Replication and single-cycle delivery of SARS-CoV-2 replicons

Molecular virology tools are critical for basic studies of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and for developing new therapeutics. Experimental systems that do not rely on viruses capable of spread are needed for potential use in lower-containment settings. In this work...

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Detalles Bibliográficos
Autores principales: Ricardo-Lax, Inna, Luna, Joseph M., Thao, Tran Thi Nhu, Le Pen, Jérémie, Yu, Yingpu, Hoffmann, H.-Heinrich, Schneider, William M., Razooky, Brandon S., Fernandez-Martinez, Javier, Schmidt, Fabian, Weisblum, Yiska, Trüeb, Bettina Salome, Berenguer Veiga, Inês, Schmied, Kimberly, Ebert, Nadine, Michailidis, Eleftherios, Peace, Avery, Sánchez-Rivera, Francisco J., Lowe, Scott W., Rout, Michael P., Hatziioannou, Theodora, Bieniasz, Paul D., Poirier, John T., MacDonald, Margaret R., Thiel, Volker, Rice, Charles M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2021
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9007107/
https://www.ncbi.nlm.nih.gov/pubmed/34648371
http://dx.doi.org/10.1126/science.abj8430
Descripción
Sumario:Molecular virology tools are critical for basic studies of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and for developing new therapeutics. Experimental systems that do not rely on viruses capable of spread are needed for potential use in lower-containment settings. In this work, we use a yeast-based reverse genetics system to develop spike-deleted SARS-CoV-2 self-replicating RNAs. These noninfectious self-replicating RNAs, or replicons, can be trans-complemented with viral glycoproteins to generate replicon delivery particles for single-cycle delivery into a range of cell types. This SARS-CoV-2 replicon system represents a convenient and versatile platform for antiviral drug screening, neutralization assays, host factor validation, and viral variant characterization.

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