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Gut microbiome signatures linked to HIV-1 reservoir size and viremia control

BACKGROUND: The potential role of the gut microbiome as a predictor of immune-mediated HIV-1 control in the absence of antiretroviral therapy (ART) is still unknown. In the BCN02 clinical trial, which combined the MVA.HIVconsv immunogen with the latency-reversing agent romidepsin in early-ART treate...

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Autores principales: Borgognone, Alessandra, Noguera-Julian, Marc, Oriol, Bruna, Noël-Romas, Laura, Ruiz-Riol, Marta, Guillén, Yolanda, Parera, Mariona, Casadellà, Maria, Duran, Clara, Puertas, Maria C., Català-Moll, Francesc, De Leon, Marlon, Knodel, Samantha, Birse, Kenzie, Manzardo, Christian, Miró, José M., Clotet, Bonaventura, Martinez-Picado, Javier, Moltó, José, Mothe, Beatriz, Burgener, Adam, Brander, Christian, Paredes, Roger
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9004083/
https://www.ncbi.nlm.nih.gov/pubmed/35410461
http://dx.doi.org/10.1186/s40168-022-01247-6
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author Borgognone, Alessandra
Noguera-Julian, Marc
Oriol, Bruna
Noël-Romas, Laura
Ruiz-Riol, Marta
Guillén, Yolanda
Parera, Mariona
Casadellà, Maria
Duran, Clara
Puertas, Maria C.
Català-Moll, Francesc
De Leon, Marlon
Knodel, Samantha
Birse, Kenzie
Manzardo, Christian
Miró, José M.
Clotet, Bonaventura
Martinez-Picado, Javier
Moltó, José
Mothe, Beatriz
Burgener, Adam
Brander, Christian
Paredes, Roger
author_facet Borgognone, Alessandra
Noguera-Julian, Marc
Oriol, Bruna
Noël-Romas, Laura
Ruiz-Riol, Marta
Guillén, Yolanda
Parera, Mariona
Casadellà, Maria
Duran, Clara
Puertas, Maria C.
Català-Moll, Francesc
De Leon, Marlon
Knodel, Samantha
Birse, Kenzie
Manzardo, Christian
Miró, José M.
Clotet, Bonaventura
Martinez-Picado, Javier
Moltó, José
Mothe, Beatriz
Burgener, Adam
Brander, Christian
Paredes, Roger
author_sort Borgognone, Alessandra
collection PubMed
description BACKGROUND: The potential role of the gut microbiome as a predictor of immune-mediated HIV-1 control in the absence of antiretroviral therapy (ART) is still unknown. In the BCN02 clinical trial, which combined the MVA.HIVconsv immunogen with the latency-reversing agent romidepsin in early-ART treated HIV-1 infected individuals, 23% (3/13) of participants showed sustained low-levels of plasma viremia during 32 weeks of a monitored ART pause (MAP). Here, we present a multi-omics analysis to identify compositional and functional gut microbiome patterns associated with HIV-1 control in the BCN02 trial. RESULTS: Viremic controllers during the MAP (controllers) exhibited higher Bacteroidales/Clostridiales ratio and lower microbial gene richness before vaccination and throughout the study intervention when compared to non-controllers. Longitudinal assessment indicated that the gut microbiome of controllers was enriched in pro-inflammatory bacteria and depleted in butyrate-producing bacteria and methanogenic archaea. Functional profiling also showed that metabolic pathways related to fatty acid and lipid biosynthesis were significantly increased in controllers. Fecal metaproteome analyses confirmed that baseline functional differences were mainly driven by Clostridiales. Participants with high baseline Bacteroidales/Clostridiales ratio had increased pre-existing immune activation-related transcripts. The Bacteroidales/Clostridiales ratio as well as host immune-activation signatures inversely correlated with HIV-1 reservoir size. CONCLUSIONS: The present proof-of-concept study suggests the Bacteroidales/Clostridiales ratio as a novel gut microbiome signature associated with HIV-1 reservoir size and immune-mediated viral control after ART interruption. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40168-022-01247-6.
