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Preventive Supplementation of Omega-3 Reduces Pain and Pro-inflammatory Cytokines in a Mouse Model of Complex Regional Pain Syndrome Type I

Complex regional pain syndrome type I (CRPS-I) is a condition that responds poorly to treatments. The role of omega-3 fatty acids in the treatment of inflammatory disorders is well described in the literature; however, few studies have evaluated its therapeutic benefits in different types of pain. W...

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Autores principales: de Oliveira Galassi, Taynah, Fernandes, Paula Franson, Salgado, Afonso Shiguemi Inoue, Cidral-Filho, Francisco José, Piovezan, Anna Paula, Lüdtke, Daniela Dero, Mack, Josiel Mileno, Weber, Kenneth A., Reed, William R., Bobinski, Franciane, Martins, Daniel F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9005766/
https://www.ncbi.nlm.nih.gov/pubmed/35431823
http://dx.doi.org/10.3389/fnint.2022.840249
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author de Oliveira Galassi, Taynah
Fernandes, Paula Franson
Salgado, Afonso Shiguemi Inoue
Cidral-Filho, Francisco José
Piovezan, Anna Paula
Lüdtke, Daniela Dero
Mack, Josiel Mileno
Weber, Kenneth A.
Reed, William R.
Bobinski, Franciane
Martins, Daniel F.
author_facet de Oliveira Galassi, Taynah
Fernandes, Paula Franson
Salgado, Afonso Shiguemi Inoue
Cidral-Filho, Francisco José
Piovezan, Anna Paula
Lüdtke, Daniela Dero
Mack, Josiel Mileno
Weber, Kenneth A.
Reed, William R.
Bobinski, Franciane
Martins, Daniel F.
author_sort de Oliveira Galassi, Taynah
collection PubMed
description Complex regional pain syndrome type I (CRPS-I) is a condition that responds poorly to treatments. The role of omega-3 fatty acids in the treatment of inflammatory disorders is well described in the literature; however, few studies have evaluated its therapeutic benefits in different types of pain. We evaluated the potential antihyperalgesic and anti-inflammatory effects of preventive omega-3 supplementation in an animal model of CRPS-I. In experiment 1, Swiss female mice were supplemented for 30 days with omega-3 before the induction of the CRPS-I model and 14 days after. Mechanical hyperalgesia was evaluated at baseline and from the 4th to the 14th day after CPRS-I induction along with open field locomotor activity after 30 days of supplementation. In experiment 2, Swiss female mice were supplemented for 30 days with omega-3 and then subjected to the CRPS-I model. Twenty-four hours later the animals were euthanized, and tissue samples of the spinal cord and right posterior paw muscle were taken to measure pro-inflammatory cytokine TNF and IL-1β concentrations. Omega-3 supplementation produced antihyperalgesic and anti-inflammatory effects, as well as reducing pro-inflammatory cytokine concentrations, without altering the animals’ locomotion. No open field locomotor changes were found. The 30-day supplementation at the tested dose was effective in the CRPS-I model.
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spelling pubmed-90057662022-04-14 Preventive Supplementation of Omega-3 Reduces Pain and Pro-inflammatory Cytokines in a Mouse Model of Complex Regional Pain Syndrome Type I de Oliveira Galassi, Taynah Fernandes, Paula Franson Salgado, Afonso Shiguemi Inoue Cidral-Filho, Francisco José Piovezan, Anna Paula Lüdtke, Daniela Dero Mack, Josiel Mileno Weber, Kenneth A. Reed, William R. Bobinski, Franciane Martins, Daniel F. Front Integr Neurosci Neuroscience Complex regional pain syndrome type I (CRPS-I) is a condition that responds poorly to treatments. The role of omega-3 fatty acids in the treatment of inflammatory disorders is well described in the literature; however, few studies have evaluated its therapeutic benefits in different types of pain. We evaluated the potential antihyperalgesic and anti-inflammatory effects of preventive omega-3 supplementation in an animal model of CRPS-I. In experiment 1, Swiss female mice were supplemented for 30 days with omega-3 before the induction of the CRPS-I model and 14 days after. Mechanical hyperalgesia was evaluated at baseline and from the 4th to the 14th day after CPRS-I induction along with open field locomotor activity after 30 days of supplementation. In experiment 2, Swiss female mice were supplemented for 30 days with omega-3 and then subjected to the CRPS-I model. Twenty-four hours later the animals were euthanized, and tissue samples of the spinal cord and right posterior paw muscle were taken to measure pro-inflammatory cytokine TNF and IL-1β concentrations. Omega-3 supplementation produced antihyperalgesic and anti-inflammatory effects, as well as reducing pro-inflammatory cytokine concentrations, without altering the animals’ locomotion. No open field locomotor changes were found. The 30-day supplementation at the tested dose was effective in the CRPS-I model. Frontiers Media S.A. 2022-03-30 /pmc/articles/PMC9005766/ /pubmed/35431823 http://dx.doi.org/10.3389/fnint.2022.840249 Text en Copyright © 2022 de Oliveira Galassi, Fernandes, Salgado, Cidral-Filho, Piovezan, Lüdtke, Mack, Weber, Reed, Bobinski and Martins. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
de Oliveira Galassi, Taynah
Fernandes, Paula Franson
Salgado, Afonso Shiguemi Inoue
Cidral-Filho, Francisco José
Piovezan, Anna Paula
Lüdtke, Daniela Dero
Mack, Josiel Mileno
Weber, Kenneth A.
Reed, William R.
Bobinski, Franciane
Martins, Daniel F.
Preventive Supplementation of Omega-3 Reduces Pain and Pro-inflammatory Cytokines in a Mouse Model of Complex Regional Pain Syndrome Type I
title Preventive Supplementation of Omega-3 Reduces Pain and Pro-inflammatory Cytokines in a Mouse Model of Complex Regional Pain Syndrome Type I
title_full Preventive Supplementation of Omega-3 Reduces Pain and Pro-inflammatory Cytokines in a Mouse Model of Complex Regional Pain Syndrome Type I
title_fullStr Preventive Supplementation of Omega-3 Reduces Pain and Pro-inflammatory Cytokines in a Mouse Model of Complex Regional Pain Syndrome Type I
title_full_unstemmed Preventive Supplementation of Omega-3 Reduces Pain and Pro-inflammatory Cytokines in a Mouse Model of Complex Regional Pain Syndrome Type I
title_short Preventive Supplementation of Omega-3 Reduces Pain and Pro-inflammatory Cytokines in a Mouse Model of Complex Regional Pain Syndrome Type I
title_sort preventive supplementation of omega-3 reduces pain and pro-inflammatory cytokines in a mouse model of complex regional pain syndrome type i
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9005766/
https://www.ncbi.nlm.nih.gov/pubmed/35431823
http://dx.doi.org/10.3389/fnint.2022.840249
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