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Study on Lung Injury Caused by Fine Particulate Matter and Intervention Effect of Rhodiola wallichiana
OBJECTIVE: The objective of this study was to observe the protective effect of Rhodiola wallichiana drops in a rat model of fine particulate matter (PM2.5) lung injury. METHODS: Forty male Wistar rats were randomly divided into blank control (NC), normal saline (NS), PM2.5-infected (PM), and Rhodiol...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9007651/ https://www.ncbi.nlm.nih.gov/pubmed/35432571 http://dx.doi.org/10.1155/2022/3693231 |
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author | Cao, Lei Lin, Hua Li, Qi Han, Shuzhi Yin, Hang Zhang, Ning Gao, Yanfeng Chen, Ye Ping, Fen |
author_facet | Cao, Lei Lin, Hua Li, Qi Han, Shuzhi Yin, Hang Zhang, Ning Gao, Yanfeng Chen, Ye Ping, Fen |
author_sort | Cao, Lei |
collection | PubMed |
description | OBJECTIVE: The objective of this study was to observe the protective effect of Rhodiola wallichiana drops in a rat model of fine particulate matter (PM2.5) lung injury. METHODS: Forty male Wistar rats were randomly divided into blank control (NC), normal saline (NS), PM2.5-infected (PM), and Rhodiola wallichiana (RW) groups. Rats in the NC group were not provided any interventions, whereas those in the NS and PM groups were administered normal saline and PM2.5 suspension by trachea drip once a week for four weeks. Rats in the RW group were intraperitoneally administered Rhodiola wallichiana for 14 days and then administered PM2.5 suspension by trachea drip 7 days after drug delivery. The levels of inflammatory factors such as interleukin-6, interleukin-1β, and tumor necrosis factor-alpha and oxidative stress biomarkers such as 8-hydroxy-2′-deoxyguanosine, 4-hydroxynonenal, and protein carbonyl content were determined in the serum and bronchoalveolar lavage fluid by ELISA. The level of 4-hydroxynonenal in the lung was also determined using Western blotting and immunohistochemical staining. RESULTS: Levels of inflammatory factors and oxidative stress biomarkers were all increased in the PM group but decreased in the RW group. Western blotting revealed increased 4-hydroxynonenal levels in the PM group but decreased levels in the RW group. Immunohistochemical staining also provided similar results. CONCLUSION: Rhodiola wallichiana could protect rats from inflammation and oxidative stress injury caused by PM2.5. |
format | Online Article Text |
id | pubmed-9007651 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-90076512022-04-14 Study on Lung Injury Caused by Fine Particulate Matter and Intervention Effect of Rhodiola wallichiana Cao, Lei Lin, Hua Li, Qi Han, Shuzhi Yin, Hang Zhang, Ning Gao, Yanfeng Chen, Ye Ping, Fen Evid Based Complement Alternat Med Research Article OBJECTIVE: The objective of this study was to observe the protective effect of Rhodiola wallichiana drops in a rat model of fine particulate matter (PM2.5) lung injury. METHODS: Forty male Wistar rats were randomly divided into blank control (NC), normal saline (NS), PM2.5-infected (PM), and Rhodiola wallichiana (RW) groups. Rats in the NC group were not provided any interventions, whereas those in the NS and PM groups were administered normal saline and PM2.5 suspension by trachea drip once a week for four weeks. Rats in the RW group were intraperitoneally administered Rhodiola wallichiana for 14 days and then administered PM2.5 suspension by trachea drip 7 days after drug delivery. The levels of inflammatory factors such as interleukin-6, interleukin-1β, and tumor necrosis factor-alpha and oxidative stress biomarkers such as 8-hydroxy-2′-deoxyguanosine, 4-hydroxynonenal, and protein carbonyl content were determined in the serum and bronchoalveolar lavage fluid by ELISA. The level of 4-hydroxynonenal in the lung was also determined using Western blotting and immunohistochemical staining. RESULTS: Levels of inflammatory factors and oxidative stress biomarkers were all increased in the PM group but decreased in the RW group. Western blotting revealed increased 4-hydroxynonenal levels in the PM group but decreased levels in the RW group. Immunohistochemical staining also provided similar results. CONCLUSION: Rhodiola wallichiana could protect rats from inflammation and oxidative stress injury caused by PM2.5. Hindawi 2022-04-06 /pmc/articles/PMC9007651/ /pubmed/35432571 http://dx.doi.org/10.1155/2022/3693231 Text en Copyright © 2022 Lei Cao et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Cao, Lei Lin, Hua Li, Qi Han, Shuzhi Yin, Hang Zhang, Ning Gao, Yanfeng Chen, Ye Ping, Fen Study on Lung Injury Caused by Fine Particulate Matter and Intervention Effect of Rhodiola wallichiana |
title | Study on Lung Injury Caused by Fine Particulate Matter and Intervention Effect of Rhodiola wallichiana |
title_full | Study on Lung Injury Caused by Fine Particulate Matter and Intervention Effect of Rhodiola wallichiana |
title_fullStr | Study on Lung Injury Caused by Fine Particulate Matter and Intervention Effect of Rhodiola wallichiana |
title_full_unstemmed | Study on Lung Injury Caused by Fine Particulate Matter and Intervention Effect of Rhodiola wallichiana |
title_short | Study on Lung Injury Caused by Fine Particulate Matter and Intervention Effect of Rhodiola wallichiana |
title_sort | study on lung injury caused by fine particulate matter and intervention effect of rhodiola wallichiana |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9007651/ https://www.ncbi.nlm.nih.gov/pubmed/35432571 http://dx.doi.org/10.1155/2022/3693231 |
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