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Construction of Mussel-Inspired Dopamine–Zn(2+) Coating on Titanium Oxide Nanotubes to Improve Hemocompatibility, Cytocompatibility, and Antibacterial Activity

Zinc ions (Zn(2+)) are a highly potent bioactive factor with a broad spectrum of physiological functions. In situ continuous and controllable release of Zn(2+) from the biomaterials can effectively improve the biocompatibility and antibacterial activity. In the present study, inspired by the adhesio...

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Autores principales: Hu, Youdong, Zhou, Hualan, Liu, Tingting, Yang, Minhui, Zhang, Qiuyang, Pan, Changjiang, Lin, Jiafeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9009227/
https://www.ncbi.nlm.nih.gov/pubmed/35433663
http://dx.doi.org/10.3389/fbioe.2022.884258
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author Hu, Youdong
Zhou, Hualan
Liu, Tingting
Yang, Minhui
Zhang, Qiuyang
Pan, Changjiang
Lin, Jiafeng
author_facet Hu, Youdong
Zhou, Hualan
Liu, Tingting
Yang, Minhui
Zhang, Qiuyang
Pan, Changjiang
Lin, Jiafeng
author_sort Hu, Youdong
collection PubMed
description Zinc ions (Zn(2+)) are a highly potent bioactive factor with a broad spectrum of physiological functions. In situ continuous and controllable release of Zn(2+) from the biomaterials can effectively improve the biocompatibility and antibacterial activity. In the present study, inspired by the adhesion and protein cross-linking in the mussel byssus, with the aim of improving the biocompatibility of titanium, a cost-effective one-step metal–catecholamine assembly strategy was developed to prepare a biomimetic dopamine–Zn(2+) (DA-Zn(2+)) coating by immersing the titanium oxide nanotube (TNT) arrays on the titanium surface prepared by anodic oxidation into an aqueous solution containing dopamine (DA) and zinc ions (Zn(2+)). The DA-Zn(2+) coatings with the different zinc contents exhibited excellent hydrophilicity. Due to the continuous release of zinc ions from the DA-Zn(2+) coating, the coated titanium oxide nanotubes displayed excellent hemocompatibility characterized by platelet adhesion and activation and hemolysis assay. Moreover, the DA-Zn(2+)-coated samples exhibited an excellent ability to enhance endothelial cell (EC) adhesion and proliferation. In addition, the DA-Zn(2+) coating can also enhance the antibacterial activity of the nanotubes. Therefore, long-term in situ Zn(2+)-releasing coating of the present study could serve as the bio-surfaces for long-term prevention of thrombosis, improvement of cytocompatibility to endothelial cells, and antibacterial activity. Due to the easy operation and strong binding ability of the polydopamine on various complicated shapes, the method of the present study can be further applied to other blood contact biomaterials or implantable medical devices to improve the biocompatibility.
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spelling pubmed-90092272022-04-15 Construction of Mussel-Inspired Dopamine–Zn(2+) Coating on Titanium Oxide Nanotubes to Improve Hemocompatibility, Cytocompatibility, and Antibacterial Activity Hu, Youdong Zhou, Hualan Liu, Tingting Yang, Minhui Zhang, Qiuyang Pan, Changjiang Lin, Jiafeng Front Bioeng Biotechnol Bioengineering and Biotechnology Zinc ions (Zn(2+)) are a highly potent bioactive factor with a broad spectrum of physiological functions. In situ continuous and controllable release of Zn(2+) from the biomaterials can effectively improve the biocompatibility and antibacterial activity. In the present study, inspired by the adhesion and protein cross-linking in the mussel byssus, with the aim of improving the biocompatibility of titanium, a cost-effective one-step metal–catecholamine assembly strategy was developed to prepare a biomimetic dopamine–Zn(2+) (DA-Zn(2+)) coating by immersing the titanium oxide nanotube (TNT) arrays on the titanium surface prepared by anodic oxidation into an aqueous solution containing dopamine (DA) and zinc ions (Zn(2+)). The DA-Zn(2+) coatings with the different zinc contents exhibited excellent hydrophilicity. Due to the continuous release of zinc ions from the DA-Zn(2+) coating, the coated titanium oxide nanotubes displayed excellent hemocompatibility characterized by platelet adhesion and activation and hemolysis assay. Moreover, the DA-Zn(2+)-coated samples exhibited an excellent ability to enhance endothelial cell (EC) adhesion and proliferation. In addition, the DA-Zn(2+) coating can also enhance the antibacterial activity of the nanotubes. Therefore, long-term in situ Zn(2+)-releasing coating of the present study could serve as the bio-surfaces for long-term prevention of thrombosis, improvement of cytocompatibility to endothelial cells, and antibacterial activity. Due to the easy operation and strong binding ability of the polydopamine on various complicated shapes, the method of the present study can be further applied to other blood contact biomaterials or implantable medical devices to improve the biocompatibility. Frontiers Media S.A. 2022-03-31 /pmc/articles/PMC9009227/ /pubmed/35433663 http://dx.doi.org/10.3389/fbioe.2022.884258 Text en Copyright © 2022 Hu, Zhou, Liu, Yang, Zhang, Pan and Lin. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Hu, Youdong
Zhou, Hualan
Liu, Tingting
Yang, Minhui
Zhang, Qiuyang
Pan, Changjiang
Lin, Jiafeng
Construction of Mussel-Inspired Dopamine–Zn(2+) Coating on Titanium Oxide Nanotubes to Improve Hemocompatibility, Cytocompatibility, and Antibacterial Activity
title Construction of Mussel-Inspired Dopamine–Zn(2+) Coating on Titanium Oxide Nanotubes to Improve Hemocompatibility, Cytocompatibility, and Antibacterial Activity
title_full Construction of Mussel-Inspired Dopamine–Zn(2+) Coating on Titanium Oxide Nanotubes to Improve Hemocompatibility, Cytocompatibility, and Antibacterial Activity
title_fullStr Construction of Mussel-Inspired Dopamine–Zn(2+) Coating on Titanium Oxide Nanotubes to Improve Hemocompatibility, Cytocompatibility, and Antibacterial Activity
title_full_unstemmed Construction of Mussel-Inspired Dopamine–Zn(2+) Coating on Titanium Oxide Nanotubes to Improve Hemocompatibility, Cytocompatibility, and Antibacterial Activity
title_short Construction of Mussel-Inspired Dopamine–Zn(2+) Coating on Titanium Oxide Nanotubes to Improve Hemocompatibility, Cytocompatibility, and Antibacterial Activity
title_sort construction of mussel-inspired dopamine–zn(2+) coating on titanium oxide nanotubes to improve hemocompatibility, cytocompatibility, and antibacterial activity
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9009227/
https://www.ncbi.nlm.nih.gov/pubmed/35433663
http://dx.doi.org/10.3389/fbioe.2022.884258
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