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SUMO1 Promotes Mesangial Cell Proliferation Through Inhibiting Autophagy in a Cell Model of IgA Nephropathy

IgA nephropathy (IgAN) is a common form of primary glomerulonephritis and its main pathological changes are mesangial cell proliferation and matrix expansion. Autophagy inhibition may result in its mesangial cell proliferation and renal lesions. SUMOylation is a eukaryotic-reversible post-translatio...

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Autores principales: Tan, Xia, Liu, Yexin, Liu, Di, Tang, Xiaofang, Xia, Ming, Chen, Guochun, He, Liyu, Zhu, Xuejing, Liu, Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008726/
https://www.ncbi.nlm.nih.gov/pubmed/35433764
http://dx.doi.org/10.3389/fmed.2022.834164
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author Tan, Xia
Liu, Yexin
Liu, Di
Tang, Xiaofang
Xia, Ming
Chen, Guochun
He, Liyu
Zhu, Xuejing
Liu, Hong
author_facet Tan, Xia
Liu, Yexin
Liu, Di
Tang, Xiaofang
Xia, Ming
Chen, Guochun
He, Liyu
Zhu, Xuejing
Liu, Hong
author_sort Tan, Xia
collection PubMed
description IgA nephropathy (IgAN) is a common form of primary glomerulonephritis and its main pathological changes are mesangial cell proliferation and matrix expansion. Autophagy inhibition may result in its mesangial cell proliferation and renal lesions. SUMOylation is a eukaryotic-reversible post-translational modification where SUMO is covalently attached to target proteins to regulate their properties. It is largely unclear whether SUMOylation contributes to the pathogenesis of IgAN. This study was designed to investigate the change of protein SUMO1 in mesangial cells of IgAN and its association with autophagy. We found the expression of SUMO1 was upregulated in IgAN, IgA mouse model, and aIgA1-stimulated mesangial cells. In aIgA1-stimulated mesangial cell model, we tested LC3II/I and p62, the autophagy-related proteins suggested the inhibition of autophagy. Inhibited SUMOylation with ginkgolic acid (GA) or silencing SUMO1 could downregulate SUMO1 and SUMO1-p53, promote autophagy, and lessen cell proliferation. In summary, in the mesangial cells stimulated with aIgA1, SUMO1 may contribute to its cell proliferation through inhibited autophagy, and SUMO1-p53 may play a role in this process.
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spelling pubmed-90087262022-04-15 SUMO1 Promotes Mesangial Cell Proliferation Through Inhibiting Autophagy in a Cell Model of IgA Nephropathy Tan, Xia Liu, Yexin Liu, Di Tang, Xiaofang Xia, Ming Chen, Guochun He, Liyu Zhu, Xuejing Liu, Hong Front Med (Lausanne) Medicine IgA nephropathy (IgAN) is a common form of primary glomerulonephritis and its main pathological changes are mesangial cell proliferation and matrix expansion. Autophagy inhibition may result in its mesangial cell proliferation and renal lesions. SUMOylation is a eukaryotic-reversible post-translational modification where SUMO is covalently attached to target proteins to regulate their properties. It is largely unclear whether SUMOylation contributes to the pathogenesis of IgAN. This study was designed to investigate the change of protein SUMO1 in mesangial cells of IgAN and its association with autophagy. We found the expression of SUMO1 was upregulated in IgAN, IgA mouse model, and aIgA1-stimulated mesangial cells. In aIgA1-stimulated mesangial cell model, we tested LC3II/I and p62, the autophagy-related proteins suggested the inhibition of autophagy. Inhibited SUMOylation with ginkgolic acid (GA) or silencing SUMO1 could downregulate SUMO1 and SUMO1-p53, promote autophagy, and lessen cell proliferation. In summary, in the mesangial cells stimulated with aIgA1, SUMO1 may contribute to its cell proliferation through inhibited autophagy, and SUMO1-p53 may play a role in this process. Frontiers Media S.A. 2022-03-31 /pmc/articles/PMC9008726/ /pubmed/35433764 http://dx.doi.org/10.3389/fmed.2022.834164 Text en Copyright © 2022 Tan, Liu, Liu, Tang, Xia, Chen, He, Zhu and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Medicine
Tan, Xia
Liu, Yexin
Liu, Di
Tang, Xiaofang
Xia, Ming
Chen, Guochun
He, Liyu
Zhu, Xuejing
Liu, Hong
SUMO1 Promotes Mesangial Cell Proliferation Through Inhibiting Autophagy in a Cell Model of IgA Nephropathy
title SUMO1 Promotes Mesangial Cell Proliferation Through Inhibiting Autophagy in a Cell Model of IgA Nephropathy
title_full SUMO1 Promotes Mesangial Cell Proliferation Through Inhibiting Autophagy in a Cell Model of IgA Nephropathy
title_fullStr SUMO1 Promotes Mesangial Cell Proliferation Through Inhibiting Autophagy in a Cell Model of IgA Nephropathy
title_full_unstemmed SUMO1 Promotes Mesangial Cell Proliferation Through Inhibiting Autophagy in a Cell Model of IgA Nephropathy
title_short SUMO1 Promotes Mesangial Cell Proliferation Through Inhibiting Autophagy in a Cell Model of IgA Nephropathy
title_sort sumo1 promotes mesangial cell proliferation through inhibiting autophagy in a cell model of iga nephropathy
topic Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008726/
https://www.ncbi.nlm.nih.gov/pubmed/35433764
http://dx.doi.org/10.3389/fmed.2022.834164
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