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Modulation of Chitosan-TPP Nanoparticle Properties for Plasmid DNA Vaccines Delivery
Nucleic acid vaccines have become a revolutionary technology to give a fast, safe, cost-effective and efficient response against viral infections, such as SARS-CoV-2 or Human papillomavirus (HPV). However, to ensure their effectiveness, the development of adequate methods to protect, carry, and deli...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9003200/ https://www.ncbi.nlm.nih.gov/pubmed/35406316 http://dx.doi.org/10.3390/polym14071443 |
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author | Nunes, Renato Serra, Ana Sofia Simaite, Aiva Sousa, Ângela |
author_facet | Nunes, Renato Serra, Ana Sofia Simaite, Aiva Sousa, Ângela |
author_sort | Nunes, Renato |
collection | PubMed |
description | Nucleic acid vaccines have become a revolutionary technology to give a fast, safe, cost-effective and efficient response against viral infections, such as SARS-CoV-2 or Human papillomavirus (HPV). However, to ensure their effectiveness, the development of adequate methods to protect, carry, and deliver nucleic acids is fundamental. In this work, nanoparticles (NPs) of chitosan (CS)-tripolyphosphate (TPP)-plasmid DNA (pDNA) were thoroughly modulated and characterized, by measuring the charge and size through dynamic light scattering (DLS) and morphology by scanning electron microscopy (SEM). Stability, cytotoxicity and cellular uptake of NPs were also evaluated. Finally, the effect of polyplexes on the expression of HPV E7 antigen in human fibroblast and RAW cells was investigated through polymerase chain reaction (PCR) and real-time PCR. The results showed NPs with a spherical/oval shape, narrow size distribution <180 nm and positive zeta potentials (>20 mV) and good stability after one month of storage at 4 °C in formulation buffer or when incubated in culture medium and trypsin. In vitro studies of NPs cytotoxicity revealed that the elimination of formulation buffers led to an improvement in the rate of cell viability. The E7 antigen transcription was also increased for NPs obtained with high pDNA concentration (60 μg/mL). The analyzed CS-TPP-pDNA polyplexes can offer a promising vehicle for nucleic acid vaccines, not only in the prevention or treatment of viral infections, but also to fight emergent and future pathogens. |
format | Online Article Text |
id | pubmed-9003200 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-90032002022-04-13 Modulation of Chitosan-TPP Nanoparticle Properties for Plasmid DNA Vaccines Delivery Nunes, Renato Serra, Ana Sofia Simaite, Aiva Sousa, Ângela Polymers (Basel) Article Nucleic acid vaccines have become a revolutionary technology to give a fast, safe, cost-effective and efficient response against viral infections, such as SARS-CoV-2 or Human papillomavirus (HPV). However, to ensure their effectiveness, the development of adequate methods to protect, carry, and deliver nucleic acids is fundamental. In this work, nanoparticles (NPs) of chitosan (CS)-tripolyphosphate (TPP)-plasmid DNA (pDNA) were thoroughly modulated and characterized, by measuring the charge and size through dynamic light scattering (DLS) and morphology by scanning electron microscopy (SEM). Stability, cytotoxicity and cellular uptake of NPs were also evaluated. Finally, the effect of polyplexes on the expression of HPV E7 antigen in human fibroblast and RAW cells was investigated through polymerase chain reaction (PCR) and real-time PCR. The results showed NPs with a spherical/oval shape, narrow size distribution <180 nm and positive zeta potentials (>20 mV) and good stability after one month of storage at 4 °C in formulation buffer or when incubated in culture medium and trypsin. In vitro studies of NPs cytotoxicity revealed that the elimination of formulation buffers led to an improvement in the rate of cell viability. The E7 antigen transcription was also increased for NPs obtained with high pDNA concentration (60 μg/mL). The analyzed CS-TPP-pDNA polyplexes can offer a promising vehicle for nucleic acid vaccines, not only in the prevention or treatment of viral infections, but also to fight emergent and future pathogens. MDPI 2022-04-01 /pmc/articles/PMC9003200/ /pubmed/35406316 http://dx.doi.org/10.3390/polym14071443 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Nunes, Renato Serra, Ana Sofia Simaite, Aiva Sousa, Ângela Modulation of Chitosan-TPP Nanoparticle Properties for Plasmid DNA Vaccines Delivery |
title | Modulation of Chitosan-TPP Nanoparticle Properties for Plasmid DNA Vaccines Delivery |
title_full | Modulation of Chitosan-TPP Nanoparticle Properties for Plasmid DNA Vaccines Delivery |
title_fullStr | Modulation of Chitosan-TPP Nanoparticle Properties for Plasmid DNA Vaccines Delivery |
title_full_unstemmed | Modulation of Chitosan-TPP Nanoparticle Properties for Plasmid DNA Vaccines Delivery |
title_short | Modulation of Chitosan-TPP Nanoparticle Properties for Plasmid DNA Vaccines Delivery |
title_sort | modulation of chitosan-tpp nanoparticle properties for plasmid dna vaccines delivery |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9003200/ https://www.ncbi.nlm.nih.gov/pubmed/35406316 http://dx.doi.org/10.3390/polym14071443 |
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