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Effects of Three Pesticides on the Earthworm Lumbricus terrestris Gut Microbiota

Earthworms play a vital role in the terrestrial ecosystem functioning and maintenance of soil fertility. However, many pesticides, for example, imidacloprid, benomyl, and metribuzin that are world-widely used in agriculture, may be potentially dangerous to earthworms. At the same time, standard test...

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Autores principales: Astaykina, Angelika, Streletskii, Rostislav, Maslov, Mikhail, Krasnov, George, Gorbatov, Victor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9004718/
https://www.ncbi.nlm.nih.gov/pubmed/35422770
http://dx.doi.org/10.3389/fmicb.2022.853535
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author Astaykina, Angelika
Streletskii, Rostislav
Maslov, Mikhail
Krasnov, George
Gorbatov, Victor
author_facet Astaykina, Angelika
Streletskii, Rostislav
Maslov, Mikhail
Krasnov, George
Gorbatov, Victor
author_sort Astaykina, Angelika
collection PubMed
description Earthworms play a vital role in the terrestrial ecosystem functioning and maintenance of soil fertility. However, many pesticides, for example, imidacloprid, benomyl, and metribuzin that are world-widely used in agriculture, may be potentially dangerous to earthworms. At the same time, standard tests for pesticides acute and chronic toxicity do not reflect all aspects of their negative impact and might not be enough sensitive for effective assessment. In this paper, we studied the effects of non-lethal concentrations of imidacloprid, benomyl, and metribuzin on the gut bacterial community of Lumbricus terrestris using high-throughput sequencing approach. We found that pesticides reduced the total bacterial diversity in the earthworm’s gut even at the recommended application rate. Under the applied pesticides, the structure of the gut prokaryotic community underwent changes in the relative abundance of the phyla Proteobacteria, Actinobacteria, Acidobacteria, Planctomyces, Verrucomicrobia, and Cyanobacteria, as well as the genera Haliangium, Gaiella, Paenisporosarcina, Oryzihumus, Candidatus Udaeobacter, and Aquisphaera. Moreover, the pesticides affected the abundance of Verminephrobacter—the earthworms’ nephridia specific symbionts. In general, the negative impact of pesticides on bacterial biodiversity was significant even under pesticides content, which was much lower than their acute and chronic toxicity values for the earthworms. These results highlighted the fact that the earthworm’s gut microbial community is highly sensitive to soil contamination with pesticides. Therefore, such examination should be considered in the pesticide risk assessment protocols.
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spelling pubmed-90047182022-04-13 Effects of Three Pesticides on the Earthworm Lumbricus terrestris Gut Microbiota Astaykina, Angelika Streletskii, Rostislav Maslov, Mikhail Krasnov, George Gorbatov, Victor Front Microbiol Microbiology Earthworms play a vital role in the terrestrial ecosystem functioning and maintenance of soil fertility. However, many pesticides, for example, imidacloprid, benomyl, and metribuzin that are world-widely used in agriculture, may be potentially dangerous to earthworms. At the same time, standard tests for pesticides acute and chronic toxicity do not reflect all aspects of their negative impact and might not be enough sensitive for effective assessment. In this paper, we studied the effects of non-lethal concentrations of imidacloprid, benomyl, and metribuzin on the gut bacterial community of Lumbricus terrestris using high-throughput sequencing approach. We found that pesticides reduced the total bacterial diversity in the earthworm’s gut even at the recommended application rate. Under the applied pesticides, the structure of the gut prokaryotic community underwent changes in the relative abundance of the phyla Proteobacteria, Actinobacteria, Acidobacteria, Planctomyces, Verrucomicrobia, and Cyanobacteria, as well as the genera Haliangium, Gaiella, Paenisporosarcina, Oryzihumus, Candidatus Udaeobacter, and Aquisphaera. Moreover, the pesticides affected the abundance of Verminephrobacter—the earthworms’ nephridia specific symbionts. In general, the negative impact of pesticides on bacterial biodiversity was significant even under pesticides content, which was much lower than their acute and chronic toxicity values for the earthworms. These results highlighted the fact that the earthworm’s gut microbial community is highly sensitive to soil contamination with pesticides. Therefore, such examination should be considered in the pesticide risk assessment protocols. Frontiers Media S.A. 2022-03-29 /pmc/articles/PMC9004718/ /pubmed/35422770 http://dx.doi.org/10.3389/fmicb.2022.853535 Text en Copyright © 2022 Astaykina, Streletskii, Maslov, Krasnov and Gorbatov. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Astaykina, Angelika
Streletskii, Rostislav
Maslov, Mikhail
Krasnov, George
Gorbatov, Victor
Effects of Three Pesticides on the Earthworm Lumbricus terrestris Gut Microbiota
title Effects of Three Pesticides on the Earthworm Lumbricus terrestris Gut Microbiota
title_full Effects of Three Pesticides on the Earthworm Lumbricus terrestris Gut Microbiota
title_fullStr Effects of Three Pesticides on the Earthworm Lumbricus terrestris Gut Microbiota
title_full_unstemmed Effects of Three Pesticides on the Earthworm Lumbricus terrestris Gut Microbiota
title_short Effects of Three Pesticides on the Earthworm Lumbricus terrestris Gut Microbiota
title_sort effects of three pesticides on the earthworm lumbricus terrestris gut microbiota
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9004718/
https://www.ncbi.nlm.nih.gov/pubmed/35422770
http://dx.doi.org/10.3389/fmicb.2022.853535
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