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Effects of the healing activity of rosemary-of-Chapada (Lippia gracilis Schauer) on cutaneous lesions in rats

PURPOSE: To evaluate the effects of rosemary leaf essential oil-based ointments on the healing of rat skin lesions. METHODS: Sixty adult male rats, with dorsal excisional skin wounds made surgically under anesthesia, were divided into three groups (n = 20): Sham group (untreated wounds); control gro...

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Autores principales: Bulhões, Apolônia Agnes Vilar de Carvalho, Estevão, Lígia Reis de Moura, Florencio-Silva, Rinaldo, Simoes, Ricardo Santos, Leite, Ana Greice Borba, Cunha, Dayana Maria Serafim da Silva, Ramos, Clécio Souza, Soares, Érica Bruna de Andrade, D’Emery, Marcela Barbosa, da Câmara, Cláudio Augusto Gomes, Evêncio-Neto, Joaquim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9000978/
https://www.ncbi.nlm.nih.gov/pubmed/35416859
http://dx.doi.org/10.1590/acb370104
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author Bulhões, Apolônia Agnes Vilar de Carvalho
Estevão, Lígia Reis de Moura
Florencio-Silva, Rinaldo
Simoes, Ricardo Santos
Leite, Ana Greice Borba
Cunha, Dayana Maria Serafim da Silva
Ramos, Clécio Souza
Soares, Érica Bruna de Andrade
D’Emery, Marcela Barbosa
da Câmara, Cláudio Augusto Gomes
Evêncio-Neto, Joaquim
author_facet Bulhões, Apolônia Agnes Vilar de Carvalho
Estevão, Lígia Reis de Moura
Florencio-Silva, Rinaldo
Simoes, Ricardo Santos
Leite, Ana Greice Borba
Cunha, Dayana Maria Serafim da Silva
Ramos, Clécio Souza
Soares, Érica Bruna de Andrade
D’Emery, Marcela Barbosa
da Câmara, Cláudio Augusto Gomes
Evêncio-Neto, Joaquim
author_sort Bulhões, Apolônia Agnes Vilar de Carvalho
collection PubMed
description PURPOSE: To evaluate the effects of rosemary leaf essential oil-based ointments on the healing of rat skin lesions. METHODS: Sixty adult male rats, with dorsal excisional skin wounds made surgically under anesthesia, were divided into three groups (n = 20): Sham group (untreated wounds); control group (CG, wounds treated with vehicle); and essential oil (EO) treated group (wounds treated with essential oil-based ointments), administered topically once daily. Skin wounds were evaluated at 4, 7, 14, and 21 days after EO or vehicle treatments. Lesions were analyzed macroscopically for the contraction degree. Formalin-fixed paraffin-embedded sections of skin wounds were used for histopathological evaluation. RESULTS: Macroscopic evaluation showed wounds edges with thin crust without firmness and yellowish color, along with an improvement in wound contraction in EO group when compared to the other groups. A reduced inflammatory reaction, along with newly formed small diameter capillaries and more organized and elongated collagen fibers, were more frequently observed in EO group than in the other groups. Moreover, blood vessel number and collagen fibers density were significantly higher in EO group. CONCLUSIONS: Skin lesion treatment with rosemary leaf essential oil-based ointments accelerates the initial stages of healing, reduces inflammation, and increases angiogenesis, collagen fibers density, and wound contraction in rats.
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spelling pubmed-90009782022-04-22 Effects of the healing activity of rosemary-of-Chapada (Lippia gracilis Schauer) on cutaneous lesions in rats Bulhões, Apolônia Agnes Vilar de Carvalho Estevão, Lígia Reis de Moura Florencio-Silva, Rinaldo Simoes, Ricardo Santos Leite, Ana Greice Borba Cunha, Dayana Maria Serafim da Silva Ramos, Clécio Souza Soares, Érica Bruna de Andrade D’Emery, Marcela Barbosa da Câmara, Cláudio Augusto Gomes Evêncio-Neto, Joaquim Acta Cir Bras Original Article PURPOSE: To evaluate the effects of rosemary leaf essential oil-based ointments on the healing of rat skin lesions. METHODS: Sixty adult male rats, with dorsal excisional skin wounds made surgically under anesthesia, were divided into three groups (n = 20): Sham group (untreated wounds); control group (CG, wounds treated with vehicle); and essential oil (EO) treated group (wounds treated with essential oil-based ointments), administered topically once daily. Skin wounds were evaluated at 4, 7, 14, and 21 days after EO or vehicle treatments. Lesions were analyzed macroscopically for the contraction degree. Formalin-fixed paraffin-embedded sections of skin wounds were used for histopathological evaluation. RESULTS: Macroscopic evaluation showed wounds edges with thin crust without firmness and yellowish color, along with an improvement in wound contraction in EO group when compared to the other groups. A reduced inflammatory reaction, along with newly formed small diameter capillaries and more organized and elongated collagen fibers, were more frequently observed in EO group than in the other groups. Moreover, blood vessel number and collagen fibers density were significantly higher in EO group. CONCLUSIONS: Skin lesion treatment with rosemary leaf essential oil-based ointments accelerates the initial stages of healing, reduces inflammation, and increases angiogenesis, collagen fibers density, and wound contraction in rats. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 2022-04-08 /pmc/articles/PMC9000978/ /pubmed/35416859 http://dx.doi.org/10.1590/acb370104 Text en https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Bulhões, Apolônia Agnes Vilar de Carvalho
Estevão, Lígia Reis de Moura
Florencio-Silva, Rinaldo
Simoes, Ricardo Santos
Leite, Ana Greice Borba
Cunha, Dayana Maria Serafim da Silva
Ramos, Clécio Souza
Soares, Érica Bruna de Andrade
D’Emery, Marcela Barbosa
da Câmara, Cláudio Augusto Gomes
Evêncio-Neto, Joaquim
Effects of the healing activity of rosemary-of-Chapada (Lippia gracilis Schauer) on cutaneous lesions in rats
title Effects of the healing activity of rosemary-of-Chapada (Lippia gracilis Schauer) on cutaneous lesions in rats
title_full Effects of the healing activity of rosemary-of-Chapada (Lippia gracilis Schauer) on cutaneous lesions in rats
title_fullStr Effects of the healing activity of rosemary-of-Chapada (Lippia gracilis Schauer) on cutaneous lesions in rats
title_full_unstemmed Effects of the healing activity of rosemary-of-Chapada (Lippia gracilis Schauer) on cutaneous lesions in rats
title_short Effects of the healing activity of rosemary-of-Chapada (Lippia gracilis Schauer) on cutaneous lesions in rats
title_sort effects of the healing activity of rosemary-of-chapada (lippia gracilis schauer) on cutaneous lesions in rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9000978/
https://www.ncbi.nlm.nih.gov/pubmed/35416859
http://dx.doi.org/10.1590/acb370104
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