Cargando…

Harnessing the Utility of Ex Vivo Patient Prostate Tissue Slice Cultures

Patient-derived prostate tissue explant cultures are powerful research tools that offer the potential for personalized medicine. These cultures preserve the local microenvironment of the surrounding stroma but are not without limitations and challenges. There are several methods and processing techn...

Descripción completa

Detalles Bibliográficos
Autores principales: Perez, Lillian M., Nonn, Larisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008363/
https://www.ncbi.nlm.nih.gov/pubmed/35433436
http://dx.doi.org/10.3389/fonc.2022.864723
_version_ 1784687036403286016
author Perez, Lillian M.
Nonn, Larisa
author_facet Perez, Lillian M.
Nonn, Larisa
author_sort Perez, Lillian M.
collection PubMed
description Patient-derived prostate tissue explant cultures are powerful research tools that offer the potential for personalized medicine. These cultures preserve the local microenvironment of the surrounding stroma but are not without limitations and challenges. There are several methods and processing techniques to culture tissue ex vivo, that include explant tissue chunks and precision-cut tissue slices. Precision-cut tissue slices provide a consistent distribution of nutrients and gases to the explant. Herein we summarize the prostate tissue slice method, its limitations and discuss the utility of this model, to investigate prostate biology and therapeutic treatment responses.
format Online
Article
Text
id pubmed-9008363
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-90083632022-04-15 Harnessing the Utility of Ex Vivo Patient Prostate Tissue Slice Cultures Perez, Lillian M. Nonn, Larisa Front Oncol Oncology Patient-derived prostate tissue explant cultures are powerful research tools that offer the potential for personalized medicine. These cultures preserve the local microenvironment of the surrounding stroma but are not without limitations and challenges. There are several methods and processing techniques to culture tissue ex vivo, that include explant tissue chunks and precision-cut tissue slices. Precision-cut tissue slices provide a consistent distribution of nutrients and gases to the explant. Herein we summarize the prostate tissue slice method, its limitations and discuss the utility of this model, to investigate prostate biology and therapeutic treatment responses. Frontiers Media S.A. 2022-03-31 /pmc/articles/PMC9008363/ /pubmed/35433436 http://dx.doi.org/10.3389/fonc.2022.864723 Text en Copyright © 2022 Perez and Nonn https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Perez, Lillian M.
Nonn, Larisa
Harnessing the Utility of Ex Vivo Patient Prostate Tissue Slice Cultures
title Harnessing the Utility of Ex Vivo Patient Prostate Tissue Slice Cultures
title_full Harnessing the Utility of Ex Vivo Patient Prostate Tissue Slice Cultures
title_fullStr Harnessing the Utility of Ex Vivo Patient Prostate Tissue Slice Cultures
title_full_unstemmed Harnessing the Utility of Ex Vivo Patient Prostate Tissue Slice Cultures
title_short Harnessing the Utility of Ex Vivo Patient Prostate Tissue Slice Cultures
title_sort harnessing the utility of ex vivo patient prostate tissue slice cultures
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008363/
https://www.ncbi.nlm.nih.gov/pubmed/35433436
http://dx.doi.org/10.3389/fonc.2022.864723
work_keys_str_mv AT perezlillianm harnessingtheutilityofexvivopatientprostatetissueslicecultures
AT nonnlarisa harnessingtheutilityofexvivopatientprostatetissueslicecultures