Cargando…
Seamless and early gap healing of osteochondral defects by autologous mosaicplasty combined with bioactive supramolecular nanofiber-enabled gelatin methacryloyl (BSN-GelMA) hydrogel
Autologous mosaicplasty is a common approach used to treat osteochondral defects in clinical practice. Gap integration between host and transplanted plugs requires bone tissue reservation and hyaline cartilage regeneration without uneven surface, graft necrosis and sclerosis. However, poor gap integ...
Autores principales: | , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
KeAi Publishing
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9005961/ https://www.ncbi.nlm.nih.gov/pubmed/35441114 http://dx.doi.org/10.1016/j.bioactmat.2022.03.038 |
_version_ | 1784686572143116288 |
---|---|
author | Wu, Hongwei Shang, Yuna Sun, Wei Ouyang, Xinyi Zhou, Wenyan Lu, Jieji Yang, Shuhui Wei, Wei Yao, Xudong Wang, Xiaozhao Zhang, Xianzhu Chen, Yishan He, Qiulin Yang, Zhimou Ouyang, Hongwei |
author_facet | Wu, Hongwei Shang, Yuna Sun, Wei Ouyang, Xinyi Zhou, Wenyan Lu, Jieji Yang, Shuhui Wei, Wei Yao, Xudong Wang, Xiaozhao Zhang, Xianzhu Chen, Yishan He, Qiulin Yang, Zhimou Ouyang, Hongwei |
author_sort | Wu, Hongwei |
collection | PubMed |
description | Autologous mosaicplasty is a common approach used to treat osteochondral defects in clinical practice. Gap integration between host and transplanted plugs requires bone tissue reservation and hyaline cartilage regeneration without uneven surface, graft necrosis and sclerosis. However, poor gap integration is a serious concern, which eventually leads to deterioration of joint function. To deal with such complications, this study has developed a strategy to effectively enhance integration of the gap region following mosaicplasty by applying injectable bioactive supramolecular nanofiber-enabled gelatin methacryloyl (GelMA) hydrogel (BSN-GelMA). A rabbit osteochondral defect model demonstrated that BSN-GelMA achieved seamless osteochondral healing in the gap region between plugs of osteochondral defects following mosaicplasty, as early as six weeks. Moreover, the International Cartilage Repair Society score, histology score, glycosaminoglycan content, subchondral bone volume, and collagen II expression were observed to be the highest in the gap region of BSN-GelMA treated group. This improved outcome was due to bio-interactive materials, which acted as tissue fillers to bridge the gap, prevent cartilage degeneration, and promote graft survival and migration of bone marrow mesenchymal stem cells by releasing bioactive supramolecular nanofibers from the GelMA hydrogel. This study provides a powerful and applicable approach to improve gap integration after autologous mosaicplasty. It is also a promising off-the-shelf bioactive material for cell-free in situ tissue regeneration. |
format | Online Article Text |
id | pubmed-9005961 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | KeAi Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-90059612022-04-18 Seamless and early gap healing of osteochondral defects by autologous mosaicplasty combined with bioactive supramolecular nanofiber-enabled gelatin methacryloyl (BSN-GelMA) hydrogel Wu, Hongwei Shang, Yuna Sun, Wei Ouyang, Xinyi Zhou, Wenyan Lu, Jieji Yang, Shuhui Wei, Wei Yao, Xudong Wang, Xiaozhao Zhang, Xianzhu Chen, Yishan He, Qiulin Yang, Zhimou Ouyang, Hongwei Bioact Mater Article Autologous mosaicplasty is a common approach used to treat osteochondral defects in clinical practice. Gap integration between host and transplanted plugs requires bone tissue reservation and hyaline cartilage regeneration without uneven surface, graft necrosis and sclerosis. However, poor gap integration is a serious concern, which eventually leads to deterioration of joint function. To deal with such complications, this study has developed a strategy to effectively enhance integration of the gap region following mosaicplasty by applying injectable bioactive supramolecular nanofiber-enabled gelatin methacryloyl (GelMA) hydrogel (BSN-GelMA). A rabbit osteochondral defect model demonstrated that BSN-GelMA achieved seamless osteochondral healing in the gap region between plugs of osteochondral defects following mosaicplasty, as early as six weeks. Moreover, the International Cartilage Repair Society score, histology score, glycosaminoglycan content, subchondral bone volume, and collagen II expression were observed to be the highest in the gap region of BSN-GelMA treated group. This improved outcome was due to bio-interactive materials, which acted as tissue fillers to bridge the gap, prevent cartilage degeneration, and promote graft survival and migration of bone marrow mesenchymal stem cells by releasing bioactive