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Omics Profiles of Non-transgenic Scion Grafted on Transgenic RdDM Rootstock

Grafting of commercial varieties onto transgenic stress-tolerant rootstocks is attractive approach, because fruit from the non-transgenic plant body does not contain foreign genes. RNA silencing can modulate gene expression and protect host plants from viruses and insects, and small RNAs (sRNAs), ke...

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Autores principales: Kodama, Hiroaki, Umeyama, Yukiko, Miyahara, Taira, Oguchi, Taichi, Tsujimoto, Takashi, Ozeki, Yoshihiro, Ogawa, Takumi, Yamaguchi, Yube, Ohta, Daisaku
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Food Safety Commission, Cabinet Office, Government of Japan 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008877/
https://www.ncbi.nlm.nih.gov/pubmed/35510071
http://dx.doi.org/10.14252/foodsafetyfscj.D-21-00012
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author Kodama, Hiroaki
Umeyama, Yukiko
Miyahara, Taira
Oguchi, Taichi
Tsujimoto, Takashi
Ozeki, Yoshihiro
Ogawa, Takumi
Yamaguchi, Yube
Ohta, Daisaku
author_facet Kodama, Hiroaki
Umeyama, Yukiko
Miyahara, Taira
Oguchi, Taichi
Tsujimoto, Takashi
Ozeki, Yoshihiro
Ogawa, Takumi
Yamaguchi, Yube
Ohta, Daisaku
author_sort Kodama, Hiroaki
collection PubMed
description Grafting of commercial varieties onto transgenic stress-tolerant rootstocks is attractive approach, because fruit from the non-transgenic plant body does not contain foreign genes. RNA silencing can modulate gene expression and protect host plants from viruses and insects, and small RNAs (sRNAs), key molecules of RNA silencing, can move systemically. Here, to evaluate the safety of foods obtained from sRNA-recipient plant bodies, we investigated the effects of rootstock-derived sRNAs involved in mediating RNA-directed DNA methylation (RdDM) on non-transgenic scions. We used tobacco rootstocks showing RdDM against the cauliflower mosaic virus (CaMV) 35S promoter. When scions harboring CaMV 35S promoter sequence were grafted onto RdDM-inducing rootstocks, we found that RdDM-inducing sRNAs were only weakly transported from the rootstocks to the scion, and we observed a low level of DNA methylation of the CaMV 35S promoter in the scion. Next, wild-type (WT) tobacco scions were grafted onto RdDM-inducing rootstocks (designated NT) or WT rootstocks (designated NN), and scion leaves were subjected to multi-omics analyses. Our transcriptomic analysis detected 55 differentially expressed genes between the NT and NN samples. A principal component analysis of proteome profiles showed no significant differences. In the positive and negative modes of LC-ESI-MS and GC-EI-MS analyses, we found a large overlap between the metabolomic clusters of the NT and NN samples. In contrast, the negative mode of a LC-ESI-MS analysis showed separation of clusters of NT and NN metabolites, and we detected 6 peak groups that significantly differed. In conclusion, we found that grafting onto RdDM-inducing rootstocks caused a low-level transmission of sRNAs, resulting in limited DNA methylation in the scion. However, the causal relationships between sRNA transmission and the very slight changes in the transcriptomic and metabolomic profiles of the scions remains unclear. The safety assessment points for grafting with RdDM rootstocks are discussed.
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spelling pubmed-90088772022-05-03 Omics Profiles of Non-transgenic Scion Grafted on Transgenic RdDM Rootstock Kodama, Hiroaki Umeyama, Yukiko Miyahara, Taira Oguchi, Taichi Tsujimoto, Takashi Ozeki, Yoshihiro Ogawa, Takumi Yamaguchi, Yube Ohta, Daisaku Food Saf (Tokyo) Original Article Grafting of commercial varieties onto transgenic stress-tolerant rootstocks is attractive approach, because fruit from the non-transgenic plant body does not contain foreign genes. RNA silencing can modulate gene expression and protect host plants from viruses and insects, and small RNAs (sRNAs), key molecules of RNA silencing, can move systemically. Here, to evaluate the safety of foods obtained from sRNA-recipient plant bodies, we investigated the effects of rootstock-derived sRNAs involved in mediating RNA-directed DNA methylation (RdDM) on non-transgenic scions. We used tobacco rootstocks showing RdDM against the cauliflower mosaic virus (CaMV) 35S promoter. When scions harboring CaMV 35S promoter sequence were grafted onto RdDM-inducing rootstocks, we found that RdDM-inducing sRNAs were only weakly transported from the rootstocks to the scion, and we observed a low level of DNA methylation of the CaMV 35S promoter in the scion. Next, wild-type (WT) tobacco scions were grafted onto RdDM-inducing rootstocks (designated NT) or WT rootstocks (designated NN), and scion leaves were subjected to multi-omics analyses. Our transcriptomic analysis detected 55 differentially expressed genes between the NT and NN samples. A principal component analysis of proteome profiles showed no significant differences. In the positive and negative modes of LC-ESI-MS and GC-EI-MS analyses, we found a large overlap between the metabolomic clusters of the NT and NN samples. In contrast, the negative mode of a LC-ESI-MS analysis showed separation of clusters of NT and NN metabolites, and we detected 6 peak groups that significantly differed. In conclusion, we found that grafting onto RdDM-inducing rootstocks caused a low-level transmission of sRNAs, resulting in limited DNA methylation in the scion. However, the causal relationships between sRNA transmission and the very slight changes in the transcriptomic and metabolomic profiles of the scions remains unclear. The safety assessment points for grafting with RdDM rootstocks are discussed. Food Safety Commission, Cabinet Office, Government of Japan 2022-03-04 /pmc/articles/PMC9008877/ /pubmed/35510071 http://dx.doi.org/10.14252/foodsafetyfscj.D-21-00012 Text en ©2022 Food Safety Commission, Cabinet Office, Government of Japan https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY) 4.0 License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kodama, Hiroaki
Umeyama, Yukiko
Miyahara, Taira
Oguchi, Taichi
Tsujimoto, Takashi
Ozeki, Yoshihiro
Ogawa, Takumi
Yamaguchi, Yube
Ohta, Daisaku
Omics Profiles of Non-transgenic Scion Grafted on Transgenic RdDM Rootstock
title Omics Profiles of Non-transgenic Scion Grafted on Transgenic RdDM Rootstock
title_full Omics Profiles of Non-transgenic Scion Grafted on Transgenic RdDM Rootstock
title_fullStr Omics Profiles of Non-transgenic Scion Grafted on Transgenic RdDM Rootstock
title_full_unstemmed Omics Profiles of Non-transgenic Scion Grafted on Transgenic RdDM Rootstock
title_short Omics Profiles of Non-transgenic Scion Grafted on Transgenic RdDM Rootstock
title_sort omics profiles of non-transgenic scion grafted on transgenic rddm rootstock
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008877/
https://www.ncbi.nlm.nih.gov/pubmed/35510071
http://dx.doi.org/10.14252/foodsafetyfscj.D-21-00012
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