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spelling pubmed-90040832022-04-13 Gut microbiome signatures linked to HIV-1 reservoir size and viremia control Borgognone, Alessandra Noguera-Julian, Marc Oriol, Bruna Noël-Romas, Laura Ruiz-Riol, Marta Guillén, Yolanda Parera, Mariona Casadellà, Maria Duran, Clara Puertas, Maria C. Català-Moll, Francesc De Leon, Marlon Knodel, Samantha Birse, Kenzie Manzardo, Christian Miró, José M. Clotet, Bonaventura Martinez-Picado, Javier Moltó, José Mothe, Beatriz Burgener, Adam Brander, Christian Paredes, Roger Microbiome Research BACKGROUND: The potential role of the gut microbiome as a predictor of immune-mediated HIV-1 control in the absence of antiretroviral therapy (ART) is still unknown. In the BCN02 clinical trial, which combined the MVA.HIVconsv immunogen with the latency-reversing agent romidepsin in early-ART treated HIV-1 infected individuals, 23% (3/13) of participants showed sustained low-levels of plasma viremia during 32 weeks of a monitored ART pause (MAP). Here, we present a multi-omics analysis to identify compositional and functional gut microbiome patterns associated with HIV-1 control in the BCN02 trial. RESULTS: Viremic controllers during the MAP (controllers) exhibited higher Bacteroidales/Clostridiales ratio and lower microbial gene richness before vaccination and throughout the study intervention when compared to non-controllers. Longitudinal assessment indicated that the gut microbiome of controllers was enriched in pro-inflammatory bacteria and depleted in butyrate-producing bacteria and methanogenic archaea. Functional profiling also showed that metabolic pathways related to fatty acid and lipid biosynthesis were significantly increased in controllers. Fecal metaproteome analyses confirmed that baseline functional differences were mainly driven by Clostridiales. Participants with high baseline Bacteroidales/Clostridiales ratio had increased pre-existing immune activation-related transcripts. The Bacteroidales/Clostridiales ratio as well as host immune-activation signatures inversely correlated with HIV-1 reservoir size. CONCLUSIONS: The present proof-of-concept study suggests the Bacteroidales/Clostridiales ratio as a novel gut microbiome signature associated with HIV-1 reservoir size and immune-mediated viral control after ART interruption. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40168-022-01247-6. BioMed Central 2022-04-11 /pmc/articles/PMC9004083/ /pubmed/35410461 http://dx.doi.org/10.1186/s40168-022-01247-6 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Borgognone, Alessandra
Noguera-Julian, Marc
Oriol, Bruna
Noël-Romas, Laura
Ruiz-Riol, Marta
Guillén, Yolanda
Parera, Mariona
Casadellà, Maria
Duran, Clara
Puertas, Maria C.
Català-Moll, Francesc
De Leon, Marlon
Knodel, Samantha
Birse, Kenzie
Manzardo, Christian
Miró, José M.
Clotet, Bonaventura
Martinez-Picado, Javier
Moltó, José
Mothe, Beatriz
Burgener, Adam
Brander, Christian
Paredes, Roger
Gut microbiome signatures linked to HIV-1 reservoir size and viremia control
title Gut microbiome signatures linked to HIV-1 reservoir size and viremia control
title_full Gut microbiome signatures linked to HIV-1 reservoir size and viremia control
title_fullStr Gut microbiome signatures linked to HIV-1 reservoir size and viremia control
title_full_unstemmed Gut microbiome signatures linked to HIV-1 reservoir size and viremia control
title_short Gut microbiome signatures linked to HIV-1 reservoir size and viremia control
title_sort gut microbiome signatures linked to hiv-1 reservoir size and viremia control
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9004083/
https://www.ncbi.nlm.nih.gov/pubmed/35410461
http://dx.doi.org/10.1186/s40168-022-01247-6
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