Immunomodulatory effects of the polysaccharide from Sinonovacula constricta on RAW264.7 macrophage cells

This study aimed to evaluate the immunomodulatory effect of the polysaccharide from Sinonovacula constricta (SCP‐1‐1) in RAW264.7 cells. SCP‐1‐1 with a molecular weight of 440.0 kDa consisted of glucose and mannose. The immunomodulatory assay results showed that SCP‐1‐1 could significantly enhance p...

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Detalles Bibliográficos
Autores principales: Liu, Zhidong, Liu, Zhifang, Li, Laihao, Zhang, Junjie, Zhao, Qiancheng, Lin, Na, Zhong, Wenzhu, Jiang, Mei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Original Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9007286/
https://www.ncbi.nlm.nih.gov/pubmed/35432971
http://dx.doi.org/10.1002/fsn3.2735
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author Liu, Zhidong
Liu, Zhifang
Li, Laihao
Zhang, Junjie
Zhao, Qiancheng
Lin, Na
Zhong, Wenzhu
Jiang, Mei
author_facet Liu, Zhidong
Liu, Zhifang
Li, Laihao
Zhang, Junjie
Zhao, Qiancheng
Lin, Na
Zhong, Wenzhu
Jiang, Mei
author_sort Liu, Zhidong
collection PubMed
description This study aimed to evaluate the immunomodulatory effect of the polysaccharide from Sinonovacula constricta (SCP‐1‐1) in RAW264.7 cells. SCP‐1‐1 with a molecular weight of 440.0 kDa consisted of glucose and mannose. The immunomodulatory assay results showed that SCP‐1‐1 could significantly enhance phagocytic ability, NO production, and some cytokines (TNF‐α, IL‐6, and IL‐1β) secretion of RAW264.7 cell in a dose‐dependent manner. Western blot analysis results demonstrated that SCP‐1‐1 could regulate the expression levels of the key proteins in the signaling pathways of RAW264.7 cell and might associated with NF‐κβ and PI3K signaling pathway. These findings could contribute to elucidate the immunomodulatory activities of the polysaccharide from Sinonovacula constricta.
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spelling pubmed-90072862022-04-15 Immunomodulatory effects of the polysaccharide from Sinonovacula constricta on RAW264.7 macrophage cells Liu, Zhidong Liu, Zhifang Li, Laihao Zhang, Junjie Zhao, Qiancheng Lin, Na Zhong, Wenzhu Jiang, Mei Food Sci Nutr Original Articles This study aimed to evaluate the immunomodulatory effect of the polysaccharide from Sinonovacula constricta (SCP‐1‐1) in RAW264.7 cells. SCP‐1‐1 with a molecular weight of 440.0 kDa consisted of glucose and mannose. The immunomodulatory assay results showed that SCP‐1‐1 could significantly enhance phagocytic ability, NO production, and some cytokines (TNF‐α, IL‐6, and IL‐1β) secretion of RAW264.7 cell in a dose‐dependent manner. Western blot analysis results demonstrated that SCP‐1‐1 could regulate the expression levels of the key proteins in the signaling pathways of RAW264.7 cell and might associated with NF‐κβ and PI3K signaling pathway. These findings could contribute to elucidate the immunomodulatory activities of the polysaccharide from Sinonovacula constricta. John Wiley and Sons Inc. 2022-01-25 /pmc/articles/PMC9007286/ /pubmed/35432971 http://dx.doi.org/10.1002/fsn3.2735 Text en © 2022 The Authors. Food Science & Nutrition published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Liu, Zhidong
Liu, Zhifang
Li, Laihao
Zhang, Junjie
Zhao, Qiancheng
Lin, Na
Zhong, Wenzhu
Jiang, Mei
Immunomodulatory effects of the polysaccharide from Sinonovacula constricta on RAW264.7 macrophage cells
title Immunomodulatory effects of the polysaccharide from Sinonovacula constricta on RAW264.7 macrophage cells
title_full Immunomodulatory effects of the polysaccharide from Sinonovacula constricta on RAW264.7 macrophage cells
title_fullStr Immunomodulatory effects of the polysaccharide from Sinonovacula constricta on RAW264.7 macrophage cells
title_full_unstemmed Immunomodulatory effects of the polysaccharide from Sinonovacula constricta on RAW264.7 macrophage cells
title_short Immunomodulatory effects of the polysaccharide from Sinonovacula constricta on RAW264.7 macrophage cells
title_sort immunomodulatory effects of the polysaccharide from sinonovacula constricta on raw264.7 macrophage cells
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9007286/
https://www.ncbi.nlm.nih.gov/pubmed/35432971
http://dx.doi.org/10.1002/fsn3.2735
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