Cargando…

Investigating the anti-inflammatory and analgesic properties of leaves ethanolic extracts of Cedruslibani and Pinusbrutia

This investigation aimed to assess the anti-inflammatory and analgesic effects of Cedruslibani and Pinusbrutia leaves. The anti-inflammatory property was evaluated by Human Red Blood Cells (HRBC) membrane stabilization assay and Albumin denaturation assay using Sodium diclofenac as a positive contro...

Descripción completa

Detalles Bibliográficos
Autores principales: Karrat, Lina, Abajy, Mohammad Yaser, Nayal, Ream
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9006851/
https://www.ncbi.nlm.nih.gov/pubmed/35434396
http://dx.doi.org/10.1016/j.heliyon.2022.e09254
_version_ 1784686747166179328
author Karrat, Lina
Abajy, Mohammad Yaser
Nayal, Ream
author_facet Karrat, Lina
Abajy, Mohammad Yaser
Nayal, Ream
author_sort Karrat, Lina
collection PubMed
description This investigation aimed to assess the anti-inflammatory and analgesic effects of Cedruslibani and Pinusbrutia leaves. The anti-inflammatory property was evaluated by Human Red Blood Cells (HRBC) membrane stabilization assay and Albumin denaturation assay using Sodium diclofenac as a positive control. To evaluate the analgesic property, formalin and tail flick tests were carried out using ethanolic extracts at a dose of 30 mg/kg and gel containing 2% (w/v) of ethanolic extract of each plant. Diclofenac sodium, diclofenac gel 1% and lidocaine gel 2 % were used as positive controls. RESULTS: The effect of inhibiting hemolysis was observed at concentrations (2.5–12.5) μg/ml for P. brutia, and (2.5–25) μg/ml for C. libani. Moreover, albumin denaturation test showed protection effect for both plant extracts with IC(50) of 47.74 and 81.50 μg/ml for C. libani and P. brutia extract, consecutively. In Formalin test, both extracts could significantly reduce paw licking time, and in tail flick test, each plant extract gel showed greater efficacy than diclofenac gel by calculating the maximum possible effect (MPE %) for both extracts and Diclofenac. CONCLUSION: We concluded that both extracts showed in vitro anti-inflammatory activity at different concentrations when compared to standard drug of diclofenac as well as analgesic activity in formalin and tail flick tests.
format Online
Article
Text
id pubmed-9006851
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-90068512022-04-14 Investigating the anti-inflammatory and analgesic properties of leaves ethanolic extracts of Cedruslibani and Pinusbrutia Karrat, Lina Abajy, Mohammad Yaser Nayal, Ream Heliyon Research Article This investigation aimed to assess the anti-inflammatory and analgesic effects of Cedruslibani and Pinusbrutia leaves. The anti-inflammatory property was evaluated by Human Red Blood Cells (HRBC) membrane stabilization assay and Albumin denaturation assay using Sodium diclofenac as a positive control. To evaluate the analgesic property, formalin and tail flick tests were carried out using ethanolic extracts at a dose of 30 mg/kg and gel containing 2% (w/v) of ethanolic extract of each plant. Diclofenac sodium, diclofenac gel 1% and lidocaine gel 2 % were used as positive controls. RESULTS: The effect of inhibiting hemolysis was observed at concentrations (2.5–12.5) μg/ml for P. brutia, and (2.5–25) μg/ml for C. libani. Moreover, albumin denaturation test showed protection effect for both plant extracts with IC(50) of 47.74 and 81.50 μg/ml for C. libani and P. brutia extract, consecutively. In Formalin test, both extracts could significantly reduce paw licking time, and in tail flick test, each plant extract gel showed greater efficacy than diclofenac gel by calculating the maximum possible effect (MPE %) for both extracts and Diclofenac. CONCLUSION: We concluded that both extracts showed in vitro anti-inflammatory activity at different concentrations when compared to standard drug of diclofenac as well as analgesic activity in formalin and tail flick tests. Elsevier 2022-04-05 /pmc/articles/PMC9006851/ /pubmed/35434396 http://dx.doi.org/10.1016/j.heliyon.2022.e09254 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Karrat, Lina
Abajy, Mohammad Yaser
Nayal, Ream
Investigating the anti-inflammatory and analgesic properties of leaves ethanolic extracts of Cedruslibani and Pinusbrutia
title Investigating the anti-inflammatory and analgesic properties of leaves ethanolic extracts of Cedruslibani and Pinusbrutia
title_full Investigating the anti-inflammatory and analgesic properties of leaves ethanolic extracts of Cedruslibani and Pinusbrutia
title_fullStr Investigating the anti-inflammatory and analgesic properties of leaves ethanolic extracts of Cedruslibani and Pinusbrutia
title_full_unstemmed Investigating the anti-inflammatory and analgesic properties of leaves ethanolic extracts of Cedruslibani and Pinusbrutia
title_short Investigating the anti-inflammatory and analgesic properties of leaves ethanolic extracts of Cedruslibani and Pinusbrutia
title_sort investigating the anti-inflammatory and analgesic properties of leaves ethanolic extracts of cedruslibani and pinusbrutia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9006851/
https://www.ncbi.nlm.nih.gov/pubmed/35434396
http://dx.doi.org/10.1016/j.heliyon.2022.e09254
work_keys_str_mv AT karratlina investigatingtheantiinflammatoryandanalgesicpropertiesofleavesethanolicextractsofcedruslibaniandpinusbrutia
AT abajymohammadyaser investigatingtheantiinflammatoryandanalgesicpropertiesofleavesethanolicextractsofcedruslibaniandpinusbrutia
AT nayalream investigatingtheantiinflammatoryandanalgesicpropertiesofleavesethanolicextractsofcedruslibaniandpinusbrutia