Reprogrammed tracrRNAs enable repurposing of RNAs as crRNAs and sequence-specific RNA biosensors

In type II CRISPR systems, the guide RNA (gRNA) comprises a CRISPR RNA (crRNA) and a hybridized trans-acting CRISPR RNA (tracrRNA), both being essential in guided DNA targeting functions. Although tracrRNAs are diverse in sequence and structure across type II CRISPR systems, the programmability of c...

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Detalles Bibliográficos
Autores principales: Liu, Yang, Pinto, Filipe, Wan, Xinyi, Yang, Zhugen, Peng, Shuguang, Li, Mengxi, Cooper, Jonathan M., Xie, Zhen, French, Christopher E., Wang, Baojun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9001733/
https://www.ncbi.nlm.nih.gov/pubmed/35410423
http://dx.doi.org/10.1038/s41467-022-29604-x

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