sort | effects of the healing activity of rosemary-of-chapada (lippia
gracilis schauer) on cutaneous lesions in rats |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9000978/ https://www.ncbi.nlm.nih.gov/pubmed/35416859 http://dx.doi.org/10.1590/acb370104 |
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