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Nexmifa Regulates Axon Morphogenesis in Motor Neurons in Zebrafish
Nexmif is mainly expressed in the central nervous system (CNS) and plays important roles in cell migration, cell to cell and cell-matrix adhesion, and maintains normal synaptic formation and function. Nevertheless, it is unclear how nexmif is linked to motor neuron morphogenesis. Here, we provided i...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9009263/ https://www.ncbi.nlm.nih.gov/pubmed/35431796 http://dx.doi.org/10.3389/fnmol.2022.848257 |
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author | Zheng, Yu-qin Suo, Gui-hai Liu, Dong Li, Hai-ying Wu, You-jia Ni, Hong |
author_facet | Zheng, Yu-qin Suo, Gui-hai Liu, Dong Li, Hai-ying Wu, You-jia Ni, Hong |
author_sort | Zheng, Yu-qin |
collection | PubMed |
description | Nexmif is mainly expressed in the central nervous system (CNS) and plays important roles in cell migration, cell to cell and cell-matrix adhesion, and maintains normal synaptic formation and function. Nevertheless, it is unclear how nexmif is linked to motor neuron morphogenesis. Here, we provided in situ hybridization evidence that nexmifa (zebrafish paralog) was localized to the brain and spinal cord and acted as a vital regulator of motor neuron morphogenesis. Nexmifa deficiency in zebrafish larvae generated abnormal primary motor neuron (PMN) development, including truncated Cap axons and decreased branches in Cap axons. Importantly, RNA-sequencing showed that nexmifa-depleted zebrafish embryos caused considerable CNS related gene expression alterations. Differentially expressed genes (DEGs) were mainly involved in axon guidance and several synaptic pathways, including glutamatergic, GABAergic, dopaminergic, cholinergic, and serotonergic synapse pathways, according to Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway annotation. In particular, when compared with other pathways, DEGs were highest (84) in the axon guidance pathway, according to Organismal Systems. Efna5b, bmpr2b, and sema6ba were decreased markedly in nexmifa-depleted zebrafish embryos. Moreover, both overexpression of efna5b mRNA and sema6ba mRNA could partially rescued motor neurons morphogenesis. These observations supported nexmifa as regulating axon morphogenesis of motor neurons in zebrafish. Taken together, nexmifa elicited crucial roles during motor neuron development by regulating the morphology of neuronal axons. |
format | Online Article Text |
id | pubmed-9009263 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-90092632022-04-15 Nexmifa Regulates Axon Morphogenesis in Motor Neurons in Zebrafish Zheng, Yu-qin Suo, Gui-hai Liu, Dong Li, Hai-ying Wu, You-jia Ni, Hong Front Mol Neurosci Neuroscience Nexmif is mainly expressed in the central nervous system (CNS) and plays important roles in cell migration, cell to cell and cell-matrix adhesion, and maintains normal synaptic formation and function. Nevertheless, it is unclear how nexmif is linked to motor neuron morphogenesis. Here, we provided in situ hybridization evidence that nexmifa (zebrafish paralog) was localized to the brain and spinal cord and acted as a vital regulator of motor neuron morphogenesis. Nexmifa deficiency in zebrafish larvae generated abnormal primary motor neuron (PMN) development, including truncated Cap axons and decreased branches in Cap axons. Importantly, RNA-sequencing showed that nexmifa-depleted zebrafish embryos caused considerable CNS related gene expression alterations. Differentially expressed genes (DEGs) were mainly involved in axon guidance and several synaptic pathways, including glutamatergic, GABAergic, dopaminergic, cholinergic, and serotonergic synapse pathways, according to Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway annotation. In particular, when compared with other pathways, DEGs were highest (84) in the axon guidance pathway, according to Organismal Systems. Efna5b, bmpr2b, and sema6ba were decreased markedly in nexmifa-depleted zebrafish embryos. Moreover, both overexpression of efna5b mRNA and sema6ba mRNA could partially rescued motor neurons morphogenesis. These observations supported nexmifa as regulating axon morphogenesis of motor neurons in zebrafish. Taken together, nexmifa elicited crucial roles during motor neuron development by regulating the morphology of neuronal axons. Frontiers Media S.A. 2022-03-31 /pmc/articles/PMC9009263/ /pubmed/35431796 http://dx.doi.org/10.3389/fnmol.2022.848257 Text en Copyright © 2022 Zheng, Suo, Liu, Li, Wu and Ni. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Zheng, Yu-qin Suo, Gui-hai Liu, Dong Li, Hai-ying Wu, You-jia Ni, Hong Nexmifa Regulates Axon Morphogenesis in Motor Neurons in Zebrafish |
title | Nexmifa Regulates Axon Morphogenesis in Motor Neurons in Zebrafish |
title_full | Nexmifa Regulates Axon Morphogenesis in Motor Neurons in Zebrafish |
title_fullStr | Nexmifa Regulates Axon Morphogenesis in Motor Neurons in Zebrafish |
title_full_unstemmed | Nexmifa Regulates Axon Morphogenesis in Motor Neurons in Zebrafish |
title_short | Nexmifa Regulates Axon Morphogenesis in Motor Neurons in Zebrafish |
title_sort | nexmifa regulates axon morphogenesis in motor neurons in zebrafish |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9009263/ https://www.ncbi.nlm.nih.gov/pubmed/35431796 http://dx.doi.org/10.3389/fnmol.2022.848257 |
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