A protocol for identifying the binding sites of small molecules on the cystic fibrosis transmembrane conductance regulator (CFTR) protein

We describe a protocol to identify the binding site(s) for a drug called ivacaftor that potentiates the CFTR chloride channel. We use photoaffinity probes—based on the structure of ivacaftor—to covalently modify the CFTR protein at the region that constitutes the drug binding site(s). We define the...

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Detalles Bibliográficos
Autores principales: Laselva, Onofrio, Petrotchenko, Evgeniy V., Hamilton, C. Michael, Qureshi, Zafar, Borchers, Christoph H., Young, Robert N., Bear, Christine E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9006651/
https://www.ncbi.nlm.nih.gov/pubmed/35434660
http://dx.doi.org/10.1016/j.xpro.2022.101258

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9006651/
https://www.ncbi.nlm.nih.gov/pubmed/35434660
http://dx.doi.org/10.1016/j.xpro.2022.101258

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