New alleles of the SWI/SNF chromatin remodeling complex gene phf-10

SWI/SNF chromatin remodeling complexes regulate many aspects of metazoan development and mutations in SWI/SNF genes are associated with diverse human diseases including cancer and alcohol use disorder. In C. elegans, SWI/SNF subunits are required for viability, somatic gonad development, and normal behavioral responses to ethanol. SWI/SNF complexes can be classified as BAF (BRG1/Brm-associated factors) or PBAF (Polybromo-associated BAF) based on their subunit composition. While there are loss-of-function alleles for most SWI/SNF family members, strong loss of function mutations have not previously been reported for the PBAF gene phf-10. Here we describe two new alleles of phf-10 that we generated using CRISPR/Cas9 genome editing.