Cargando…

Whole blood transcriptome profiling identifies candidate genes associated with alopecia in male giant pandas (Ailuropoda melanoleuca)

BACKGROUND: The giant panda (Ailuropoda melanoleuca) is a threatened species endemic to China. Alopecia, characterized by thinning and broken hair, mostly occurs in breeding males. Alopecia significantly affects the health and public image of the giant panda and the cause of alopecia is unclear. RES...

Descripción completa

Detalles Bibliográficos
Autores principales: Shen, Haibo, Li, Caiwu, He, Ming, Huang, Yan, Wang, Jing, Luo, Jing, Wang, Minglei, Yue, Bisong, Zhang, Xiuyue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9004003/
https://www.ncbi.nlm.nih.gov/pubmed/35413801
http://dx.doi.org/10.1186/s12864-022-08501-z
_version_ 1784686198862643200
author Shen, Haibo
Li, Caiwu
He, Ming
Huang, Yan
Wang, Jing
Luo, Jing
Wang, Minglei
Yue, Bisong
Zhang, Xiuyue
author_facet Shen, Haibo
Li, Caiwu
He, Ming
Huang, Yan
Wang, Jing
Luo, Jing
Wang, Minglei
Yue, Bisong
Zhang, Xiuyue
author_sort Shen, Haibo
collection PubMed
description BACKGROUND: The giant panda (Ailuropoda melanoleuca) is a threatened species endemic to China. Alopecia, characterized by thinning and broken hair, mostly occurs in breeding males. Alopecia significantly affects the health and public image of the giant panda and the cause of alopecia is unclear. RESULTS: Here, we researched gene expression profiles of four alopecia giant pandas and seven healthy giant pandas. All pandas were approximately ten years old and their blood samples collected during the breeding season. A total of 458 up-regulated DEGs and 211 down-regulated DEGs were identified. KEGG pathway enrichment identified that upregulated genes were enriched in the Notch signaling pathway and downregulated genes were enriched in ribosome, oxidative phosphorylation, and thermogenesis pathways. We obtained 28 hair growth-related DEGs, and identified three hub genes NOTCH1, SMAD3, and TGFB1 in PPI analysis. Five hair growth-related signaling pathways were identified with abnormal expression, these were Notch, Wnt, TGF-β, Mapk, and PI3K-Akt. The overexpression of NOTCH1 delays inner root sheath differentiation and results in hair shaft abnormalities. The delayed hair regression was associated with a significant decrease in the expression levels of TGFB1. CONCLUSIONS: Our data confirmed the abnormal expression of several hair-related genes and pathways and identified alopecia candidate genes in the giant panda. Results of this study provide theoretical basis for the establishment of prevention and treatment strategies for giant pandas with alopecia. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-022-08501-z.
format Online
Article
Text
id pubmed-9004003
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-90040032022-04-13 Whole blood transcriptome profiling identifies candidate genes associated with alopecia in male giant pandas (Ailuropoda melanoleuca) Shen, Haibo Li, Caiwu He, Ming Huang, Yan Wang, Jing Luo, Jing Wang, Minglei Yue, Bisong Zhang, Xiuyue BMC Genomics Research BACKGROUND: The giant panda (Ailuropoda melanoleuca) is a threatened species endemic to China. Alopecia, characterized by thinning and broken hair, mostly occurs in breeding males. Alopecia significantly affects the health and public image of the giant panda and the cause of alopecia is unclear. RESULTS: Here, we researched gene expression profiles of four alopecia giant pandas and seven healthy giant pandas. All pandas were approximately ten years old and their blood samples collected during the breeding season. A total of 458 up-regulated DEGs and 211 down-regulated DEGs were identified. KEGG pathway enrichment identified that upregulated genes were enriched in the Notch signaling pathway and downregulated genes were enriched in ribosome, oxidative phosphorylation, and thermogenesis pathways. We obtained 28 hair growth-related DEGs, and identified three hub genes NOTCH1, SMAD3, and TGFB1 in PPI analysis. Five hair growth-related signaling pathways were identified with abnormal expression, these were Notch, Wnt, TGF-β, Mapk, and PI3K-Akt. The overexpression of NOTCH1 delays inner root sheath differentiation and results in hair shaft abnormalities. The delayed hair regression was associated with a significant decrease in the expression levels of TGFB1. CONCLUSIONS: Our data confirmed the abnormal expression of several hair-related genes and pathways and identified alopecia candidate genes in the giant panda. Results of this study provide theoretical basis for the establishment of prevention and treatment strategies for giant pandas with alopecia. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-022-08501-z. BioMed Central 2022-04-12 /pmc/articles/PMC9004003/ /pubmed/35413801 http://dx.doi.org/10.1186/s12864-022-08501-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Shen, Haibo
Li, Caiwu
He, Ming
Huang, Yan
Wang, Jing
Luo, Jing
Wang, Minglei
Yue, Bisong
Zhang, Xiuyue
Whole blood transcriptome profiling identifies candidate genes associated with alopecia in male giant pandas (Ailuropoda melanoleuca)
title Whole blood transcriptome profiling identifies candidate genes associated with alopecia in male giant pandas (Ailuropoda melanoleuca)
title_full Whole blood transcriptome profiling identifies candidate genes associated with alopecia in male giant pandas (Ailuropoda melanoleuca)
title_fullStr Whole blood transcriptome profiling identifies candidate genes associated with alopecia in male giant pandas (Ailuropoda melanoleuca)
title_full_unstemmed Whole blood transcriptome profiling identifies candidate genes associated with alopecia in male giant pandas (Ailuropoda melanoleuca)
title_short Whole blood transcriptome profiling identifies candidate genes associated with alopecia in male giant pandas (Ailuropoda melanoleuca)
title_sort whole blood transcriptome profiling identifies candidate genes associated with alopecia in male giant pandas (ailuropoda melanoleuca)
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9004003/
https://www.ncbi.nlm.nih.gov/pubmed/35413801
http://dx.doi.org/10.1186/s12864-022-08501-z
work_keys_str_mv AT shenhaibo wholebloodtranscriptomeprofilingidentifiescandidategenesassociatedwithalopeciainmalegiantpandasailuropodamelanoleuca
AT licaiwu wholebloodtranscriptomeprofilingidentifiescandidategenesassociatedwithalopeciainmalegiantpandasailuropodamelanoleuca
AT heming wholebloodtranscriptomeprofilingidentifiescandidategenesassociatedwithalopeciainmalegiantpandasailuropodamelanoleuca
AT huangyan wholebloodtranscriptomeprofilingidentifiescandidategenesassociatedwithalopeciainmalegiantpandasailuropodamelanoleuca
AT wangjing wholebloodtranscriptomeprofilingidentifiescandidategenesassociatedwithalopeciainmalegiantpandasailuropodamelanoleuca
AT luojing wholebloodtranscriptomeprofilingidentifiescandidategenesassociatedwithalopeciainmalegiantpandasailuropodamelanoleuca
AT wangminglei wholebloodtranscriptomeprofilingidentifiescandidategenesassociatedwithalopeciainmalegiantpandasailuropodamelanoleuca
AT yuebisong wholebloodtranscriptomeprofilingidentifiescandidategenesassociatedwithalopeciainmalegiantpandasailuropodamelanoleuca
AT zhangxiuyue wholebloodtranscriptomeprofilingidentifiescandidategenesassociatedwithalopeciainmalegiantpandasailuropodamelanoleuca