Evaluation of rapid antigen detection kits for detection of SARS-CoV-2 B.1.617.2 virus

Aim: Currently, there is lack of data regarding rapid antigen detection (RAD) kits to detect SARS-CoV-2 B.1.617.2 virus. Objective: The purpose of this evaluation is to assess analytical sensitivity of 12 RAD kits against SARS-CoV-2 B.1.617.2. Study design: Analytical sensitivity was determined by l...

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Autores principales: Mak, Gannon CK, Lau, Stephen SY, Wong, Kitty KY, Chow, Nancy LS, Lau, Chi-Shan, Lam, Edman TK, Ng, Ken HL, Chan, Rickjason CW
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Future Medicine Ltd 2022
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9006337/
https://www.ncbi.nlm.nih.gov/pubmed/35432576
http://dx.doi.org/10.2217/fvl-2021-0229
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author Mak, Gannon CK
Lau, Stephen SY
Wong, Kitty KY
Chow, Nancy LS
Lau, Chi-Shan
Lam, Edman TK
Ng, Ken HL
Chan, Rickjason CW
author_facet Mak, Gannon CK
Lau, Stephen SY
Wong, Kitty KY
Chow, Nancy LS
Lau, Chi-Shan
Lam, Edman TK
Ng, Ken HL
Chan, Rickjason CW
author_sort Mak, Gannon CK
collection PubMed
description Aim: Currently, there is lack of data regarding rapid antigen detection (RAD) kits to detect SARS-CoV-2 B.1.617.2 virus. Objective: The purpose of this evaluation is to assess analytical sensitivity of 12 RAD kits against SARS-CoV-2 B.1.617.2. Study design: Analytical sensitivity was determined by limit of detection (LOD). A serial tenfold dilution set from a respiratory specimen collected from a COVID-19 patient infected by SARS-CoV-2 B.1.617.2 was used. RT-PCR was used as a reference method. Results: The LOD results showed that 11 and one RAD kits were 100- and 1000-fold less sensitive than RT-PCR respectively. Conclusion: The results showed that the RAD kits evaluated in this study may be used for first-line screening of the SARS-CoV-2 B.1.617.2 variant.
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spelling pubmed-90063372022-04-15 Evaluation of rapid antigen detection kits for detection of SARS-CoV-2 B.1.617.2 virus Mak, Gannon CK Lau, Stephen SY Wong, Kitty KY Chow, Nancy LS Lau, Chi-Shan Lam, Edman TK Ng, Ken HL Chan, Rickjason CW Future Virol Short Communication Aim: Currently, there is lack of data regarding rapid antigen detection (RAD) kits to detect SARS-CoV-2 B.1.617.2 virus. Objective: The purpose of this evaluation is to assess analytical sensitivity of 12 RAD kits against SARS-CoV-2 B.1.617.2. Study design: Analytical sensitivity was determined by limit of detection (LOD). A serial tenfold dilution set from a respiratory specimen collected from a COVID-19 patient infected by SARS-CoV-2 B.1.617.2 was used. RT-PCR was used as a reference method. Results: The LOD results showed that 11 and one RAD kits were 100- and 1000-fold less sensitive than RT-PCR respectively. Conclusion: The results showed that the RAD kits evaluated in this study may be used for first-line screening of the SARS-CoV-2 B.1.617.2 variant. Future Medicine Ltd 2022-04-11 2022-03 /pmc/articles/PMC9006337/ /pubmed/35432576 http://dx.doi.org/10.2217/fvl-2021-0229 Text en © 2022 Future Medicine Ltd https://creativecommons.org/licenses/by/4.0/This work is licensed under the Creative Commons Attribution 4.0 License (https://creativecommons.org/licenses/by/4.0/)
spellingShingle Short Communication
Mak, Gannon CK
Lau, Stephen SY
Wong, Kitty KY
Chow, Nancy LS
Lau, Chi-Shan
Lam, Edman TK
Ng, Ken HL
Chan, Rickjason CW
Evaluation of rapid antigen detection kits for detection of SARS-CoV-2 B.1.617.2 virus
title Evaluation of rapid antigen detection kits for detection of SARS-CoV-2 B.1.617.2 virus
title_full Evaluation of rapid antigen detection kits for detection of SARS-CoV-2 B.1.617.2 virus
title_fullStr Evaluation of rapid antigen detection kits for detection of SARS-CoV-2 B.1.617.2 virus
title_full_unstemmed Evaluation of rapid antigen detection kits for detection of SARS-CoV-2 B.1.617.2 virus
title_short Evaluation of rapid antigen detection kits for detection of SARS-CoV-2 B.1.617.2 virus
title_sort evaluation of rapid antigen detection kits for detection of sars-cov-2 b.1.617.2 virus
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9006337/
https://www.ncbi.nlm.nih.gov/pubmed/35432576
http://dx.doi.org/10.2217/fvl-2021-0229
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