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Role of APOA1 in the resistance to platinum-based chemotherapy in squamous cervical cancer
BACKGROUND: To investigate the mechanism by which apolipoprotein A1 (APOA1) enhances the resistance of cervical squamous carcinoma to platinum-based chemotherapy. METHODS: Two cervical squamous carcinoma cell lines (SiHa and Caski) overexpressing APOA1 were constructed, treated with carboplatin, and...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9009492/ https://www.ncbi.nlm.nih.gov/pubmed/35421932 http://dx.doi.org/10.1186/s12885-022-09528-x |
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author | He, Yue Han, Su-Bin Liu, Yang Zhang, Jing-Jing Wu, Yu-Mei |
author_facet | He, Yue Han, Su-Bin Liu, Yang Zhang, Jing-Jing Wu, Yu-Mei |
author_sort | He, Yue |
collection | PubMed |
description | BACKGROUND: To investigate the mechanism by which apolipoprotein A1 (APOA1) enhances the resistance of cervical squamous carcinoma to platinum-based chemotherapy. METHODS: Two cervical squamous carcinoma cell lines (SiHa and Caski) overexpressing APOA1 were constructed, treated with carboplatin, and compared to normal control cells. RESULTS: In both SiHa and Caski cell lines, the clone-forming ability of CBP-treated cells was lower than that of untreated cells, and the change in the number of clones of overexpressing cells was lower than that of normal control cells (p < 0.05), indicating that APOA1 overexpression enhanced chemoresistance. A screen for APOA1 downstream proteins affecting platinum-based chemoresistance using Tandem Mass Tag revealed 64 differentially expressed proteins in SiHa cells, which were subjected to Gene Ontology (annotation, Kyoto Encyclopedia of Genes and Genomes enrichment, subcellular localization, structural domain annotation and enrichment, clustering, and interaction network analyses. Sixty-four differentially expressed proteins matching cancer-relavent association terms were screened and parallel response monitoring identified 29 proteins as possibly involved in the mechanism of platinum-based chemoresistance. CONCLUSIONS: Our analysis suggested that the mechanism may involve numerous regulatory pathways, including promoting tumor growth via the p38 MAPK signaling pathway through STAT1, promoting tumor progression via the PI3K signaling pathway through CD81 and C3, and promoting resistance to platinum-based chemotherapy resistance through TOP2A. The present study aimed to preliminarily explore the function and mechanism of APOA1 in platinum-based chemoresistance in cervical cancer, and the detailed mechanism needs to be further studied. |
format | Online Article Text |
id | pubmed-9009492 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-90094922022-04-15 Role of APOA1 in the resistance to platinum-based chemotherapy in squamous cervical cancer He, Yue Han, Su-Bin Liu, Yang Zhang, Jing-Jing Wu, Yu-Mei BMC Cancer Research BACKGROUND: To investigate the mechanism by which apolipoprotein A1 (APOA1) enhances the resistance of cervical squamous carcinoma to platinum-based chemotherapy. METHODS: Two cervical squamous carcinoma cell lines (SiHa and Caski) overexpressing APOA1 were constructed, treated with carboplatin, and compared to normal control cells. RESULTS: In both SiHa and Caski cell lines, the clone-forming ability of CBP-treated cells was lower than that of untreated cells, and the change in the number of clones of overexpressing cells was lower than that of normal control cells (p < 0.05), indicating that APOA1 overexpression enhanced chemoresistance. A screen for APOA1 downstream proteins affecting platinum-based chemoresistance using Tandem Mass Tag revealed 64 differentially expressed proteins in SiHa cells, which were subjected to Gene Ontology (annotation, Kyoto Encyclopedia of Genes and Genomes enrichment, subcellular localization, structural domain annotation and enrichment, clustering, and interaction network analyses. Sixty-four differentially expressed proteins matching cancer-relavent association terms were screened and parallel response monitoring identified 29 proteins as possibly involved in the mechanism of platinum-based chemoresistance. CONCLUSIONS: Our analysis suggested that the mechanism may involve numerous regulatory pathways, including promoting tumor growth via the p38 MAPK signaling pathway through STAT1, promoting tumor progression via the PI3K signaling pathway through CD81 and C3, and promoting resistance to platinum-based chemotherapy resistance through TOP2A. The present study aimed to preliminarily explore the function and mechanism of APOA1 in platinum-based chemoresistance in cervical cancer, and the detailed mechanism needs to be further studied. BioMed Central 2022-04-14 /pmc/articles/PMC9009492/ /pubmed/35421932 http://dx.doi.org/10.1186/s12885-022-09528-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research He, Yue Han, Su-Bin Liu, Yang Zhang, Jing-Jing Wu, Yu-Mei Role of APOA1 in the resistance to platinum-based chemotherapy in squamous cervical cancer |
title | Role of APOA1 in the resistance to platinum-based chemotherapy in squamous cervical cancer |
title_full | Role of APOA1 in the resistance to platinum-based chemotherapy in squamous cervical cancer |
title_fullStr | Role of APOA1 in the resistance to platinum-based chemotherapy in squamous cervical cancer |
title_full_unstemmed | Role of APOA1 in the resistance to platinum-based chemotherapy in squamous cervical cancer |
title_short | Role of APOA1 in the resistance to platinum-based chemotherapy in squamous cervical cancer |
title_sort | role of apoa1 in the resistance to platinum-based chemotherapy in squamous cervical cancer |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9009492/ https://www.ncbi.nlm.nih.gov/pubmed/35421932 http://dx.doi.org/10.1186/s12885-022-09528-x |
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