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Intragenic suppressors of unc-104 ( e1265 ) identify potential roles of the conserved stalk region
UNC-104 and its mammalian ortholog, KIF1A, are microtubule motor proteins required for moving synaptic vesicle precursors from neuronal cell bodies to presynaptic sites. These motor proteins consist of N-terminal motor domain, followed by a neck region, three coiled-coil domains and a FHA domain, an...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Caltech Library
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9005198/ https://www.ncbi.nlm.nih.gov/pubmed/35622471 http://dx.doi.org/10.17912/micropub.biology.000539 |
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author | Byrd, Dana T Pearlman, Julie M Jin, Yishi |
author_facet | Byrd, Dana T Pearlman, Julie M Jin, Yishi |
author_sort | Byrd, Dana T |
collection | PubMed |
description | UNC-104 and its mammalian ortholog, KIF1A, are microtubule motor proteins required for moving synaptic vesicle precursors from neuronal cell bodies to presynaptic sites. These motor proteins consist of N-terminal motor domain, followed by a neck region, three coiled-coil domains and a FHA domain, and a C-terminal PH domain. Between the coiled-coil 3 and the PH domain is a large uncharacterized region called stalk. In C. elegans unc-104 ( e1265 ), a partial loss of function mutant, synaptic vesicles are retained in the cell body and absent from presynaptic sites. unc-104 ( e1265 ) contains amino acid substitution D1497N in the PH domain and the mutant proteins show reduced PI(4,5)P(2) binding. Through genetic suppressor screening, we identified amino acid substitutions in a conserved region of the stalk that cause intragenic suppression of unc-104 ( e1265 ). Currently, little is known about the functions of the stalk region. Our findings imply potential compensatory or antagonistic interaction between the stalk region and the cargo binding PH domain. |
format | Online Article Text |
id | pubmed-9005198 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Caltech Library |
record_format | MEDLINE/PubMed |
spelling | pubmed-90051982022-04-13 Intragenic suppressors of unc-104 ( e1265 ) identify potential roles of the conserved stalk region Byrd, Dana T Pearlman, Julie M Jin, Yishi MicroPubl Biol New Finding UNC-104 and its mammalian ortholog, KIF1A, are microtubule motor proteins required for moving synaptic vesicle precursors from neuronal cell bodies to presynaptic sites. These motor proteins consist of N-terminal motor domain, followed by a neck region, three coiled-coil domains and a FHA domain, and a C-terminal PH domain. Between the coiled-coil 3 and the PH domain is a large uncharacterized region called stalk. In C. elegans unc-104 ( e1265 ), a partial loss of function mutant, synaptic vesicles are retained in the cell body and absent from presynaptic sites. unc-104 ( e1265 ) contains amino acid substitution D1497N in the PH domain and the mutant proteins show reduced PI(4,5)P(2) binding. Through genetic suppressor screening, we identified amino acid substitutions in a conserved region of the stalk that cause intragenic suppression of unc-104 ( e1265 ). Currently, little is known about the functions of the stalk region. Our findings imply potential compensatory or antagonistic interaction between the stalk region and the cargo binding PH domain. Caltech Library 2022-04-06 /pmc/articles/PMC9005198/ /pubmed/35622471 http://dx.doi.org/10.17912/micropub.biology.000539 Text en Copyright: © 2022 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | New Finding Byrd, Dana T Pearlman, Julie M Jin, Yishi Intragenic suppressors of unc-104 ( e1265 ) identify potential roles of the conserved stalk region |
title |
Intragenic suppressors of
unc-104
(
e1265
) identify potential roles of the conserved stalk region
|
title_full |
Intragenic suppressors of
unc-104
(
e1265
) identify potential roles of the conserved stalk region
|
title_fullStr |
Intragenic suppressors of
unc-104
(
e1265
) identify potential roles of the conserved stalk region
|
title_full_unstemmed |
Intragenic suppressors of
unc-104
(
e1265
) identify potential roles of the conserved stalk region
|
title_short |
Intragenic suppressors of
unc-104
(
e1265
) identify potential roles of the conserved stalk region
|
title_sort | intragenic suppressors of
unc-104
(
e1265
) identify potential roles of the conserved stalk region |
topic | New Finding |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9005198/ https://www.ncbi.nlm.nih.gov/pubmed/35622471 http://dx.doi.org/10.17912/micropub.biology.000539 |
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