Cargando…
Complete Chloroplast Genomes and Comparative Analyses of Three Ornamental Impatiens Species
Impatiens L., the largest genus in the family Balsaminaceae with approximately 1,000 species, is a controversial genus. Due to the conflict of morphological features and insufficient genomic resources, the studies of systematic evolution and understanding of taxonomic identification are considered t...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9006450/ https://www.ncbi.nlm.nih.gov/pubmed/35432470 http://dx.doi.org/10.3389/fgene.2022.816123 |
_version_ | 1784686667860279296 |
---|---|
author | Luo, Chao Huang, Wulue Yer, Huseyin Kamuda, Troy Li, Xinyi Li, Yang Rong, Yuhong Yan, Bo Wen, Yonghui Wang, Qiong Huang, Meijuan Huang, Haiquan |
author_facet | Luo, Chao Huang, Wulue Yer, Huseyin Kamuda, Troy Li, Xinyi Li, Yang Rong, Yuhong Yan, Bo Wen, Yonghui Wang, Qiong Huang, Meijuan Huang, Haiquan |
author_sort | Luo, Chao |
collection | PubMed |
description | Impatiens L., the largest genus in the family Balsaminaceae with approximately 1,000 species, is a controversial genus. Due to the conflict of morphological features and insufficient genomic resources, the studies of systematic evolution and understanding of taxonomic identification are considered to be very limited. Hence, we have sequenced the complete chloroplast genomes of three ornamental species (Impatiens balsamina, I. hawkeri, and I. walleriana), and compared them with previously published wild species data. We performed a detailed comparison of a highly similar basic structure, size, GC content, gene number, order, and functional array among them. Similarly, most divergent genes were detected from previous work in the literature. The mutational regions containing highly variable nucleotide hotspots were identified and may be used as potential markers for species identification and taxonomy. Furthermore, using whole chloroplast genome data to analysis the phylogenetic relationship of the Balsaminaceae species, we found that they were all part of a single clade. The three phenotypically different ornamental species were clustered together, suggesting that they were very likely to be closely related. We achieved and characterized the plastid genome structure, identified the divergence hotspots, and determined the phylogenetic and taxonomic positions of the three cultivated species in the Impatiens genus. The results may show that the chloroplast genome can be used to solve phylogenetic problems in or between the Impatiens genus and also provide genomic resources for the study of the Balsaminaceae family’s systematics and evolution. |
format | Online Article Text |
id | pubmed-9006450 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-90064502022-04-14 Complete Chloroplast Genomes and Comparative Analyses of Three Ornamental Impatiens Species Luo, Chao Huang, Wulue Yer, Huseyin Kamuda, Troy Li, Xinyi Li, Yang Rong, Yuhong Yan, Bo Wen, Yonghui Wang, Qiong Huang, Meijuan Huang, Haiquan Front Genet Genetics Impatiens L., the largest genus in the family Balsaminaceae with approximately 1,000 species, is a controversial genus. Due to the conflict of morphological features and insufficient genomic resources, the studies of systematic evolution and understanding of taxonomic identification are considered to be very limited. Hence, we have sequenced the complete chloroplast genomes of three ornamental species (Impatiens balsamina, I. hawkeri, and I. walleriana), and compared them with previously published wild species data. We performed a detailed comparison of a highly similar basic structure, size, GC content, gene number, order, and functional array among them. Similarly, most divergent genes were detected from previous work in the literature. The mutational regions containing highly variable nucleotide hotspots were identified and may be used as potential markers for species identification and taxonomy. Furthermore, using whole chloroplast genome data to analysis the phylogenetic relationship of the Balsaminaceae species, we found that they were all part of a single clade. The three phenotypically different ornamental species were clustered together, suggesting that they were very likely to be closely related. We achieved and characterized the plastid genome structure, identified the divergence hotspots, and determined the phylogenetic and taxonomic positions of the three cultivated species in the Impatiens genus. The results may show that the chloroplast genome can be used to solve phylogenetic problems in or between the Impatiens genus and also provide genomic resources for the study of the Balsaminaceae family’s systematics and evolution. Frontiers Media S.A. 2022-03-30 /pmc/articles/PMC9006450/ /pubmed/35432470 http://dx.doi.org/10.3389/fgene.2022.816123 Text en Copyright © 2022 Luo, Huang, Yer, Kamuda, Li, Li, Rong, Yan, Wen, Wang, Huang and Huang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Luo, Chao Huang, Wulue Yer, Huseyin Kamuda, Troy Li, Xinyi Li, Yang Rong, Yuhong Yan, Bo Wen, Yonghui Wang, Qiong Huang, Meijuan Huang, Haiquan Complete Chloroplast Genomes and Comparative Analyses of Three Ornamental Impatiens Species |
title | Complete Chloroplast Genomes and Comparative Analyses of Three Ornamental Impatiens Species |
title_full | Complete Chloroplast Genomes and Comparative Analyses of Three Ornamental Impatiens Species |
title_fullStr | Complete Chloroplast Genomes and Comparative Analyses of Three Ornamental Impatiens Species |
title_full_unstemmed | Complete Chloroplast Genomes and Comparative Analyses of Three Ornamental Impatiens Species |
title_short | Complete Chloroplast Genomes and Comparative Analyses of Three Ornamental Impatiens Species |
title_sort | complete chloroplast genomes and comparative analyses of three ornamental impatiens species |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9006450/ https://www.ncbi.nlm.nih.gov/pubmed/35432470 http://dx.doi.org/10.3389/fgene.2022.816123 |
work_keys_str_mv | AT luochao completechloroplastgenomesandcomparativeanalysesofthreeornamentalimpatiensspecies AT huangwulue completechloroplastgenomesandcomparativeanalysesofthreeornamentalimpatiensspecies AT yerhuseyin completechloroplastgenomesandcomparativeanalysesofthreeornamentalimpatiensspecies AT kamudatroy completechloroplastgenomesandcomparativeanalysesofthreeornamentalimpatiensspecies AT lixinyi completechloroplastgenomesandcomparativeanalysesofthreeornamentalimpatiensspecies AT liyang completechloroplastgenomesandcomparativeanalysesofthreeornamentalimpatiensspecies AT rongyuhong completechloroplastgenomesandcomparativeanalysesofthreeornamentalimpatiensspecies AT yanbo completechloroplastgenomesandcomparativeanalysesofthreeornamentalimpatiensspecies AT wenyonghui completechloroplastgenomesandcomparativeanalysesofthreeornamentalimpatiensspecies AT wangqiong completechloroplastgenomesandcomparativeanalysesofthreeornamentalimpatiensspecies AT huangmeijuan completechloroplastgenomesandcomparativeanalysesofthreeornamentalimpatiensspecies AT huanghaiquan completechloroplastgenomesandcomparativeanalysesofthreeornamentalimpatiensspecies |