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Complete Chloroplast Genomes and Comparative Analyses of Three Ornamental Impatiens Species

Impatiens L., the largest genus in the family Balsaminaceae with approximately 1,000 species, is a controversial genus. Due to the conflict of morphological features and insufficient genomic resources, the studies of systematic evolution and understanding of taxonomic identification are considered t...

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Autores principales: Luo, Chao, Huang, Wulue, Yer, Huseyin, Kamuda, Troy, Li, Xinyi, Li, Yang, Rong, Yuhong, Yan, Bo, Wen, Yonghui, Wang, Qiong, Huang, Meijuan, Huang, Haiquan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9006450/
https://www.ncbi.nlm.nih.gov/pubmed/35432470
http://dx.doi.org/10.3389/fgene.2022.816123
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author Luo, Chao
Huang, Wulue
Yer, Huseyin
Kamuda, Troy
Li, Xinyi
Li, Yang
Rong, Yuhong
Yan, Bo
Wen, Yonghui
Wang, Qiong
Huang, Meijuan
Huang, Haiquan
author_facet Luo, Chao
Huang, Wulue
Yer, Huseyin
Kamuda, Troy
Li, Xinyi
Li, Yang
Rong, Yuhong
Yan, Bo
Wen, Yonghui
Wang, Qiong
Huang, Meijuan
Huang, Haiquan
author_sort Luo, Chao
collection PubMed
description Impatiens L., the largest genus in the family Balsaminaceae with approximately 1,000 species, is a controversial genus. Due to the conflict of morphological features and insufficient genomic resources, the studies of systematic evolution and understanding of taxonomic identification are considered to be very limited. Hence, we have sequenced the complete chloroplast genomes of three ornamental species (Impatiens balsamina, I. hawkeri, and I. walleriana), and compared them with previously published wild species data. We performed a detailed comparison of a highly similar basic structure, size, GC content, gene number, order, and functional array among them. Similarly, most divergent genes were detected from previous work in the literature. The mutational regions containing highly variable nucleotide hotspots were identified and may be used as potential markers for species identification and taxonomy. Furthermore, using whole chloroplast genome data to analysis the phylogenetic relationship of the Balsaminaceae species, we found that they were all part of a single clade. The three phenotypically different ornamental species were clustered together, suggesting that they were very likely to be closely related. We achieved and characterized the plastid genome structure, identified the divergence hotspots, and determined the phylogenetic and taxonomic positions of the three cultivated species in the Impatiens genus. The results may show that the chloroplast genome can be used to solve phylogenetic problems in or between the Impatiens genus and also provide genomic resources for the study of the Balsaminaceae family’s systematics and evolution.
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spelling pubmed-90064502022-04-14 Complete Chloroplast Genomes and Comparative Analyses of Three Ornamental Impatiens Species Luo, Chao Huang, Wulue Yer, Huseyin Kamuda, Troy Li, Xinyi Li, Yang Rong, Yuhong Yan, Bo Wen, Yonghui Wang, Qiong Huang, Meijuan Huang, Haiquan Front Genet Genetics Impatiens L., the largest genus in the family Balsaminaceae with approximately 1,000 species, is a controversial genus. Due to the conflict of morphological features and insufficient genomic resources, the studies of systematic evolution and understanding of taxonomic identification are considered to be very limited. Hence, we have sequenced the complete chloroplast genomes of three ornamental species (Impatiens balsamina, I. hawkeri, and I. walleriana), and compared them with previously published wild species data. We performed a detailed comparison of a highly similar basic structure, size, GC content, gene number, order, and functional array among them. Similarly, most divergent genes were detected from previous work in the literature. The mutational regions containing highly variable nucleotide hotspots were identified and may be used as potential markers for species identification and taxonomy. Furthermore, using whole chloroplast genome data to analysis the phylogenetic relationship of the Balsaminaceae species, we found that they were all part of a single clade. The three phenotypically different ornamental species were clustered together, suggesting that they were very likely to be closely related. We achieved and characterized the plastid genome structure, identified the divergence hotspots, and determined the phylogenetic and taxonomic positions of the three cultivated species in the Impatiens genus. The results may show that the chloroplast genome can be used to solve phylogenetic problems in or between the Impatiens genus and also provide genomic resources for the study of the Balsaminaceae family’s systematics and evolution. Frontiers Media S.A. 2022-03-30 /pmc/articles/PMC9006450/ /pubmed/35432470 http://dx.doi.org/10.3389/fgene.2022.816123 Text en Copyright © 2022 Luo, Huang, Yer, Kamuda, Li, Li, Rong, Yan, Wen, Wang, Huang and Huang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Luo, Chao
Huang, Wulue
Yer, Huseyin
Kamuda, Troy
Li, Xinyi
Li, Yang
Rong, Yuhong
Yan, Bo
Wen, Yonghui
Wang, Qiong
Huang, Meijuan
Huang, Haiquan
Complete Chloroplast Genomes and Comparative Analyses of Three Ornamental Impatiens Species
title Complete Chloroplast Genomes and Comparative Analyses of Three Ornamental Impatiens Species
title_full Complete Chloroplast Genomes and Comparative Analyses of Three Ornamental Impatiens Species
title_fullStr Complete Chloroplast Genomes and Comparative Analyses of Three Ornamental Impatiens Species
title_full_unstemmed Complete Chloroplast Genomes and Comparative Analyses of Three Ornamental Impatiens Species
title_short Complete Chloroplast Genomes and Comparative Analyses of Three Ornamental Impatiens Species
title_sort complete chloroplast genomes and comparative analyses of three ornamental impatiens species
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9006450/
https://www.ncbi.nlm.nih.gov/pubmed/35432470
http://dx.doi.org/10.3389/fgene.2022.816123
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