supramolecular nanofibers from the GelMA hydrogel. This study provides a powerful and applicable approach to improve gap integration after autologous mosaicplasty. It is also a promising off-the-shelf bioactive material for cell-free in situ tissue regeneration. KeAi Publishing 2022-04-05 /pmc/articles/PMC9005961/ /pubmed/35441114 http://dx.doi.org/10.1016/j.bioactmat.2022.03.038 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Wu, Hongwei Shang, Yuna Sun, Wei Ouyang, Xinyi Zhou, Wenyan Lu, Jieji Yang, Shuhui Wei, Wei Yao, Xudong Wang, Xiaozhao Zhang, Xianzhu Chen, Yishan He, Qiulin Yang, Zhimou Ouyang, Hongwei Seamless and early gap healing of osteochondral defects by autologous mosaicplasty combined with bioactive supramolecular nanofiber-enabled gelatin methacryloyl (BSN-GelMA) hydrogel |
title | Seamless and early gap healing of osteochondral defects by autologous mosaicplasty combined with bioactive supramolecular nanofiber-enabled gelatin methacryloyl (BSN-GelMA) hydrogel |
title_full | Seamless and early gap healing of osteochondral defects by autologous mosaicplasty combined with bioactive supramolecular nanofiber-enabled gelatin methacryloyl (BSN-GelMA) hydrogel |
title_fullStr | Seamless and early gap healing of osteochondral defects by autologous mosaicplasty combined with bioactive supramolecular nanofiber-enabled gelatin methacryloyl (BSN-GelMA) hydrogel |
title_full_unstemmed | Seamless and early gap healing of osteochondral defects by autologous mosaicplasty combined with bioactive supramolecular nanofiber-enabled gelatin methacryloyl (BSN-GelMA) hydrogel |
title_short | Seamless and early gap healing of osteochondral defects by autologous mosaicplasty combined with bioactive supramolecular nanofiber-enabled gelatin methacryloyl (BSN-GelMA) hydrogel |
title_sort | seamless and early gap healing of osteochondral defects by autologous mosaicplasty combined with bioactive supramolecular nanofiber-enabled gelatin methacryloyl (bsn-gelma) hydrogel |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9005961/ https://www.ncbi.nlm.nih.gov/pubmed/35441114 http://dx.doi.org/10.1016/j.bioactmat.2022.03.038 |
work_keys_str_mv | AT wuhongwei seamlessandearlygaphealingofosteochondraldefectsbyautologousmosaicplastycombinedwithbioactivesupramolecularnanofiberenabledgelatinmethacryloylbsngelmahydrogel AT shangyuna seamlessandearlygaphealingofosteochondraldefectsbyautologousmosaicplastycombinedwithbioactivesupramolecularnanofiberenabledgelatinmethacryloylbsngelmahydrogel AT sunwei seamlessandearlygaphealingofosteochondraldefectsbyautologousmosaicplastycombinedwithbioactivesupramolecularnanofiberenabledgelatinmethacryloylbsngelmahydrogel AT ouyangxinyi seamlessandearlygaphealingofosteochondraldefectsbyautologousmosaicplastycombinedwithbioactivesupramolecularnanofiberenabledgelatinmethacryloylbsngelmahydrogel AT zhouwenyan seamlessandearlygaphealingofosteochondraldefectsbyautologousmosaicplastycombinedwithbioactivesupramolecularnanofiberenabledgelatinmethacryloylbsngelmahydrogel AT lujieji seamlessandearlygaphealingofosteochondraldefectsbyautologousmosaicplastycombinedwithbioactivesupramolecularnanofiberenabledgelatinmethacryloylbsngelmahydrogel AT yangshuhui seamlessandearlygaphealingofosteochondraldefectsbyautologousmosaicplastycombinedwithbioactivesupramolecularnanofiberenabledgelatinmethacryloylbsngelmahydrogel AT weiwei seamlessandearlygaphealingofosteochondraldefectsbyautologousmosaicplastycombinedwithbioactivesupramolecularnanofiberenabledgelatinmethacryloylbsngelmahydrogel AT yaoxudong seamlessandearlygaphealingofosteochondraldefectsbyautologousmosaicplastycombinedwithbioactivesupramolecularnanofiberenabledgelatinmethacryloylbsngelmahydrogel AT wangxiaozhao seamlessandearlygaphealingofosteochondraldefectsbyautologousmosaicplastycombinedwithbioactivesupramolecularnanofiberenabledgelatinmethacryloylbsngelmahydrogel AT zhangxianzhu seamlessandearlygaphealingofosteochondraldefectsbyautologousmosaicplastycombinedwithbioactivesupramolecularnanofiberenabledgelatinmethacryloylbsngelmahydrogel AT chenyishan seamlessandearlygaphealingofosteochondraldefectsbyautologousmosaicplastycombinedwithbioactivesupramolecularnanofiberenabledgelatinmethacryloylbsngelmahydrogel AT heqiulin seamlessandearlygaphealingofosteochondraldefectsbyautologousmosaicplastycombinedwithbioactivesupramolecularnanofiberenabledgelatinmethacryloylbsngelmahydrogel AT yangzhimou seamlessandearlygaphealingofosteochondraldefectsbyautologousmosaicplastycombinedwithbioactivesupramolecularnanofiberenabledgelatinmethacryloylbsngelmahydrogel AT ouyanghongwei seamlessandearlygaphealingofosteochondraldefectsbyautologousmosaicplastycombinedwithbioactivesupramolecularnanofiberenabledgelatinmethacryloylbsngelmahydrogel |