Cargando…

Hydrogen Sulfide Promotes Adventitious Root Development in Cucumber under Salt Stress by Enhancing Antioxidant Ability

As a gas signal molecule, hydrogen sulfide (H(2)S) can enhance plant stress resistance. Here, cucumber (Cucumis sativus ‘Xinchun NO. 4’) explants were used to investigate the role of H(2)S in adventitious root development under salt stress. The results show that sodium chloride (NaCl) at 10 mM produ...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Yayu, Wei, Lijuan, Feng, Li, Zhang, Meiling, Hu, Dongliang, Tie, Jianzhong, Liao, Weibiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9002991/
https://www.ncbi.nlm.nih.gov/pubmed/35406914
http://dx.doi.org/10.3390/plants11070935
_version_ 1784686023973797888
author Liu, Yayu
Wei, Lijuan
Feng, Li
Zhang, Meiling
Hu, Dongliang
Tie, Jianzhong
Liao, Weibiao
author_facet Liu, Yayu
Wei, Lijuan
Feng, Li
Zhang, Meiling
Hu, Dongliang
Tie, Jianzhong
Liao, Weibiao
author_sort Liu, Yayu
collection PubMed
description As a gas signal molecule, hydrogen sulfide (H(2)S) can enhance plant stress resistance. Here, cucumber (Cucumis sativus ‘Xinchun NO. 4’) explants were used to investigate the role of H(2)S in adventitious root development under salt stress. The results show that sodium chloride (NaCl) at 10 mM produced moderate salt stress. The 100 µM sodium hydrosulfide (NaHS) treatment, a H(2)S donor, increased root number and root length by 38.37% and 66.75%, respectively, indicating that H(2)S effectively promoted the occurrence of adventitious roots in cucumber explants under salt stress. The results show that under salt stress, NaHS treatment reduced free proline content and increased the soluble sugar and soluble protein content during rooting. Meanwhile, NaHS treatment enhanced the activities of antioxidant enzymes [peroxidase (POD), superoxide dismutase (SOD), ascorbate peroxidase (APX) and catalase (CAT)], increased the content of ascorbic (ASA) and glutathione (GSH), reduced the content of hydrogen peroxide (H(2)O(2)) and the rate of superoxide radical (O(2−)) production, and decreased relative electrical conductivity (REC) and the content of malondialdehyde (MDA). However, the NaHS scavenger hypotaurine (HT) reversed the above effects of NaHS under salt stress. In summary, H(2)S promoted adventitious root development under salt stress through regulating osmotic substance content and enhancing antioxidant ability in explants.
format Online
Article
Text
id pubmed-9002991
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-90029912022-04-13 Hydrogen Sulfide Promotes Adventitious Root Development in Cucumber under Salt Stress by Enhancing Antioxidant Ability Liu, Yayu Wei, Lijuan Feng, Li Zhang, Meiling Hu, Dongliang Tie, Jianzhong Liao, Weibiao Plants (Basel) Article As a gas signal molecule, hydrogen sulfide (H(2)S) can enhance plant stress resistance. Here, cucumber (Cucumis sativus ‘Xinchun NO. 4’) explants were used to investigate the role of H(2)S in adventitious root development under salt stress. The results show that sodium chloride (NaCl) at 10 mM produced moderate salt stress. The 100 µM sodium hydrosulfide (NaHS) treatment, a H(2)S donor, increased root number and root length by 38.37% and 66.75%, respectively, indicating that H(2)S effectively promoted the occurrence of adventitious roots in cucumber explants under salt stress. The results show that under salt stress, NaHS treatment reduced free proline content and increased the soluble sugar and soluble protein content during rooting. Meanwhile, NaHS treatment enhanced the activities of antioxidant enzymes [peroxidase (POD), superoxide dismutase (SOD), ascorbate peroxidase (APX) and catalase (CAT)], increased the content of ascorbic (ASA) and glutathione (GSH), reduced the content of hydrogen peroxide (H(2)O(2)) and the rate of superoxide radical (O(2−)) production, and decreased relative electrical conductivity (REC) and the content of malondialdehyde (MDA). However, the NaHS scavenger hypotaurine (HT) reversed the above effects of NaHS under salt stress. In summary, H(2)S promoted adventitious root development under salt stress through regulating osmotic substance content and enhancing antioxidant ability in explants. MDPI 2022-03-30 /pmc/articles/PMC9002991/ /pubmed/35406914 http://dx.doi.org/10.3390/plants11070935 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Yayu
Wei, Lijuan
Feng, Li
Zhang, Meiling
Hu, Dongliang
Tie, Jianzhong
Liao, Weibiao
Hydrogen Sulfide Promotes Adventitious Root Development in Cucumber under Salt Stress by Enhancing Antioxidant Ability
title Hydrogen Sulfide Promotes Adventitious Root Development in Cucumber under Salt Stress by Enhancing Antioxidant Ability
title_full Hydrogen Sulfide Promotes Adventitious Root Development in Cucumber under Salt Stress by Enhancing Antioxidant Ability
title_fullStr Hydrogen Sulfide Promotes Adventitious Root Development in Cucumber under Salt Stress by Enhancing Antioxidant Ability
title_full_unstemmed Hydrogen Sulfide Promotes Adventitious Root Development in Cucumber under Salt Stress by Enhancing Antioxidant Ability
title_short Hydrogen Sulfide Promotes Adventitious Root Development in Cucumber under Salt Stress by Enhancing Antioxidant Ability
title_sort hydrogen sulfide promotes adventitious root development in cucumber under salt stress by enhancing antioxidant ability
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9002991/
https://www.ncbi.nlm.nih.gov/pubmed/35406914
http://dx.doi.org/10.3390/plants11070935
work_keys_str_mv AT liuyayu hydrogensulfidepromotesadventitiousrootdevelopmentincucumberundersaltstressbyenhancingantioxidantability
AT weilijuan hydrogensulfidepromotesadventitiousrootdevelopmentincucumberundersaltstressbyenhancingantioxidantability
AT fengli hydrogensulfidepromotesadventitiousrootdevelopmentincucumberundersaltstressbyenhancingantioxidantability
AT zhangmeiling hydrogensulfidepromotesadventitiousrootdevelopmentincucumberundersaltstressbyenhancingantioxidantability
AT hudongliang hydrogensulfidepromotesadventitiousrootdevelopmentincucumberundersaltstressbyenhancingantioxidantability
AT tiejianzhong hydrogensulfidepromotesadventitiousrootdevelopmentincucumberundersaltstressbyenhancingantioxidantability
AT liaoweibiao hydrogensulfidepromotesadventitiousrootdevelopmentincucumberundersaltstressbyenhancingantioxidantability