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Application of High-Throughput Imaging Mass Cytometry Hyperion in Cancer Research
Imaging mass cytometry (IMC) enables the in situ analysis of in-depth-phenotyped cells in their native microenvironment within the preserved architecture of a single tissue section. To date, it permits the simultaneous analysis of up to 50 different protein- markers targeted by metal-conjugated anti...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9009368/ https://www.ncbi.nlm.nih.gov/pubmed/35432353 http://dx.doi.org/10.3389/fimmu.2022.859414 |
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author | Le Rochais, Marion Hemon, Patrice Pers, Jacques-Olivier Uguen, Arnaud |
author_facet | Le Rochais, Marion Hemon, Patrice Pers, Jacques-Olivier Uguen, Arnaud |
author_sort | Le Rochais, Marion |
collection | PubMed |
description | Imaging mass cytometry (IMC) enables the in situ analysis of in-depth-phenotyped cells in their native microenvironment within the preserved architecture of a single tissue section. To date, it permits the simultaneous analysis of up to 50 different protein- markers targeted by metal-conjugated antibodies. The application of IMC in the field of cancer research may notably help 1) to define biomarkers of prognostic and theragnostic significance for current and future treatments against well-established and novel therapeutic targets and 2) to improve our understanding of cancer progression and its resistance mechanisms to immune system and how to overcome them. In the present article, we not only provide a literature review on the use of the IMC in cancer-dedicated studies but we also present the IMC method and discuss its advantages and limitations among methods dedicated to deciphering the complexity of cancer tissue. |
format | Online Article Text |
id | pubmed-9009368 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-90093682022-04-15 Application of High-Throughput Imaging Mass Cytometry Hyperion in Cancer Research Le Rochais, Marion Hemon, Patrice Pers, Jacques-Olivier Uguen, Arnaud Front Immunol Immunology Imaging mass cytometry (IMC) enables the in situ analysis of in-depth-phenotyped cells in their native microenvironment within the preserved architecture of a single tissue section. To date, it permits the simultaneous analysis of up to 50 different protein- markers targeted by metal-conjugated antibodies. The application of IMC in the field of cancer research may notably help 1) to define biomarkers of prognostic and theragnostic significance for current and future treatments against well-established and novel therapeutic targets and 2) to improve our understanding of cancer progression and its resistance mechanisms to immune system and how to overcome them. In the present article, we not only provide a literature review on the use of the IMC in cancer-dedicated studies but we also present the IMC method and discuss its advantages and limitations among methods dedicated to deciphering the complexity of cancer tissue. Frontiers Media S.A. 2022-03-31 /pmc/articles/PMC9009368/ /pubmed/35432353 http://dx.doi.org/10.3389/fimmu.2022.859414 Text en Copyright © 2022 Le Rochais, Hemon, Pers and Uguen https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Le Rochais, Marion Hemon, Patrice Pers, Jacques-Olivier Uguen, Arnaud Application of High-Throughput Imaging Mass Cytometry Hyperion in Cancer Research |
title | Application of High-Throughput Imaging Mass Cytometry Hyperion in Cancer Research |
title_full | Application of High-Throughput Imaging Mass Cytometry Hyperion in Cancer Research |
title_fullStr | Application of High-Throughput Imaging Mass Cytometry Hyperion in Cancer Research |
title_full_unstemmed | Application of High-Throughput Imaging Mass Cytometry Hyperion in Cancer Research |
title_short | Application of High-Throughput Imaging Mass Cytometry Hyperion in Cancer Research |
title_sort | application of high-throughput imaging mass cytometry hyperion in cancer research |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9009368/ https://www.ncbi.nlm.nih.gov/pubmed/35432353 http://dx.doi.org/10.3389/fimmu.2022.859414 |
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