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Harnessing the Utility of Ex Vivo Patient Prostate Tissue Slice Cultures
Patient-derived prostate tissue explant cultures are powerful research tools that offer the potential for personalized medicine. These cultures preserve the local microenvironment of the surrounding stroma but are not without limitations and challenges. There are several methods and processing techn...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008363/ https://www.ncbi.nlm.nih.gov/pubmed/35433436 http://dx.doi.org/10.3389/fonc.2022.864723 |
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author | Perez, Lillian M. Nonn, Larisa |
author_facet | Perez, Lillian M. Nonn, Larisa |
author_sort | Perez, Lillian M. |
collection | PubMed |
description | Patient-derived prostate tissue explant cultures are powerful research tools that offer the potential for personalized medicine. These cultures preserve the local microenvironment of the surrounding stroma but are not without limitations and challenges. There are several methods and processing techniques to culture tissue ex vivo, that include explant tissue chunks and precision-cut tissue slices. Precision-cut tissue slices provide a consistent distribution of nutrients and gases to the explant. Herein we summarize the prostate tissue slice method, its limitations and discuss the utility of this model, to investigate prostate biology and therapeutic treatment responses. |
format | Online Article Text |
id | pubmed-9008363 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-90083632022-04-15 Harnessing the Utility of Ex Vivo Patient Prostate Tissue Slice Cultures Perez, Lillian M. Nonn, Larisa Front Oncol Oncology Patient-derived prostate tissue explant cultures are powerful research tools that offer the potential for personalized medicine. These cultures preserve the local microenvironment of the surrounding stroma but are not without limitations and challenges. There are several methods and processing techniques to culture tissue ex vivo, that include explant tissue chunks and precision-cut tissue slices. Precision-cut tissue slices provide a consistent distribution of nutrients and gases to the explant. Herein we summarize the prostate tissue slice method, its limitations and discuss the utility of this model, to investigate prostate biology and therapeutic treatment responses. Frontiers Media S.A. 2022-03-31 /pmc/articles/PMC9008363/ /pubmed/35433436 http://dx.doi.org/10.3389/fonc.2022.864723 Text en Copyright © 2022 Perez and Nonn https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Oncology Perez, Lillian M. Nonn, Larisa Harnessing the Utility of Ex Vivo Patient Prostate Tissue Slice Cultures |
title | Harnessing the Utility of Ex Vivo Patient Prostate Tissue Slice Cultures |
title_full | Harnessing the Utility of Ex Vivo Patient Prostate Tissue Slice Cultures |
title_fullStr | Harnessing the Utility of Ex Vivo Patient Prostate Tissue Slice Cultures |
title_full_unstemmed | Harnessing the Utility of Ex Vivo Patient Prostate Tissue Slice Cultures |
title_short | Harnessing the Utility of Ex Vivo Patient Prostate Tissue Slice Cultures |
title_sort | harnessing the utility of ex vivo patient prostate tissue slice cultures |
topic | Oncology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008363/ https://www.ncbi.nlm.nih.gov/pubmed/35433436 http://dx.doi.org/10.3389/fonc.2022.864723 |
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