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Nexmifa Regulates Axon Morphogenesis in Motor Neurons in Zebrafish

Nexmif is mainly expressed in the central nervous system (CNS) and plays important roles in cell migration, cell to cell and cell-matrix adhesion, and maintains normal synaptic formation and function. Nevertheless, it is unclear how nexmif is linked to motor neuron morphogenesis. Here, we provided i...

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Autores principales: Zheng, Yu-qin, Suo, Gui-hai, Liu, Dong, Li, Hai-ying, Wu, You-jia, Ni, Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9009263/
https://www.ncbi.nlm.nih.gov/pubmed/35431796
http://dx.doi.org/10.3389/fnmol.2022.848257
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author Zheng, Yu-qin
Suo, Gui-hai
Liu, Dong
Li, Hai-ying
Wu, You-jia
Ni, Hong
author_facet Zheng, Yu-qin
Suo, Gui-hai
Liu, Dong
Li, Hai-ying
Wu, You-jia
Ni, Hong
author_sort Zheng, Yu-qin
collection PubMed
description Nexmif is mainly expressed in the central nervous system (CNS) and plays important roles in cell migration, cell to cell and cell-matrix adhesion, and maintains normal synaptic formation and function. Nevertheless, it is unclear how nexmif is linked to motor neuron morphogenesis. Here, we provided in situ hybridization evidence that nexmifa (zebrafish paralog) was localized to the brain and spinal cord and acted as a vital regulator of motor neuron morphogenesis. Nexmifa deficiency in zebrafish larvae generated abnormal primary motor neuron (PMN) development, including truncated Cap axons and decreased branches in Cap axons. Importantly, RNA-sequencing showed that nexmifa-depleted zebrafish embryos caused considerable CNS related gene expression alterations. Differentially expressed genes (DEGs) were mainly involved in axon guidance and several synaptic pathways, including glutamatergic, GABAergic, dopaminergic, cholinergic, and serotonergic synapse pathways, according to Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway annotation. In particular, when compared with other pathways, DEGs were highest (84) in the axon guidance pathway, according to Organismal Systems. Efna5b, bmpr2b, and sema6ba were decreased markedly in nexmifa-depleted zebrafish embryos. Moreover, both overexpression of efna5b mRNA and sema6ba mRNA could partially rescued motor neurons morphogenesis. These observations supported nexmifa as regulating axon morphogenesis of motor neurons in zebrafish. Taken together, nexmifa elicited crucial roles during motor neuron development by regulating the morphology of neuronal axons.
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spelling pubmed-90092632022-04-15 Nexmifa Regulates Axon Morphogenesis in Motor Neurons in Zebrafish Zheng, Yu-qin Suo, Gui-hai Liu, Dong Li, Hai-ying Wu, You-jia Ni, Hong Front Mol Neurosci Neuroscience Nexmif is mainly expressed in the central nervous system (CNS) and plays important roles in cell migration, cell to cell and cell-matrix adhesion, and maintains normal synaptic formation and function. Nevertheless, it is unclear how nexmif is linked to motor neuron morphogenesis. Here, we provided in situ hybridization evidence that nexmifa (zebrafish paralog) was localized to the brain and spinal cord and acted as a vital regulator of motor neuron morphogenesis. Nexmifa deficiency in zebrafish larvae generated abnormal primary motor neuron (PMN) development, including truncated Cap axons and decreased branches in Cap axons. Importantly, RNA-sequencing showed that nexmifa-depleted zebrafish embryos caused considerable CNS related gene expression alterations. Differentially expressed genes (DEGs) were mainly involved in axon guidance and several synaptic pathways, including glutamatergic, GABAergic, dopaminergic, cholinergic, and serotonergic synapse pathways, according to Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway annotation. In particular, when compared with other pathways, DEGs were highest (84) in the axon guidance pathway, according to Organismal Systems. Efna5b, bmpr2b, and sema6ba were decreased markedly in nexmifa-depleted zebrafish embryos. Moreover, both overexpression of efna5b mRNA and sema6ba mRNA could partially rescued motor neurons morphogenesis. These observations supported nexmifa as regulating axon morphogenesis of motor neurons in zebrafish. Taken together, nexmifa elicited crucial roles during motor neuron development by regulating the morphology of neuronal axons. Frontiers Media S.A. 2022-03-31 /pmc/articles/PMC9009263/ /pubmed/35431796 http://dx.doi.org/10.3389/fnmol.2022.848257 Text en Copyright © 2022 Zheng, Suo, Liu, Li, Wu and Ni. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Zheng, Yu-qin
Suo, Gui-hai
Liu, Dong
Li, Hai-ying
Wu, You-jia
Ni, Hong
Nexmifa Regulates Axon Morphogenesis in Motor Neurons in Zebrafish
title Nexmifa Regulates Axon Morphogenesis in Motor Neurons in Zebrafish
title_full Nexmifa Regulates Axon Morphogenesis in Motor Neurons in Zebrafish
title_fullStr Nexmifa Regulates Axon Morphogenesis in Motor Neurons in Zebrafish
title_full_unstemmed Nexmifa Regulates Axon Morphogenesis in Motor Neurons in Zebrafish
title_short Nexmifa Regulates Axon Morphogenesis in Motor Neurons in Zebrafish
title_sort nexmifa regulates axon morphogenesis in motor neurons in zebrafish
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9009263/
https://www.ncbi.nlm.nih.gov/pubmed/35431796
http://dx.doi.org/10.3389/fnmol.2022.848257
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