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Tri-Layered Vascular Grafts Guide Vascular Cells’ Native-like Arrangement

Bionic grafts hold great promise for directing tissue regeneration. In vascular tissue engineering, although a large number of synthetic grafts have been constructed, these substitutes only partially recapitulated the tri-layered structure of native arteries. Synthetic polymers such as poly(l-lactid...

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Autores principales: Yuan, Xingyu, Li, Wen, Yao, Bin, Li, Zhao, Kong, Deling, Huang, Sha, Zhu, Meifeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9003212/
https://www.ncbi.nlm.nih.gov/pubmed/35406244
http://dx.doi.org/10.3390/polym14071370
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author Yuan, Xingyu
Li, Wen
Yao, Bin
Li, Zhao
Kong, Deling
Huang, Sha
Zhu, Meifeng
author_facet Yuan, Xingyu
Li, Wen
Yao, Bin
Li, Zhao
Kong, Deling
Huang, Sha
Zhu, Meifeng
author_sort Yuan, Xingyu
collection PubMed
description Bionic grafts hold great promise for directing tissue regeneration. In vascular tissue engineering, although a large number of synthetic grafts have been constructed, these substitutes only partially recapitulated the tri-layered structure of native arteries. Synthetic polymers such as poly(l-lactide-co-ε-caprolactone) (PLCL) possess good biocompatibility, controllable degradation, remarkable processability, and sufficient mechanical strength. These properties of PLCL show great promise for fabricating synthetic vascular substitutes. Here, tri-layered PLCL vascular grafts (TVGs) composed of a smooth inner layer, circumferentially aligned fibrous middle layer, and randomly distributed fibrous outer layer were prepared by sequentially using ink printing, wet spinning, and electrospinning techniques. TVGs possessed kink resistance and sufficient mechanical properties (tensile strength, elastic modulus, suture retention strength, and burst pressure) equivalent to the gold standard conduits of clinical application, i.e., human saphenous veins and human internal mammary arteries. The stratified structure of TVGs exhibited a visible guiding effect on specific vascular cells including enhancing endothelial cell (EC) monolayer formation, favoring vascular smooth muscle cells’ (VSMCs) arrangement and elongation, and facilitating fibroblasts’ proliferation and junction establishment. Our research provides a new avenue for designing synthetic vascular grafts with polymers.
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spelling pubmed-90032122022-04-13 Tri-Layered Vascular Grafts Guide Vascular Cells’ Native-like Arrangement Yuan, Xingyu Li, Wen Yao, Bin Li, Zhao Kong, Deling Huang, Sha Zhu, Meifeng Polymers (Basel) Communication Bionic grafts hold great promise for directing tissue regeneration. In vascular tissue engineering, although a large number of synthetic grafts have been constructed, these substitutes only partially recapitulated the tri-layered structure of native arteries. Synthetic polymers such as poly(l-lactide-co-ε-caprolactone) (PLCL) possess good biocompatibility, controllable degradation, remarkable processability, and sufficient mechanical strength. These properties of PLCL show great promise for fabricating synthetic vascular substitutes. Here, tri-layered PLCL vascular grafts (TVGs) composed of a smooth inner layer, circumferentially aligned fibrous middle layer, and randomly distributed fibrous outer layer were prepared by sequentially using ink printing, wet spinning, and electrospinning techniques. TVGs possessed kink resistance and sufficient mechanical properties (tensile strength, elastic modulus, suture retention strength, and burst pressure) equivalent to the gold standard conduits of clinical application, i.e., human saphenous veins and human internal mammary arteries. The stratified structure of TVGs exhibited a visible guiding effect on specific vascular cells including enhancing endothelial cell (EC) monolayer formation, favoring vascular smooth muscle cells’ (VSMCs) arrangement and elongation, and facilitating fibroblasts’ proliferation and junction establishment. Our research provides a new avenue for designing synthetic vascular grafts with polymers. MDPI 2022-03-28 /pmc/articles/PMC9003212/ /pubmed/35406244 http://dx.doi.org/10.3390/polym14071370 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Yuan, Xingyu
Li, Wen
Yao, Bin
Li, Zhao
Kong, Deling
Huang, Sha
Zhu, Meifeng
Tri-Layered Vascular Grafts Guide Vascular Cells’ Native-like Arrangement
title Tri-Layered Vascular Grafts Guide Vascular Cells’ Native-like Arrangement
title_full Tri-Layered Vascular Grafts Guide Vascular Cells’ Native-like Arrangement
title_fullStr Tri-Layered Vascular Grafts Guide Vascular Cells’ Native-like Arrangement
title_full_unstemmed Tri-Layered Vascular Grafts Guide Vascular Cells’ Native-like Arrangement
title_short Tri-Layered Vascular Grafts Guide Vascular Cells’ Native-like Arrangement
title_sort tri-layered vascular grafts guide vascular cells’ native-like arrangement
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9003212/
https://www.ncbi.nlm.nih.gov/pubmed/35406244
http://dx.doi.org/10.3390/polym14071370
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