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Experimental and Theoretical Insights on Chemopreventive Effect of the Liposomal Thymoquinone Against Benzo[a]pyrene-Induced Lung Cancer in Swiss Albino Mice

PURPOSE: Thymoquinone (TQ), a phytoconstituent of Nigella sativa seeds, has been studied extensively in various cancer models. However, TQ’s limited water solubility restricts its therapeutic applicability. Our work aims to prepare the novel formulation of TQ and assess its chemopreventive potential...

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Autores principales: Khan, Arif, Alsahli, Mohammed A, Aljasir, Mohammad A, Maswadeh, Hamzah, Mobark, Mugahid A, Azam, Faizul, Allemailem, Khaled S, Alrumaihi, Faris, Alhumaydhi, Fahad A, Almatroudi, Ahmad A, AlSuhaymi, Naif, Khan, Masood A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9005154/
https://www.ncbi.nlm.nih.gov/pubmed/35422652
http://dx.doi.org/10.2147/JIR.S358632
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author Khan, Arif
Alsahli, Mohammed A
Aljasir, Mohammad A
Maswadeh, Hamzah
Mobark, Mugahid A
Azam, Faizul
Allemailem, Khaled S
Alrumaihi, Faris
Alhumaydhi, Fahad A
Almatroudi, Ahmad A
AlSuhaymi, Naif
Khan, Masood A
author_facet Khan, Arif
Alsahli, Mohammed A
Aljasir, Mohammad A
Maswadeh, Hamzah
Mobark, Mugahid A
Azam, Faizul
Allemailem, Khaled S
Alrumaihi, Faris
Alhumaydhi, Fahad A
Almatroudi, Ahmad A
AlSuhaymi, Naif
Khan, Masood A
author_sort Khan, Arif
collection PubMed
description PURPOSE: Thymoquinone (TQ), a phytoconstituent of Nigella sativa seeds, has been studied extensively in various cancer models. However, TQ’s limited water solubility restricts its therapeutic applicability. Our work aims to prepare the novel formulation of TQ and assess its chemopreventive potential in chemically induced lung cancer animal model. METHODS: The polyethylene glycol coated DOPE/CHEMS incorporating TQ-loaded pH-sensitive liposomes (TQPSL) were prepared and characterized. Mice were exposed to benzo[a]pyrene (BaP) thrice a week for 4 weeks to induce lung cancer. TQPSL was administered three times a week for 21 weeks, starting 2 weeks before the first dose of BaP. RESULTS: The prepared TQPSL revealed 85% entrapment efficiency with 128 nm size and −19.5 mv ζ-potential showing high stability of the formulation. The pretreatment of TQPSL showed the recovery in BaP-modulated relative organ weight of lungs, cancer marker enzymes, and antioxidant enzymes in the serum. The histopathological analysis of the tissues showed that TQPSL protected the malignancy in the lungs. The flow cytometry data revealed the induction of apoptosis and decreased intracellular ROS by TQPSL. Molecular docking was performed to predict the TQ’s affinity for eight possible anticancer drug targets linked to lung cancer etiology. The data assisted to identify the serine/threonine-protein kinase BRAF as the most suitable target of TQ with binding energy −6.8 kcal/mol. CONCLUSION: The current findings demonstrated the potential of TQPSL and its possible therapeutic targets of lung cancer. To our knowledge, this is the first research to outline the development of TQ formulation against lung cancer considering its low solubility as well as pulmonary delivery challenges.
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spelling pubmed-90051542022-04-13 Experimental and Theoretical Insights on Chemopreventive Effect of the Liposomal Thymoquinone Against Benzo[a]pyrene-Induced Lung Cancer in Swiss Albino Mice Khan, Arif Alsahli, Mohammed A Aljasir, Mohammad A Maswadeh, Hamzah Mobark, Mugahid A Azam, Faizul Allemailem, Khaled S Alrumaihi, Faris Alhumaydhi, Fahad A Almatroudi, Ahmad A AlSuhaymi, Naif Khan, Masood A J Inflamm Res Original Research PURPOSE: Thymoquinone (TQ), a phytoconstituent of Nigella sativa seeds, has been studied extensively in various cancer models. However, TQ’s limited water solubility restricts its therapeutic applicability. Our work aims to prepare the novel formulation of TQ and assess its chemopreventive potential in chemically induced lung cancer animal model. METHODS: The polyethylene glycol coated DOPE/CHEMS incorporating TQ-loaded pH-sensitive liposomes (TQPSL) were prepared and characterized. Mice were exposed to benzo[a]pyrene (BaP) thrice a week for 4 weeks to induce lung cancer. TQPSL was administered three times a week for 21 weeks, starting 2 weeks before the first dose of BaP. RESULTS: The prepared TQPSL revealed 85% entrapment efficiency with 128 nm size and −19.5 mv ζ-potential showing high stability of the formulation. The pretreatment of TQPSL showed the recovery in BaP-modulated relative organ weight of lungs, cancer marker enzymes, and antioxidant enzymes in the serum. The histopathological analysis of the tissues showed that TQPSL protected the malignancy in the lungs. The flow cytometry data revealed the induction of apoptosis and decreased intracellular ROS by TQPSL. Molecular docking was performed to predict the TQ’s affinity for eight possible anticancer drug targets linked to lung cancer etiology. The data assisted to identify the serine/threonine-protein kinase BRAF as the most suitable target of TQ with binding energy −6.8 kcal/mol. CONCLUSION: The current findings demonstrated the potential of TQPSL and its possible therapeutic targets of lung cancer. To our knowledge, this is the first research to outline the development of TQ formulation against lung cancer considering its low solubility as well as pulmonary delivery challenges. Dove 2022-04-08 /pmc/articles/PMC9005154/ /pubmed/35422652 http://dx.doi.org/10.2147/JIR.S358632 Text en © 2022 Khan et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Khan, Arif
Alsahli, Mohammed A
Aljasir, Mohammad A
Maswadeh, Hamzah
Mobark, Mugahid A
Azam, Faizul
Allemailem, Khaled S
Alrumaihi, Faris
Alhumaydhi, Fahad A
Almatroudi, Ahmad A
AlSuhaymi, Naif
Khan, Masood A
Experimental and Theoretical Insights on Chemopreventive Effect of the Liposomal Thymoquinone Against Benzo[a]pyrene-Induced Lung Cancer in Swiss Albino Mice
title Experimental and Theoretical Insights on Chemopreventive Effect of the Liposomal Thymoquinone Against Benzo[a]pyrene-Induced Lung Cancer in Swiss Albino Mice
title_full Experimental and Theoretical Insights on Chemopreventive Effect of the Liposomal Thymoquinone Against Benzo[a]pyrene-Induced Lung Cancer in Swiss Albino Mice
title_fullStr Experimental and Theoretical Insights on Chemopreventive Effect of the Liposomal Thymoquinone Against Benzo[a]pyrene-Induced Lung Cancer in Swiss Albino Mice
title_full_unstemmed Experimental and Theoretical Insights on Chemopreventive Effect of the Liposomal Thymoquinone Against Benzo[a]pyrene-Induced Lung Cancer in Swiss Albino Mice
title_short Experimental and Theoretical Insights on Chemopreventive Effect of the Liposomal Thymoquinone Against Benzo[a]pyrene-Induced Lung Cancer in Swiss Albino Mice
title_sort experimental and theoretical insights on chemopreventive effect of the liposomal thymoquinone against benzo[a]pyrene-induced lung cancer in swiss albino mice
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9005154/
https://www.ncbi.nlm.nih.gov/pubmed/35422652
http://dx.doi.org/10.2147/JIR.S358632
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