Inducible and reversible RNA N(6)-methyladenosine editing

RNA modifications, including N(6)-methyladenosine (m(6)A), have been reported to regulate fundamental RNA processes and properties, and directly linked to various human diseases. Methods enabling temporal and transcript/locus-specific editing of specific RNA modifications are essential, but still limited, to dissect the dynamic and context-dependent functions of these epigenetic modifications. Here, we develop a chemically inducible and reversible RNA m(6)A modification editing platform integrating chemically induced proximity (CIP) and CRISPR methods. We show that m(6)A editing can be temporally controlled at specific sites of individual RNA transcripts by the addition or removal of the CIP inducer, abscisic acid (ABA), in the system. By incorporating a photo-caged ABA, a light-controlled version of m(6)A editing platform can be developed. We expect that this platform and strategy can be generally applied to edit other RNA modifications in addition to m(6)A.