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Photobiomodulation reduces inflammation but does not influence the hypoxia-inducible factor-1α in pulp tissue of rats after bleaching
OBJECTIVES: To evaluate the influence of photobiomodulation with infrared laser (IRL) in the rat pulp tissue after bleaching, considering the immunolabeling of interleukin (IL)-23 and hypoxia-inducible factor (HIF)-1α. METHODOLOGY: The right and left molars of forty rats were divided into groups: Co...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Faculdade De Odontologia De Bauru - USP
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9004371/ https://www.ncbi.nlm.nih.gov/pubmed/35416830 http://dx.doi.org/10.1590/1678-7757-2021-0559 |
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author | Silva, Isabela Joane Prado Cintra, Luciano Tavares Angelo Ervolino, Edilson Chaves, Hebertt Gonzaga dos Santos Sivieri-AraúJo, Gustavo Briso, André Luiz Fraga Cosme-Silva, Leopoldo Benetti, Francine |
author_facet | Silva, Isabela Joane Prado Cintra, Luciano Tavares Angelo Ervolino, Edilson Chaves, Hebertt Gonzaga dos Santos Sivieri-AraúJo, Gustavo Briso, André Luiz Fraga Cosme-Silva, Leopoldo Benetti, Francine |
author_sort | Silva, Isabela Joane Prado |
collection | PubMed |
description | OBJECTIVES: To evaluate the influence of photobiomodulation with infrared laser (IRL) in the rat pulp tissue after bleaching, considering the immunolabeling of interleukin (IL)-23 and hypoxia-inducible factor (HIF)-1α. METHODOLOGY: The right and left molars of forty rats were divided into groups: Control – with placebo gel and Bleached – with 35% hydrogen peroxide (H(2)O(2)). Half of the rats received one IRL application on both sides, establishing a split-mouth design, which resulted in 4 groups with 20 hemi-maxillae each: Control, Bleach, IRL, and Bleached-IRL. Rats (n=10) from each group were euthanized, at 2- and 30-days mark, and the pulp tissue was evaluated using inflammation and immunolabeling scores. Wilcoxon and Mann-Whitney statistical tests were performed (p<0.05). RESULTS: At the 2-days mark, the Bleached group had severe inflammation and necrosis in the occlusal thirds of the pulp, and moderate to severe inflammation in cervical third, whereas the Bleached-IRL had mild to moderate inflammation (p<0.05). At the 30-days mark, there was no inflammation, but tertiary dentine formation in the bleached groups. Regarding IL-23, severe immunolabeling was observed in the Bleached group (p<0.05) at the 2-days mark; at the 30-days mark, there was a reduction in immunolabeling, in which the Bleached group had moderate and the Bleached-IRL group had mild immunolabeling (p>0.05). HIF-1α was more evident at the 2-days mark in the Bleached group, without significant difference with the Bleached-IRL (p>0.05). The difference was observed between the bleached and control groups, without immunolabeling (p<0.05); at the 30-days mark, the Bleached group had reduction in HIF-1α immunolabeling, while the Bleached-IRL had an increase; the difference remained between the bleached and the controls groups (p<0.05) CONCLUSION: Photobiomodulation using IRL minimized the inflammation and IL-23 immunolabeling in the pulp tissue of rats after dental bleaching, but did not influence significantly the HIF-1α immunolabeling. |
format | Online Article Text |
id | pubmed-9004371 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Faculdade De Odontologia De Bauru - USP |
record_format | MEDLINE/PubMed |
spelling | pubmed-90043712022-04-15 Photobiomodulation reduces inflammation but does not influence the hypoxia-inducible factor-1α in pulp tissue of rats after bleaching Silva, Isabela Joane Prado Cintra, Luciano Tavares Angelo Ervolino, Edilson Chaves, Hebertt Gonzaga dos Santos Sivieri-AraúJo, Gustavo Briso, André Luiz Fraga Cosme-Silva, Leopoldo Benetti, Francine J Appl Oral Sci Original Article OBJECTIVES: To evaluate the influence of photobiomodulation with infrared laser (IRL) in the rat pulp tissue after bleaching, considering the immunolabeling of interleukin (IL)-23 and hypoxia-inducible factor (HIF)-1α. METHODOLOGY: The right and left molars of forty rats were divided into groups: Control – with placebo gel and Bleached – with 35% hydrogen peroxide (H(2)O(2)). Half of the rats received one IRL application on both sides, establishing a split-mouth design, which resulted in 4 groups with 20 hemi-maxillae each: Control, Bleach, IRL, and Bleached-IRL. Rats (n=10) from each group were euthanized, at 2- and 30-days mark, and the pulp tissue was evaluated using inflammation and immunolabeling scores. Wilcoxon and Mann-Whitney statistical tests were performed (p<0.05). RESULTS: At the 2-days mark, the Bleached group had severe inflammation and necrosis in the occlusal thirds of the pulp, and moderate to severe inflammation in cervical third, whereas the Bleached-IRL had mild to moderate inflammation (p<0.05). At the 30-days mark, there was no inflammation, but tertiary dentine formation in the bleached groups. Regarding IL-23, severe immunolabeling was observed in the Bleached group (p<0.05) at the 2-days mark; at the 30-days mark, there was a reduction in immunolabeling, in which the Bleached group had moderate and the Bleached-IRL group had mild immunolabeling (p>0.05). HIF-1α was more evident at the 2-days mark in the Bleached group, without significant difference with the Bleached-IRL (p>0.05). The difference was observed between the bleached and control groups, without immunolabeling (p<0.05); at the 30-days mark, the Bleached group had reduction in HIF-1α immunolabeling, while the Bleached-IRL had an increase; the difference remained between the bleached and the controls groups (p<0.05) CONCLUSION: Photobiomodulation using IRL minimized the inflammation and IL-23 immunolabeling in the pulp tissue of rats after dental bleaching, but did not influence significantly the HIF-1α immunolabeling. Faculdade De Odontologia De Bauru - USP 2022-04-08 /pmc/articles/PMC9004371/ /pubmed/35416830 http://dx.doi.org/10.1590/1678-7757-2021-0559 Text en https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Silva, Isabela Joane Prado Cintra, Luciano Tavares Angelo Ervolino, Edilson Chaves, Hebertt Gonzaga dos Santos Sivieri-AraúJo, Gustavo Briso, André Luiz Fraga Cosme-Silva, Leopoldo Benetti, Francine Photobiomodulation reduces inflammation but does not influence the hypoxia-inducible factor-1α in pulp tissue of rats after bleaching |
title | Photobiomodulation reduces inflammation but does not influence the hypoxia-inducible factor-1α in pulp tissue of rats after bleaching |
title_full | Photobiomodulation reduces inflammation but does not influence the hypoxia-inducible factor-1α in pulp tissue of rats after bleaching |
title_fullStr | Photobiomodulation reduces inflammation but does not influence the hypoxia-inducible factor-1α in pulp tissue of rats after bleaching |
title_full_unstemmed | Photobiomodulation reduces inflammation but does not influence the hypoxia-inducible factor-1α in pulp tissue of rats after bleaching |
title_short | Photobiomodulation reduces inflammation but does not influence the hypoxia-inducible factor-1α in pulp tissue of rats after bleaching |
title_sort | photobiomodulation reduces inflammation but does not influence the hypoxia-inducible factor-1α in pulp tissue of rats after bleaching |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9004371/ https://www.ncbi.nlm.nih.gov/pubmed/35416830 http://dx.doi.org/10.1590/1678-7757-2021-0559 |
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