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Characterization of N- and C-terminal endogenously tagged Tyrosyl-DNA phosphodiesterase 2 (TDPT-1) C. elegans strains

We have generated Tyrosyl-DNA phosphodiesterase 2 (TDPT-1) C. elegans strains where CRISPR/Cas9 was used to endogenously tag the protein at either the C- or N-terminus and validated the functionality of the resulting tagged TDPT-1 proteins. We have found that both the N-terminally tagged ( wrmScarle...

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Detalles Bibliográficos
Autores principales:	Bhandari, Nirajan, Pfeiffer, Shannon C., Jaramillo-Lambert, Aimee
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Caltech Library 2022
Materias:	New Finding
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9010224/ https://www.ncbi.nlm.nih.gov/pubmed/35622466 http://dx.doi.org/10.17912/micropub.biology.000540

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