Cargando…

Application of High-Throughput Imaging Mass Cytometry Hyperion in Cancer Research

Imaging mass cytometry (IMC) enables the in situ analysis of in-depth-phenotyped cells in their native microenvironment within the preserved architecture of a single tissue section. To date, it permits the simultaneous analysis of up to 50 different protein- markers targeted by metal-conjugated anti...

Descripción completa

Detalles Bibliográficos
Autores principales: Le Rochais, Marion, Hemon, Patrice, Pers, Jacques-Olivier, Uguen, Arnaud
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9009368/
https://www.ncbi.nlm.nih.gov/pubmed/35432353
http://dx.doi.org/10.3389/fimmu.2022.859414
_version_ 1784687253424963584
author Le Rochais, Marion
Hemon, Patrice
Pers, Jacques-Olivier
Uguen, Arnaud
author_facet Le Rochais, Marion
Hemon, Patrice
Pers, Jacques-Olivier
Uguen, Arnaud
author_sort Le Rochais, Marion
collection PubMed
description Imaging mass cytometry (IMC) enables the in situ analysis of in-depth-phenotyped cells in their native microenvironment within the preserved architecture of a single tissue section. To date, it permits the simultaneous analysis of up to 50 different protein- markers targeted by metal-conjugated antibodies. The application of IMC in the field of cancer research may notably help 1) to define biomarkers of prognostic and theragnostic significance for current and future treatments against well-established and novel therapeutic targets and 2) to improve our understanding of cancer progression and its resistance mechanisms to immune system and how to overcome them. In the present article, we not only provide a literature review on the use of the IMC in cancer-dedicated studies but we also present the IMC method and discuss its advantages and limitations among methods dedicated to deciphering the complexity of cancer tissue.
format Online
Article
Text
id pubmed-9009368
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-90093682022-04-15 Application of High-Throughput Imaging Mass Cytometry Hyperion in Cancer Research Le Rochais, Marion Hemon, Patrice Pers, Jacques-Olivier Uguen, Arnaud Front Immunol Immunology Imaging mass cytometry (IMC) enables the in situ analysis of in-depth-phenotyped cells in their native microenvironment within the preserved architecture of a single tissue section. To date, it permits the simultaneous analysis of up to 50 different protein- markers targeted by metal-conjugated antibodies. The application of IMC in the field of cancer research may notably help 1) to define biomarkers of prognostic and theragnostic significance for current and future treatments against well-established and novel therapeutic targets and 2) to improve our understanding of cancer progression and its resistance mechanisms to immune system and how to overcome them. In the present article, we not only provide a literature review on the use of the IMC in cancer-dedicated studies but we also present the IMC method and discuss its advantages and limitations among methods dedicated to deciphering the complexity of cancer tissue. Frontiers Media S.A. 2022-03-31 /pmc/articles/PMC9009368/ /pubmed/35432353 http://dx.doi.org/10.3389/fimmu.2022.859414 Text en Copyright © 2022 Le Rochais, Hemon, Pers and Uguen https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Le Rochais, Marion
Hemon, Patrice
Pers, Jacques-Olivier
Uguen, Arnaud
Application of High-Throughput Imaging Mass Cytometry Hyperion in Cancer Research
title Application of High-Throughput Imaging Mass Cytometry Hyperion in Cancer Research
title_full Application of High-Throughput Imaging Mass Cytometry Hyperion in Cancer Research
title_fullStr Application of High-Throughput Imaging Mass Cytometry Hyperion in Cancer Research
title_full_unstemmed Application of High-Throughput Imaging Mass Cytometry Hyperion in Cancer Research
title_short Application of High-Throughput Imaging Mass Cytometry Hyperion in Cancer Research
title_sort application of high-throughput imaging mass cytometry hyperion in cancer research
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9009368/
https://www.ncbi.nlm.nih.gov/pubmed/35432353
http://dx.doi.org/10.3389/fimmu.2022.859414
work_keys_str_mv AT lerochaismarion applicationofhighthroughputimagingmasscytometryhyperionincancerresearch
AT hemonpatrice applicationofhighthroughputimagingmasscytometryhyperionincancerresearch
AT persjacquesolivier applicationofhighthroughputimagingmasscytometryhyperionincancerresearch
AT uguenarnaud applicationofhighthroughputimagingmasscytometryhyperionincancerresearch