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Diagnostic Performance of a Novel CXCL10 mRNA Release Assay for Mycobacterium tuberculosis Infection

One-fourth of the world’s population has been infected with Mycobacterium tuberculosis (M.tb). Although interferon-gamma release assays (IGRAs) have been shown to be valid methods for identifying M.tb infection and auxiliary methods for diagnosis of active tuberculosis (TB), lower sensitivity and hi...

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Autores principales: Pan, Liping, Huang, Mailing, Jia, Hongyan, Deng, Guofang, Chen, Yu, Wei, Rongrong, Zhang, Mingxia, Li, Xin, Sun, Qi, Fang, Mutong, Ren, Pengfei, Xing, Aiying, Chen, Qi, Li, Xinxin, Du, Boping, Chen, Tao, Gao, Mengqiu, Zhang, Zongde
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9005954/
https://www.ncbi.nlm.nih.gov/pubmed/35432271
http://dx.doi.org/10.3389/fmicb.2022.825413
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author Pan, Liping
Huang, Mailing
Jia, Hongyan
Deng, Guofang
Chen, Yu
Wei, Rongrong
Zhang, Mingxia
Li, Xin
Sun, Qi
Fang, Mutong
Ren, Pengfei
Xing, Aiying
Chen, Qi
Li, Xinxin
Du, Boping
Chen, Tao
Gao, Mengqiu
Zhang, Zongde
author_facet Pan, Liping
Huang, Mailing
Jia, Hongyan
Deng, Guofang
Chen, Yu
Wei, Rongrong
Zhang, Mingxia
Li, Xin
Sun, Qi
Fang, Mutong
Ren, Pengfei
Xing, Aiying
Chen, Qi
Li, Xinxin
Du, Boping
Chen, Tao
Gao, Mengqiu
Zhang, Zongde
author_sort Pan, Liping
collection PubMed
description One-fourth of the world’s population has been infected with Mycobacterium tuberculosis (M.tb). Although interferon-gamma release assays (IGRAs) have been shown to be valid methods for identifying M.tb infection and auxiliary methods for diagnosis of active tuberculosis (TB), lower sensitivity and higher indeterminate rate were often detected among immunosuppressed patients. IP-10 was an alternative biomarker due to the higher expression level after M.tb antigen stimulation, but whether CXCL10 mRNA (the gene that transcribes for the IP-10 protein) can be used as a target for M.tb infection diagnosis was limited. Therefore, we aimed to evaluate the performance of a novel M.tb-specific CXCL10 mRNA release assay in diagnosis of M.tb infection. Suspected TB patients and healthy controls were prospectively recruited between March 2018 and November 2019 from three hospitals in China. CXCL10 mRNA release assay and traditional interferon-gamma release assay (T-SPOT.TB) were simultaneously performed on peripheral blood. Of the 1,479 participants enrolled in the study, 352 patients with definite TB and 153 healthy controls were analyzed. CXCL10 mRNA release assay provided a sensitivity of 93.9% (95% CI = 90.8–96.2%) and a specificity of 98.0% (95% CI = 94.3–99.6%) in the diagnosis of M.tb infection, respectively, while T-SPOT.TB gave a sensitivity of 94.5% (95% CI = 91.5–96.6%) and a specificity of 100% (95% CI = 97.6–100.0%) in the diagnosis of M.tb infection, respectively. The diagnostic performance of CXCL10 mRNA release assay was consistent with T-SPOT.TB, with a total coincidence rate of 95.0% (95% CI = 93.0–96.9%) and a Cohen’s kappa value of 0.89 (0.84–0.93, p < 0.001). However, among TB patients with HIV co-infection (n = 14), CXCL10 mRNA release assay presented significantly higher positive rate [92.9% (66.1–99.8%) vs. 61.5% (31.6–86.1%), p = 0.029] than those of T-SPOT.TB. These results suggested that M.tb-specific CXCL10 mRNA was a novel and useful target in the diagnosis of M.tb infection.
