Cargando…

Assessing Temporal Changes in Microbial Communities in Hyalomma dromedarii Collected From Camels in the UAE Using High-Throughput Sequencing

Ticks (Acari) are ectoparasites of animals that harbor communities of microbes of importance to animal and human health. Microbial communities associated with ticks exhibit temporal patterns of variation in their composition, with different genera dominating at different times of the year. In this s...

Descripción completa

Detalles Bibliográficos
Autores principales: Perveen, Nighat, Muzaffar, Sabir Bin, Vijayan, Ranjit, Al-Deeb, Mohammad Ali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008585/
https://www.ncbi.nlm.nih.gov/pubmed/35433895
http://dx.doi.org/10.3389/fvets.2022.861233
_version_ 1784687085420019712
author Perveen, Nighat
Muzaffar, Sabir Bin
Vijayan, Ranjit
Al-Deeb, Mohammad Ali
author_facet Perveen, Nighat
Muzaffar, Sabir Bin
Vijayan, Ranjit
Al-Deeb, Mohammad Ali
author_sort Perveen, Nighat
collection PubMed
description Ticks (Acari) are ectoparasites of animals that harbor communities of microbes of importance to animal and human health. Microbial communities associated with ticks exhibit temporal patterns of variation in their composition, with different genera dominating at different times of the year. In this study, molecular tools were used to assess the composition of the microbial communities associated with Hyalomma dromdarii. Adult ticks were collected every month for 1 year from 25 camels in the UAE. A total of 12 DNA pools were prepared (one pool for each month). We monitored the microbiota of ticks using high-throughput sequencing of the V3–V4 region of the bacterial 16S rRNA gene. A total of 614 operational taxonomic units were produced through de novo clustering and belonged to 17 phyla, 30 classes, 46 orders, 118 families, and 222 genera. Fifteen bacterial families were found to be the most abundant. The dominant bacterial communities associated with H. dromedarii belonged to the genera Staphylococcus, Bacillus, Francisella, and Corynebacterium, which were reported with high relative abundance from all months. No significant correlation occurred between the abundance of microbial families or genera in H. dromedarii ticks and the ambient temperature. Our findings revealed, for the first time in the UAE, temporal fluctuations of microbial communities in H. dromedarii ticks and provided key insights on the interaction between different microbial groups. Moreover, our results contribute to the current understanding of disease development and allow more investigations for potentially pathogenic microbiota.
format Online
Article
Text
id pubmed-9008585
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-90085852022-04-15 Assessing Temporal Changes in Microbial Communities in Hyalomma dromedarii Collected From Camels in the UAE Using High-Throughput Sequencing Perveen, Nighat Muzaffar, Sabir Bin Vijayan, Ranjit Al-Deeb, Mohammad Ali Front Vet Sci Veterinary Science Ticks (Acari) are ectoparasites of animals that harbor communities of microbes of importance to animal and human health. Microbial communities associated with ticks exhibit temporal patterns of variation in their composition, with different genera dominating at different times of the year. In this study, molecular tools were used to assess the composition of the microbial communities associated with Hyalomma dromdarii. Adult ticks were collected every month for 1 year from 25 camels in the UAE. A total of 12 DNA pools were prepared (one pool for each month). We monitored the microbiota of ticks using high-throughput sequencing of the V3–V4 region of the bacterial 16S rRNA gene. A total of 614 operational taxonomic units were produced through de novo clustering and belonged to 17 phyla, 30 classes, 46 orders, 118 families, and 222 genera. Fifteen bacterial families were found to be the most abundant. The dominant bacterial communities associated with H. dromedarii belonged to the genera Staphylococcus, Bacillus, Francisella, and Corynebacterium, which were reported with high relative abundance from all months. No significant correlation occurred between the abundance of microbial families or genera in H. dromedarii ticks and the ambient temperature. Our findings revealed, for the first time in the UAE, temporal fluctuations of microbial communities in H. dromedarii ticks and provided key insights on the interaction between different microbial groups. Moreover, our results contribute to the current understanding of disease development and allow more investigations for potentially pathogenic microbiota. Frontiers Media S.A. 2022-03-31 /pmc/articles/PMC9008585/ /pubmed/35433895 http://dx.doi.org/10.3389/fvets.2022.861233 Text en Copyright © 2022 Perveen, Muzaffar, Vijayan and Al-Deeb. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Perveen, Nighat
Muzaffar, Sabir Bin
Vijayan, Ranjit
Al-Deeb, Mohammad Ali
Assessing Temporal Changes in Microbial Communities in Hyalomma dromedarii Collected From Camels in the UAE Using High-Throughput Sequencing
title Assessing Temporal Changes in Microbial Communities in Hyalomma dromedarii Collected From Camels in the UAE Using High-Throughput Sequencing
title_full Assessing Temporal Changes in Microbial Communities in Hyalomma dromedarii Collected From Camels in the UAE Using High-Throughput Sequencing
title_fullStr Assessing Temporal Changes in Microbial Communities in Hyalomma dromedarii Collected From Camels in the UAE Using High-Throughput Sequencing
title_full_unstemmed Assessing Temporal Changes in Microbial Communities in Hyalomma dromedarii Collected From Camels in the UAE Using High-Throughput Sequencing
title_short Assessing Temporal Changes in Microbial Communities in Hyalomma dromedarii Collected From Camels in the UAE Using High-Throughput Sequencing
title_sort assessing temporal changes in microbial communities in hyalomma dromedarii collected from camels in the uae using high-throughput sequencing
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008585/
https://www.ncbi.nlm.nih.gov/pubmed/35433895
http://dx.doi.org/10.3389/fvets.2022.861233
work_keys_str_mv AT perveennighat assessingtemporalchangesinmicrobialcommunitiesinhyalommadromedariicollectedfromcamelsintheuaeusinghighthroughputsequencing
AT muzaffarsabirbin assessingtemporalchangesinmicrobialcommunitiesinhyalommadromedariicollectedfromcamelsintheuaeusinghighthroughputsequencing
AT vijayanranjit assessingtemporalchangesinmicrobialcommunitiesinhyalommadromedariicollectedfromcamelsintheuaeusinghighthroughputsequencing
AT aldeebmohammadali assessingtemporalchangesinmicrobialcommunitiesinhyalommadromedariicollectedfromcamelsintheuaeusinghighthroughputsequencing