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Isolation and molecular characterization of clinical and environmental dematiaceous fungi and relatives from Iran

BACKGROUND AND PURPOSE: The frequency and genetic diversity of black fungi in environmental and clinical settings have not been fully studied in Iran. This study aimed to identify and evaluate intra- and inter-species DNA sequence variation and also understand the phylogenetic relationships of melan...

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Autores principales: Shokoohi, Gholamreza, Badali, Hamid, Ahmadi, Bahram, Satoh, Kazuo, Nouripour-Sisakht, Sadegh, Nikaeen, Mahnaz, Gramishoar, Mohsen, Jalalizand, Nilufar, Kianipour, Sahar, Mirhendi, Hossein, Makimura, Koichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Iranian Society of Medical Mycology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9006730/
https://www.ncbi.nlm.nih.gov/pubmed/35528626
http://dx.doi.org/10.18502/cmm.7.3.7798
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author Shokoohi, Gholamreza
Badali, Hamid
Ahmadi, Bahram
Satoh, Kazuo
Nouripour-Sisakht, Sadegh
Nikaeen, Mahnaz
Gramishoar, Mohsen
Jalalizand, Nilufar
Kianipour, Sahar
Mirhendi, Hossein
Makimura, Koichi
author_facet Shokoohi, Gholamreza
Badali, Hamid
Ahmadi, Bahram
Satoh, Kazuo
Nouripour-Sisakht, Sadegh
Nikaeen, Mahnaz
Gramishoar, Mohsen
Jalalizand, Nilufar
Kianipour, Sahar
Mirhendi, Hossein
Makimura, Koichi
author_sort Shokoohi, Gholamreza
collection PubMed
description BACKGROUND AND PURPOSE: The frequency and genetic diversity of black fungi in environmental and clinical settings have not been fully studied in Iran. This study aimed to identify and evaluate intra- and inter-species DNA sequence variation and also understand the phylogenetic relationships of melanized fungi and relatives isolated from different geographical regions of Iran. MATERIALS AND METHODS: In total, 111 clinical and environmental strains of dematiaceous fungi were isolated, and their internal transcribed spacer ribosomal DNA (rDNA) regions were sequenced and analyzed. RESULTS: An inter-species nucleotide sequence diversity rate of 1 to 464 nucleotides was observed between the species. Intra-species differences were found in the strains of Alternaria alternata, Cladosporium cladosporioides, Alternaria tenuissima, Curvularia spicifera, Aureobasidium pullulans, Curvularia hawaiiensis, Neoscytalidium dimidiatum, Alternaria terricola, Alternaria chlamydospora, Didymella glomerata, and Drechslera dematioidea by 0–59, 0–22, 0–4, 0–4, 0–3, 0–2, 0–2, 0–2, 0–2, 0–1, and 0–1 nucleotide, respectively. CONCLUSION: The internal transcribed spacer rDNA is useful for the discrimination of several taxa of dematiaceous fungi. However, a better understanding of the taxonomy of species of Alternaria requires a larger rDNA region or a library of other gene sequences.
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spelling pubmed-90067302022-05-06 Isolation and molecular characterization of clinical and environmental dematiaceous fungi and relatives from Iran Shokoohi, Gholamreza Badali, Hamid Ahmadi, Bahram Satoh, Kazuo Nouripour-Sisakht, Sadegh Nikaeen, Mahnaz Gramishoar, Mohsen Jalalizand, Nilufar Kianipour, Sahar Mirhendi, Hossein Makimura, Koichi Curr Med Mycol Original Article BACKGROUND AND PURPOSE: The frequency and genetic diversity of black fungi in environmental and clinical settings have not been fully studied in Iran. This study aimed to identify and evaluate intra- and inter-species DNA sequence variation and also understand the phylogenetic relationships of melanized fungi and relatives isolated from different geographical regions of Iran. MATERIALS AND METHODS: In total, 111 clinical and environmental strains of dematiaceous fungi were isolated, and their internal transcribed spacer ribosomal DNA (rDNA) regions were sequenced and analyzed. RESULTS: An inter-species nucleotide sequence diversity rate of 1 to 464 nucleotides was observed between the species. Intra-species differences were found in the strains of Alternaria alternata, Cladosporium cladosporioides, Alternaria tenuissima, Curvularia spicifera, Aureobasidium pullulans, Curvularia hawaiiensis, Neoscytalidium dimidiatum, Alternaria terricola, Alternaria chlamydospora, Didymella glomerata, and Drechslera dematioidea by 0–59, 0–22, 0–4, 0–4, 0–3, 0–2, 0–2, 0–2, 0–2, 0–1, and 0–1 nucleotide, respectively. CONCLUSION: The internal transcribed spacer rDNA is useful for the discrimination of several taxa of dematiaceous fungi. However, a better understanding of the taxonomy of species of Alternaria requires a larger rDNA region or a library of other gene sequences. Iranian Society of Medical Mycology 2021-09 /pmc/articles/PMC9006730/ /pubmed/35528626 http://dx.doi.org/10.18502/cmm.7.3.7798 Text en Copyright: © Current Medical Mycology https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY) License (https://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution and reproduction in any medium, provided appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Shokoohi, Gholamreza
Badali, Hamid
Ahmadi, Bahram
Satoh, Kazuo
Nouripour-Sisakht, Sadegh
Nikaeen, Mahnaz
Gramishoar, Mohsen
Jalalizand, Nilufar
Kianipour, Sahar
Mirhendi, Hossein
Makimura, Koichi
Isolation and molecular characterization of clinical and environmental dematiaceous fungi and relatives from Iran
title Isolation and molecular characterization of clinical and environmental dematiaceous fungi and relatives from Iran
title_full Isolation and molecular characterization of clinical and environmental dematiaceous fungi and relatives from Iran
title_fullStr Isolation and molecular characterization of clinical and environmental dematiaceous fungi and relatives from Iran
title_full_unstemmed Isolation and molecular characterization of clinical and environmental dematiaceous fungi and relatives from Iran
title_short Isolation and molecular characterization of clinical and environmental dematiaceous fungi and relatives from Iran
title_sort isolation and molecular characterization of clinical and environmental dematiaceous fungi and relatives from iran
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9006730/
https://www.ncbi.nlm.nih.gov/pubmed/35528626
http://dx.doi.org/10.18502/cmm.7.3.7798
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