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spelling pubmed-90059542022-04-14 Diagnostic Performance of a Novel CXCL10 mRNA Release Assay for Mycobacterium tuberculosis Infection Pan, Liping Huang, Mailing Jia, Hongyan Deng, Guofang Chen, Yu Wei, Rongrong Zhang, Mingxia Li, Xin Sun, Qi Fang, Mutong Ren, Pengfei Xing, Aiying Chen, Qi Li, Xinxin Du, Boping Chen, Tao Gao, Mengqiu Zhang, Zongde Front Microbiol Microbiology One-fourth of the world’s population has been infected with Mycobacterium tuberculosis (M.tb). Although interferon-gamma release assays (IGRAs) have been shown to be valid methods for identifying M.tb infection and auxiliary methods for diagnosis of active tuberculosis (TB), lower sensitivity and higher indeterminate rate were often detected among immunosuppressed patients. IP-10 was an alternative biomarker due to the higher expression level after M.tb antigen stimulation, but whether CXCL10 mRNA (the gene that transcribes for the IP-10 protein) can be used as a target for M.tb infection diagnosis was limited. Therefore, we aimed to evaluate the performance of a novel M.tb-specific CXCL10 mRNA release assay in diagnosis of M.tb infection. Suspected TB patients and healthy controls were prospectively recruited between March 2018 and November 2019 from three hospitals in China. CXCL10 mRNA release assay and traditional interferon-gamma release assay (T-SPOT.TB) were simultaneously performed on peripheral blood. Of the 1,479 participants enrolled in the study, 352 patients with definite TB and 153 healthy controls were analyzed. CXCL10 mRNA release assay provided a sensitivity of 93.9% (95% CI = 90.8–96.2%) and a specificity of 98.0% (95% CI = 94.3–99.6%) in the diagnosis of M.tb infection, respectively, while T-SPOT.TB gave a sensitivity of 94.5% (95% CI = 91.5–96.6%) and a specificity of 100% (95% CI = 97.6–100.0%) in the diagnosis of M.tb infection, respectively. The diagnostic performance of CXCL10 mRNA release assay was consistent with T-SPOT.TB, with a total coincidence rate of 95.0% (95% CI = 93.0–96.9%) and a Cohen’s kappa value of 0.89 (0.84–0.93, p < 0.001). However, among TB patients with HIV co-infection (n = 14), CXCL10 mRNA release assay presented significantly higher positive rate [92.9% (66.1–99.8%) vs. 61.5% (31.6–86.1%), p = 0.029] than those of T-SPOT.TB. These results suggested that M.tb-specific CXCL10 mRNA was a novel and useful target in the diagnosis of M.tb infection. Frontiers Media S.A. 2022-03-30 /pmc/articles/PMC9005954/ /pubmed/35432271 http://dx.doi.org/10.3389/fmicb.2022.825413 Text en Copyright © 2022 Pan, Huang, Jia, Deng, Chen, Wei, Zhang, Li, Sun, Fang, Ren, Xing, Chen, Li, Du, Chen, Gao and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Pan, Liping
Huang, Mailing
Jia, Hongyan
Deng, Guofang
Chen, Yu
Wei, Rongrong
Zhang, Mingxia
Li, Xin
Sun, Qi
Fang, Mutong
Ren, Pengfei
Xing, Aiying
Chen, Qi
Li, Xinxin
Du, Boping
Chen, Tao
Gao, Mengqiu
Zhang, Zongde
Diagnostic Performance of a Novel CXCL10 mRNA Release Assay for Mycobacterium tuberculosis Infection
title Diagnostic Performance of a Novel CXCL10 mRNA Release Assay for Mycobacterium tuberculosis Infection
title_full Diagnostic Performance of a Novel CXCL10 mRNA Release Assay for Mycobacterium tuberculosis Infection
title_fullStr Diagnostic Performance of a Novel CXCL10 mRNA Release Assay for Mycobacterium tuberculosis Infection
title_full_unstemmed Diagnostic Performance of a Novel CXCL10 mRNA Release Assay for Mycobacterium tuberculosis Infection
title_short Diagnostic Performance of a Novel CXCL10 mRNA Release Assay for Mycobacterium tuberculosis Infection
title_sort diagnostic performance of a novel cxcl10 mrna release assay for mycobacterium tuberculosis infection
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9005954/
https://www.ncbi.nlm.nih.gov/pubmed/35432271
http://dx.doi.org/10.3389/fmicb.2022.825413
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