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The pre-induction temperature affects recombinant HuGM-CSF aggregation in thermoinducible Escherichia coli

ABSTRACT: The overproduction of recombinant proteins in Escherichia coli leads to insoluble aggregates of proteins called inclusion bodies (IBs). IBs are considered dynamic entities that harbor high percentages of the recombinant protein, which can be found in different conformational states. The pr...

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Detalles Bibliográficos
Autores principales:	Restrepo-Pineda, Sara, Sánchez-Puig, Nuria, Pérez, Néstor O., García‑Hernández, Enrique, Valdez-Cruz, Norma A., Trujillo-Roldán, Mauricio A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Springer Berlin Heidelberg 2022
Materias:	Biotechnological Products and Process Engineering
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9002048/ https://www.ncbi.nlm.nih.gov/pubmed/35412129 http://dx.doi.org/10.1007/s00253-022-11908-z

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